1 BLASTP 2.1.3 [Apr-11-2001]
4 Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
5 Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
6 "Gapped BLAST and PSI-BLAST: a new generation of protein database search
7 programs", Nucleic Acids Res. 25:3389-3402.
9 Query= gi|1786183|gb|AAC73113.1| (AE000111) aspartokinase I,
10 homoserine dehydrogenase I [Escherichia coli]
14 4289 sequences; 1,358,990 total letters
16 Searching..................................................done
19 Sequences producing significant alignments: (bits) Value
21 gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogen... 1567 0.0
22 gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydr... 332 1e-91
23 gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [E... 184 3e-47
24 gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli] 42 3e-04
26 >gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogenase I [Escherichia
30 Score = 1567 bits (4058), Expect = 0.0
31 Identities = 806/820 (98%), Positives = 806/820 (98%)
33 Query: 1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
34 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA
35 Sbjct: 1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
37 Query: 61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
38 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA
39 Sbjct: 61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
41 Query: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
42 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP
43 Sbjct: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
45 Query: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
46 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV
47 Sbjct: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
49 Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
50 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD
51 Sbjct: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
53 Query: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
54 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF
55 Sbjct: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
57 Query: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
58 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL
59 Sbjct: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
61 Query: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQXXXXXXXXXXXXXXAL 480
62 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQ AL
63 Sbjct: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL 480
65 Query: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
66 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL
67 Sbjct: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
69 Query: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
70 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR
71 Sbjct: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
73 Query: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
74 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA
75 Sbjct: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
77 Query: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
78 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA
79 Sbjct: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
81 Query: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
82 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF
83 Sbjct: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
85 Query: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
86 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV
87 Sbjct: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
90 >gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydrogenase II
94 Score = 332 bits (850), Expect = 1e-91
95 Identities = 243/821 (29%), Positives = 403/821 (48%), Gaps = 44/821 (5%)
97 Query: 5 KFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNI 64
98 KFGG+S+A+ + +LRVA I+ ++ + V+SA TN L+ ++ + + + + +
99 Sbjct: 16 KFGGSSLADVKCYLRVAGIMAEYSQPDDMM-VVSAAGSTTNQLINWLKLSQTDRLSAHQV 74
101 Query: 65 SDAERIF-AELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINAALI 123
102 R + +L++GL A+ L + FV + GI+ D++ A ++
103 Sbjct: 75 QQTLRRYQCDLISGLLPAEEADSL--ISAFVSDLERLAALLDSGIN------DAVYAEVV 126
105 Query: 124 CRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIPADH 183
106 GE S +M+ VL +G +D E L A + + E ++ H
107 Sbjct: 127 GHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAER---AAQPQVDEGLSYPLLQQLLVQH 183
109 Query: 184 ---MVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
110 +++ GF + N GE V+LGRNGSDYSA + A IW+DV GVY+ DPR+V
111 Sbjct: 184 PGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPRKV 243
113 Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
114 DA LL + EA EL+ A VLH RT+ P++ +I ++ + P G++R
115 Sbjct: 244 KDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQ-----GSTRI 298
117 Query: 301 EDELP----VKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEY 356
