1 ID AF036895 standard; DNA; INV; 3693 BP.
7 DT 22-DEC-1997 (Rel. 54, Created)
8 DT 03-MAR-2000 (Rel. 62, Last updated, Version 6)
10 DE Chironomus thummi special lobe-specific silk protein ssp160 gene, complete
16 OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera;
17 OC Endopterygota; Diptera; Nematocera; Chironomoidea; Chironomidae;
18 OC Chironominae; Chironomus.
24 RA Hoffman R.T., Schmidt E.R., Case S.T.;
25 RT "A cell-specific glycosylated silk protein from Chironomus thummi salivary
26 RT glands. Cloning, chromosomal localization, and characterization of cDNA";
27 RL J. Biol. Chem. 271(16):9809-9815(1996).
33 RA Berezikov E., Blinov A.G., Scherbik S., Cox C.K., Case S.T.;
34 RT "Structure and polymorphism of the Chironomus thummi gene encoding special
35 RT lobe-specific silk protein, ssp160";
36 RL Gene 223(1-2):347-354(1998).
40 RA Berezikov E., Blinov A.G., Scherbik S.S., Cox C.C., Case S.T.;
42 RL Submitted (05-DEC-1997) to the EMBL/GenBank/DDBJ databases.
43 RL Biochemistry, University of Mississippi Medical Center, 2500 North State
44 RL Street, Jackson, MS 39216-4505, USA
46 CC On Mar 4, 1998 this sequence version replaced gi:2707296.
48 FH Key Location/Qualifiers
52 FT /db_xref="taxon:7154"
53 FT /organism="Chironomus thummi"
56 FT /dev_stage="larval"
57 FT /cell_type="lobe-specific secretory"
58 FT /tissue_type="salivary gland"
61 FT TATA_signal 169..172
63 FT mRNA join(198..288,604..759,825..2408,2874..3129,3188..3241,
66 FT /product="special lobe-specific silk protein ssp160"
69 FT CDS join(235..288,604..759,825..2408,2874..3129,3188..3241,
72 FT /db_xref="UniProt/TrEMBL:O44416"
73 FT /note="160 kDa secretory protein"
75 FT /product="special lobe-specific silk protein ssp160"
76 FT /protein_id="AAD11516.1"
77 FT /translation="MNIKVILVCALVAIFFAQVAEGGPIANFVGFIISLLFSLFEVMLS
78 FT VVFDVKSFTSLSNATANATMPGFASSVGGGRFTVIMKGTFNLIAMISANIQAIQSGSGS
79 FT ASSNSSSSANSTTSSNSTTSSNSTTSSNSTTSSNSTSSGLTTGASVVSLIDTCAWVYQD
80 FT SSVGIAYLMVSILALFYGQSVSAPPYADLGIPALPANTSGAGVPQSVQIKAAITYINVT
81 FT INFITLTGQQFEDLQGPVTTDCGCPNTTSVAPLVAEWEAIMAALEAFASGSASSNSTSN
82 FT STSTSNSTTTSNSTTTTNSTTSTNSTSSSNSTTIAGSIDIAANLTVALQNLQALLMQEA
83 FT TCAPCLAANAKKSGVREFGPCKAAGSSCARSGQRKVKRKARLEKMRAKSRRAVGNRKGS
84 FT MKKRVRSRAKKFGKAAKSGVRRYRKNIKFVYIPPVMASLNAYAALMASLSDSISYQSES
85 FT ALNSTDSACNSTSNSTDEAVINATTAVTDMFVNFTAMVINNTVAHPNCTQYADMALSMV
86 FT SQINEQIIACGSQSDSAQSSIYANVTISIVAMAQEYNNFASMSDKCTRSFANSWLWMYI
87 FT KWVFYRMGMTSGVPNFLACQTKAQSSLTAFLASFNATVSATISAASANNSEVQSSEAAC
