cgi-bin/community/feature/200503.pl

   1 use strict;
   2 use CXGN::Page;
   3 my $page=CXGN::Page->new('200503.html','html2pl converter');
   4 my $stylesheet=<<END_STYLESHEET;
   5 <style type="text/css">
   6 <!--
   7     body {
   8         color: #000000;
   9         background-color: #ffffff;
  10     }
  11
  12     p {
  13         margin-left: 40px;
  14         text-align: justify;
  15     }
  16
  17     .footnote {
  18         font-size: small;
  19         /*width: 700px;*/
  20         text-align:center;
  21     }
  22
  23     .bibliography {
  24         text-indent: -20px;
  25     }
  26 -->
  27 </style>
  28 END_STYLESHEET
  29
  30 $page->header('Stack/Anderson Lab', undef, $stylesheet);
  31 print<<END_HEREDOC;
  32
  33   <center>
  34     <h1>Stack/Anderson Lab</h1>
  35   </center>
  36
  37   <p class="footnote"><img src="/static_content/community/feature/200503-1.png" border="0" width=
  38   "442" height="212" alt="Stack/Anderson Lab Lab" /><br />
  39   <strong>Front Row (left to right):</strong> Dr. Lorrie Anderson
  40   (assistant professor), Ann Lai (research associate), Jia Cheng
  41   (graduate student), Stephanie Lum (undergraduate). <strong>Back
  42   Row (left to right):</strong> Joe Qiao (graduate student), Kevin
  43   Su (undergraduate), Dr. Stephen Stack (professor), Suzanne Royer
  44   (research associate), Erin Benson (undergraduate), Song-Bin Chang
  45   (post doc). Absent from photo: Brittany Howard
  46   (undergraduate).</p>
  47
  48   <p>We work primarily with two proteinaceous structures: the
  49   synaptonemal complex (SC) and recombination nodules (RNs). The SC
  50   looks like a railroad track that is formed between synapsed
  51   homologous chromosomes, and RNs are 100 nm particles that occur
  52   on SCs at sites where crossing over occurs and where chiasmata
  53   will form later. We pioneered a technique for spreading complete
  54   sets of plant SCs for analysis by electron microscopy. We use
  55   this technique to determine the pattern and frequency of crossing
  56   over in wild type plants and in plants with chromosome
  57   aberrations such as translocations and inversions.</p>
  58
  59   <p class="footnote" style=
  60   "float:right; width:375; text-align:center;"><img src=
  61   "/static_content/community/feature/200503-2.png" border="0" width="350" height="190" alt=
  62   "FISH on tomato synaptonemal complexes" /><br />
  63   Fluorescence in situ hybridization (FISH) on spreads of tomato
  64   synaptonemal complexes.</p>
  65
  66   <p>We are particularly interested in the relation between genes
  67   and chromosome structure and the physical relation of
  68   recombination proteins to SCs and RNs. Ultimately we would like
  69   to determine the roles of SCs and RNs in crossing over and
  70   interference.</p>
  71
  72   <p>This is an exciting time in cytogenetics with so much new
  73   information, so many interesting questions, and so many new
  74   techniques and instruments to help find the answers. All that
  75   must be added for continued progress is imagination and hard
  76   work.</p>
  77
  78   <p>Currently we are involved in the following projects:</p>
  79
  80   <ol type="I" style="margin-left: 75px; text-align: justify;">
  81     <li>Fluorescence in situ hybridization (FISH) on spreads of
  82     tomato synaptonemal complexes (SCs = pachytene chromosomes) to
  83     determine the physical location of tomato DNA inserts in
  84     bacterial artificial chromosomes (BACs) (See chromosome image
  85     above.). FISH is integral to the tomato genome sequencing
  86     project 1) to keep the sequencing effort confined primarily to
  87     euchromatin, 2) to locate problem BACs, and 3) to define the
  88     size of gaps in chromosome walks.</li>
  89
  90     <li>Light and electron microscopic immunolocalization of
  91     proteins thought to be involved in recombination. This work
  92     concentrates on the timing of the appearance and disappearance
  93     of these proteins in relation to the SC and recombination
  94     nodules (RNs). See figure below.</li>
  95
  96     <li>Light and electron microscopic characterization of
  97     chiasmata and RNs in maize mutants that show changes in the
  98     rate and/or location of crossing over.</li>
  99
 100     <li>Spreading SCs from both plants and mammals to characterize
 101     the effects of chromosome aberrations such as translocations
 102     and inversions on the number and distribution of crossover
 103     events as indicated by RNs.</li>
 104   </ol>
 105
 106   <h2>Contact Information</h2>
 107
 108   <p class="footnote" style=
 109   "float:right; width:400; text-align:center; font-size: smaller;">
 110   <img src="/static_content/community/feature/200503-3.png" border="0" width="374" height="263" alt=
 111   "Tomato immunolabeling" /><br />
 112   Tomato immunolabeling: The photo is of a tomato spread (early
 113   zygotene, as the chromosomes are beginning to synapse)
 114   immunolabeled with Mre11 (green spots) and Smc1 (red). Mre11 is a
 115   DNA double-strand break repair protein and Smc1 is labeling the
 116   chromosome core (therefore enabling us to see the synaptonemal
 117   complex under fluorescent microscopy).</p>
 118
 119   <p><strong>Stephen Stack</strong><br />
 120   Department of Biology<br />
 121   Colorado State University<br />
 122   Fort Collins, Colorado 80523-1878<br />
 123   USA<br />
 124   Telephone: 970-491-6802<br />
 125   FAX: 970-491-0649<br />
 126   E-mail <a href=
 127   "mailto:sstack\@lamar.colostate.edu">sstack\@lamar.colostate.edu</a></p>
 128
 129   <p><strong>Lorinda Anderson</strong><br />
 130   Department of Biology<br />
 131   Colorado State University<br />
 132   Fort Collins, Colorado 80523<br />
 133   USA<br />
 134   Telephone: 970-491-4856<br />
 135   FAX: 970-491-0649<br />
 136   E-mail: <a href=
 137   "mailto:lorrie\@lamar.colostate.edu">lorrie\@lamar.colostate.edu</a></p><br clear="all" />
 138
 139
 140   <h2>Selected Publications</h2>
 141
 142   <p class="bibliography">Sherman, J.D. and S. M. Stack. 1995.