118 E L + +++ +++ + P + + + RA++ + + +
119 Sbjct: 299 ERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQ 358
121 Query: 357 SISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKF 416
122 + FC A + + E GL L + + LA++++VG G+ +F
123 Sbjct: 359 LLQFCYTSEVADSALKILDEA-------GLPGELRLRQGLALVAMVGAGVTRNPLHCHRF 411
125 Query: 417 FAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQXXXXXXXXXXXX 476
126 + L + Q S+ V+ + ++ HQ +F ++
127 Sbjct: 412 WQQLKGQPVEFTW--QSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNI 469
129 Query: 477 XXALLEQLKRQQSWLKNKH-IDLRVCGVANSKALLTNVHGLN----LENWQEELAQAKEP 531
130 LE R+QS L + + + GV +S+ L + GL+ L + +E + E
131 Sbjct: 470 GSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEE 529
133 Query: 532 FNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQ 591
134 L ++ + + V++D T+SQ +ADQY DF GFHV++ NK A S + Y Q
135 Sbjct: 530 ----SLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQ 585
137 Query: 592 LRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGM 651
138 + A EK+ R +LY+ VGAGLP+ +++L+++GD ++ SGI SG+LS++F + D +
139 Sbjct: 586 IHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSV 645
141 Query: 652 SFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNA 711
142 F+E A + G TEPDPRDDLSG DV RKL+ILARE G +E + +E ++PA
143 Sbjct: 646 PFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEG 705
145 Query: 712 EGDVAAFMANLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKV 771
146 G + F N +L++ R+ AR+ G VLRYV D +G RV + V + PL +
147 Sbjct: 706 -GSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASL 764
149 Query: 772 KNGENALAFYSHYYQPLPLVLRGYGAGNDVTAAGVFADLLR 812
150 +N A S +Y+ PLV+RG GAG DVTA + +D+ R
151 Sbjct: 765 LPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQSDINR 805
154 >gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [Escherichia coli]
157 Score = 184 bits (467), Expect = 3e-47
158 Identities = 142/471 (30%), Positives = 228/471 (48%), Gaps = 41/471 (8%)
160 Query: 3 VLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQ---- 58
161 V KFGGTSVA+ + R ADI+ S+A V VLSA A ITN LVA+ E G+
162 Sbjct: 6 VSKFGGTSVADFDAMNRSADIVLSDANVRLV--VLSASAGITNLLVALAEGLEPGERFEK 63
164 Query: 59 -DALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCP-- 115
165 DA+ NI F + + + + +I+ +L I++L +
166 Sbjct: 64 LDAIRNIQ-------------------FAILERLRYPNVIREEIERLLENITVLAEAAAL 104
168 Query: 116 ---DSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTR 172
169 ++ L+ GE MS + +L R D + + + + DIA
170 Sbjct: 105 ATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIAALAE 164
172 Query: 173 RIAASRIPA--DHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVD 230
173 A +P + +V+ GF KG LGR GSDY+AA+LA L A +IWTDV
174 Sbjct: 165 LAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVDIWTDVP 224
176 Query: 231 GVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
177 G+YT DPR V A+ + +++ EA E++ FGAKVLHP T+ P + IP + ++ +P+A
178 Sbjct: 225 GIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPRA 284
180 Query: 291 PGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLIT 350
181 GTL+ ++ E+ + ++ N + ++ M G A VF ++R ISV LIT
182 Sbjct: 285 GGTLV-CNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLIT 343
184 Query: 351 QSSSEYSISFCVPQSDCV-RAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTL 409
185 +SE S++ + + + + + +EL L + V E LA+++++G+ +
186 Sbjct: 344 --TSEVSVALTLDTTGSTSTGDTLLTQSLLMEL--SALCRVEVEEGLALVALIGNDLSKA 399
188 Query: 410 RGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLF 460
189 G+ + F L NI + I G+S ++ +V +DA V+ H LF
190 Sbjct: 400 CGVGKEVFGVLEPFNIRM--ICYGASSHNLCFLVPGEDAEQVVQKLHSNLF 448
193 >gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli]
196 Score = 41.6 bits (96), Expect = 3e-04
197 Identities = 28/97 (28%), Positives = 44/97 (44%), Gaps = 8/97 (8%)
199 Query: 199 LVVLGRNGSDYSAAVLAACLR-----ADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQE 253
200 +++ G+ + AACLR AD T VDGV+T DP + P A + + ++Y E
201 Sbjct: 132 VILSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPAKDPTATMYEQLTYSE 191
203 Query: 254 AMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
205 Sbjct: 192 VLEKE---LKVMDLAAFTLARDHKLPIRVFNMNKPGA 225
209 Posted date: Dec 6, 2001 1:58 PM
210 Number of letters in database: 1,358,990
211 Number of sequences in database: 4289
222 Gap Penalties: Existence: 11, Extension: 1
223 Number of Hits to DB: 2022122
224 Number of Sequences: 4289
225 Number of extensions: 82424
226 Number of successful extensions: 256
227 Number of sequences better than 1.0e-03: 4
228 Number of HSP's better than 0.0 without gapping: 3
229 Number of HSP's successfully gapped in prelim test: 1
230 Number of HSP's that attempted gapping in prelim test: 243
231 Number of HSP's gapped (non-prelim): 4
233 length of database: 1,358,990
234 effective HSP length: 47
235 effective length of query: 773
236 effective length of database: 1,157,407
237 effective search space: 894675611
238 effective search space used: 894675611