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105 <div style="width: 80%; margin: 0 auto">
106 <& /page/page_title.mas, title => 'A project to catalog and study the dynamic properties of the cell wall proteome (the secretome), using tomato as a model system.' &>
109 <div id="secretom_summary" style="float: left; width: 50%">
110 <&| /page/info_section.mas, title => 'Summary' &>
113 The <a class="info_link" href="/secretom/detail/cell_wall">plant cell wall/apoplast</a> plays a fundamental role
114 in many aspects of growth, development, and responses to environmental
115 conditions and assault by pathogens and insects. It is therefore not
116 surprising that a substantial portion of the plant proteome is
117 localized in the cell wall/apoplast. However, while major initiatives
118 are now underway to map the proteomes of many plant organelles, such
119 as the <a href="http://ppdb.tc.cornell.edu/dbsearch/subproteome.aspx">
120 chloroplast</a>, the cell wall proteome, or "secretome", is far
121 less well characterized than those of other subcellular compartments.
124 <img width="250" style="display: block; margin: 0 auto" src="/img/secretom/proteome.png" />
127 <strong>What is the "secretome"?</strong> The term "secretome" is
128 generally used to refer to the population of proteins that are
129 secreted outside the plasma membrane of a cell, but can also be used
130 to describe the subset of the proteome that enter the <a href="/secretom/detail/secretion_path">secretory
135 <strong>The purpose of this NSF-funded project</strong> is to
136 catalog and study the dynamic properties of
137 the cell wall proteome, or secretome, using tomato (<i>Solanum
138 lycopersicum</i>) as a model system.
143 <strong>Why study the "secretome"?</strong> Proteomic analysis of cellular
144 compartments reveals the subcellular locations of a specific protein,
145 or protein complex, which in turn can be a valuable step towards
146 understanding protein function. Localization information also helps in
147 the identification of protein interactions and targeting pathways, and
148 peptide motifs and structural signatures that can direct proteins to
149 various cellular locations. In addition the analysis of a subcellular
150 proteome has the advantage of requiring "biological
151 pre-fractionation," which can substantially enhance the detection of
152 lower-abundance proteins.
157 <div style="float: right; width: 50%">
159 <div class="projects">
160 <&| /page/info_section.mas, title => 'Projects', subtitle => 'download datasets: <a href="ftp://ftp.solgenomics.net/secretom/">[SGN FTP site]</a>' &>
162 <a class="project" href="/secretom/detail/prediction">Computational Prediction of the Plant Secretome</a>
163 <a class="project" href="/secretom/detail/functional_screens">Functional Screens for Secreted Proteins</a>
164 <a class="project" href="/secretom/detail/glycoproteome">The Tomato Fruit Glycoproteome</a>
165 <a class="project" href="/secretom/detail/proteomics">Tomato Comparative and Quantitative Proteomics</a>
166 <a class="project" href="/secretom/detail/profiling">Integrated Proteome/Transcriptome Profiling of Tomato Fruit</a>
167 <a class="project" href="/secretom/detail/cuticle">The Tomato Fruit Cuticular Cell Wall Proteome</a>
168 <a class="project" href="/secretom/detail/phytophthora_interaction">The Secretome of Phytophthora/Tomato Interactions</a>
169 <a class="project" href="/secretom/detail/spinoffs">Spin-off Projects and Collaborations</a>
173 <div class="resources">
174 <&| /page/info_section.mas, title => 'Resources' &>
176 <a href="ftp://ftp.solgenomics.net/secretom/">
177 <span class="secretom">secretom</span> datasets (<span style="text-decoration: underline">SGN FTP</span>)
179 <a href="/secretom/publications">
180 <span class="secretom">secretom</span> publications
183 <div class="secretary">
184 <a href="/secretom/secretary">Use SecreTary</a>
185 A tool for predicting secreted proteins.
191 <&| /page/info_section.mas, title => 'News' &>
193 <div style="float: left; width: 50%">
194 <&| /page/info_section.mas, title => 'Job Openings', is_subsection => 1 &>
196 The Rose lab is currently seeking a postdoctoral research associate with
197 an interest in plant proteomics, or fruit development and ripening.
198 Experience in mass spectrometry and plant molecular biology would
199 be an advantage, and good communication skills are critical.
200 Please contact Joss Rose if interested:
201 <a href="mailto:jr286@cornell.edu">jr286@cornell.edu</a>
207 <div style="width: 50%; float: right" id="education">
209 <&| /page/info_section.mas, title => 'Education, Training, and Outreach', is_subsection => 1 &>
210 <img style="border: 1px solid black; display: block; margin: 15px auto 0 auto" src="/img/secretom/tomato_bath.png" />
212 <a href="/secretom/training">Education and training
213 opportunities</a> in plant proteomics and cell wall
214 biology are available as part of <span class="secretom">Secretom</span>.
217 <p><a href="/secretom/outreach">Internships</a> are also available.</p>
223 <&| /page/info_section.mas, title => 'People' &>
225 <li>Joss Rose <a href="http://labs.plantbio.cornell.edu/rose/">[Lab]</a></li>
226 <li>Ted Thannhauser <a href="http://www.ars.usda.gov/PandP/docs.htm?docid=9634">[USDA Personal]</a></li>
227 <li>Jim Giovannoni <a href="http://www.bti.cornell.edu/JimGiovannoni.php?page=Research#page=ResearchSummary">[BTI Personal]</a></li>
228 <li>Lukas Mueller <a href="http://solgenomics.net/about">[SGN]</a></li>
229 <li>Zhangjun Fei <a href="http://www.bti.cornell.edu/ZhangjunFei.php?page=Research#page=ResearchSummary">[BTI Personal]</a></li>
233 <&| /page/info_section.mas, title => 'Funding' &>
236 <td><a style="background: none" href="http://www.nsf.gov"><img border="0" src="/documents/img/secretom/NSF_Logo.jpg" width="100" height="100"/></a></td>
237 <td style="vertical-align: middle; padding-left: 1em">
238 <p>This project and database are funded by National Science Foundation Grant
239 <a href="http://nsf.gov/awardsearch/showAward.do?AwardNumber=0606595">0606595</a>.
243 For questions and comments regarding the SecreTom project, please
244 contact Jocelyn Rose at <a href="mailto:jr286@cornell.edu">jr286@cornell.edu</a>.
251 <&| /page/info_section.mas, title => 'Related Sites' &>
254 <li><a href="http://www.ccrc.uga.edu/~mao/cellwall/main.htm">Plant Cell Wall Overview</a></li>
255 <li><a href="http://cell.ccrc.uga.edu/~mao/wallmab/Home/Home.php">Cell Wall Antibodies</a></li>
256 <li><a href="http://cellwall.genomics.purdue.edu/">Cell Wall Genomics</a></li>
257 <li><a href="http://csbl1.bmb.uga.edu/pDAWG">Plant cell wall genes (pDAWG)</a></li>
258 <li><a href="http://bioweb.ucr.edu/Cellwall">Cell Wall Navigator</a></li>
259 <li><a href="http://www.cazy.org">Carbohydrate Active Enzymes</a></li>