2 <& /page/page_title.mas, title=>"SSR Marker Help" &>
4 <p>While microsatellites or simple sequence repeats (SSRs)
5 normally occur in non-coding regions of the genome, they can
6 also occur in either 3' or 5' UTRs of ESTs or even in coding
7 regions (usually triplet repeats). For this reason, the tomato
8 and potato EST sequences contained in SGN were screened for
9 microsatellite sequence motifs in May 2001 using analytical
10 tools developed by Genomics Edge Technologies, Inc; St. Louis,
11 Missouri. The purpose was to identify SSRs that might be useful
12 a polymorphic molecular markers genetic and breeding studies in
13 tomato. The search parameters were for all possible repeat
14 motifs 2 bp to 6 bp with repeat blocks > 20 bp. The search
15 algorithms allowed for imperfect repeats as well as complex
18 <p>For those wishing to test SSRs for mapping/polymorphism
19 studies, it is recommended (based on empirical testing) to start
23 <li>dinucleotides > 12 repeats</li>
25 <li>trinucleotide > 8 repeats</li>
27 <li>tetra to hexonuclotides > 6 repeats</li>
30 <a href="/search/markers/markersearch.pl?searchtype=startswith&name=&submit=search&mapped=on&confidence=0&offsetstart=&offsetend=&chromosomes=all&species=all&maps=all&types=SSR&techniques=all">See all SSR markers in marker search results</a><br />
31 <a href="/documents/markers/microsat_list.txt">Old text version SSR list</a>