 143   Two-dimensional spreads of synaptonemal complexes from
 144   solanaceous plants. VI. High resolution recombination nodule map
 145   for tomato (Lycopersicon esculentum). Genetics 141:683-708</p>
 146
 147   <p class="bibliography">Peterson, D.G., H.J. Price, J.S.
 148   Johnston, and S.M. Stack. 1996. DNA content of heterochromatin
 149   and euchromatin in tomato (Lycopersicon esculentum) pachytene
 150   chromosomes. Genome 39:77-82</p>
 151
 152   <p class="bibliography">Peterson, D.G., K.S. Boehm, and S.M.
 153   Stack. 1997. Isolation of milligram quantities of nuclear DNA
 154   from tomato (Lycopersicon esculentum), a plant containing high
 155   levels of polyphenolic compounds. Plant Molec. Biol. Reporter
 156   15:148-153</p>
 157
 158   <p class="bibliography">Peterson, D.G., W.R. Pearson, S.M. Stack.
 159   1998. Characterization of the tomato (Lycopsersicon esculentum)
 160   genome using in vitro and in situ DNA reassociation. Genome
 161   41:346-356</p>
 162
 163   <p class="bibliography">Peterson, D.G., N.L.V. Lapitan, and S.M.
 164   Stack. 1999. Localization of single- and low-copy sequences on
 165   tomato synaptonemal complex spreads using fluorescence
 166   hybridization (FISH). Genetics 152:427-439</p>
 167
 168   <p class="bibliography">Stack, S.M. and L.K. Anderson. 2001. A
 169   model for chromosome structure during the mitotic and meiotic
 170   cell cycles. Chromosome Research 9:175-198</p>
 171
 172   <p class="bibliography">Anderson, L.K. and S.M. Stack 2001.
 173   Distribution of early recombination nodules on zygotene bivalents
 174   from plants. Genetics 159:1259-1269</p>
 175
 176   <p class="bibliography">Anderson, L.K., K.D. Hooker, and S.M.
 177   Stack 2001. The distribution of early recombination nodules on
 178   zygotene bivalents from plants. Genetics 159:1259-1269</p>
 179
 180   <p class="bibliography">Stack, S.M. and L.K. Anderson 2002.
 181   Crossing over as assessed by late recombination nodules is
 182   related to the pattern of synapsis and the distribution of early
 183   recombination nodules in maize. Chromosome Research
 184   10:329-345</p>
 185
 186   <p class="bibliography">Anderson, L.K. and S.M. Stack 2002.
 187   Meiotic recombination in plants. Current Genomics 3:507-526</p>
 188
 189   <p class="bibliography">Tenaillon, M.I., M.C. Sawkins, L.K.
 190   Anderson, S.M. Stack, J. Doebley, and B.S. Gaut 2002. Patterns of
 191   diversity and recombination along chromosome 1 of maize (Zea mays
 192   ssp. Mays L.) Genetics 162:1401-1413</p>
 193
 194   <p class="bibliography">Anderson, L.K., G.C. Doyle, B. Brigham,
 195   J. Carter, K.D. Hooker, A. Lai, M. Rice, and S.M. Stack. 2003.
 196   High resolution Crossover maps for each bivalent of Zea mays
 197   using recombination nodules. Genetics 165:849-865.</p>
 198
 199   <p class="bibliography">Anderson, L.K., N. Salameh, H.W. Bass,
 200   L.C. Harper, W.Z. Cande, G. Weber, and S.M. Stack. 2004.
 201   Integrating genetic linkage maps with pachytene chromosome
 202   structure in maize. Genetics 166:1923-1933.</p>
 203 END_HEREDOC
 204 $page->footer();