88 FT IESSLAEAAVILEMFEAAYQNCTDPGSVTVPAETTTTTSTTTTTTTTTTTAAPTTTTTK
89 FT AANAPFTYPLCTLIMSTTCSLGGAGCTYPLISSAGCCPSGKTLNTGLGGRGCCK"
92 FT polyA_signal order(3520..3523,3532..3537,3539..3544)
95 SQ Sequence 3693 BP; 1171 A; 768 C; 633 G; 1121 T; 0 other;
96 aagctttcaa ctctcattta aacctaaata agcacatcat atccacaatt catcagccaa 60
97 taaaaattca ctttatttta atccaatcaa agaaagtttc acgatgtcaa aaacattcgt 120
98 ggatgtcccg tcaagtggaa ggagaaacga aaattcacga aatttcacaa taaaaagtga 180
99 gtaagttgtg tggtccatgt catatcgatc taatttatcg aatagtgata aaatatgaat 240
100 attaaagtga tcttagtgtg cgccttggtt gcgatcttct ttgcacaggt aagttggtaa 300
101 cttgggttgg gatcttcttt aaatttgact agctttgtac aagatatgag ttgtatagtc 360
102 tgtagttgta gtgtcatggg ttgtatactg tcttgtaggc taggttttgc ggctcttgca 420
103 caaatatatc ttaaaaagat cttaaaagtc aacgagagtg ttcaataacg tgatcaataa 480
104 caagcccatg atacgtctca gctccttaaa tccaccaaat atgactcata tacagacaca 540
105 accctaatct taaagtacta agcaatttta atcacaattt taaattttcc acatcttatc 600
106 caggttgcag aaggcggacc aatcgccaat tttgtaggct tcatcatctc cctcttgttc 660
107 tccttgtttg aagtcatgct gtcagttgtg ttcgatgtca agtcgttcac aagcttgtca 720
108 aatgccacag caaatgcaac gatgcctggt tttgcatctg tgagtatttt tagctatagc 780
109 ttgacctgca aaatccttga taaatatgtt gattccattt ctagagcgtt ggtggtggtc 840
110 gcttcacagt catcatgaaa ggaacattca atttgattgc catgatatcg gcaaatattc 900
111 aagccattca gtcaggatca ggatcagcat cgtctaattc ctcatcaagt gctaattcta 960
112 caacatcctc aaactcaaca acaagctcaa actcaacgac atcctcgaac tcaacaacaa 1020
113 gctcaaactc tacatccagt ggattaacaa ccggtgctag cgttgtaagc ttgattgata 1080
114 cctgtgcttg ggtctatcag gacagttcag ttggaattgc ctacttgatg gtctcaattt 1140
115 tggcactttt ctatggacaa tctgtctcag caccgccata tgctgatctt ggtataccag 1200
116 ctctaccagc aaatacctct ggtgctggag ttccacaatc tgtacaaatt aaagcagcaa 1260
117 ttacttacat caatgttact attaacttta ttaccttaac tggtcaacaa tttgaagatt 1320
118 tacaaggtcc agttaccaca gattgtggat gtccaaatac aactagtgtt gcgccacttg 1380
119 ttgctgaatg ggaagccata atggctgctc ttgaagcttt cgctagtgga tcagcatcat 1440
120 ctaattctac atctaattca acatcaacaa gtaactcaac aacgacaagc aactcaacaa 1500
121 ctacgacaaa ttcaacaact tcaactaatt caacatcctc gtcaaattcg acaacaattg 1560
122 ctggatctat tgacattgct gctaatctta cagttgccct ccagaacctc caagccttgc 1620
123 tcatgcaaga agctacttgt gctccatgcc tagctgcaaa tgccaaaaag agtggtgttc 1680
124 gtgaatttgg accatgtaaa gctgctggct catcatgtgc tagatcagga caaagaaaag 1740
125 tcaagcgtaa ggcacgattg gagaagatgc gtgccaagtc ccgtcgtgca gtcggtaaca 1800
126 gaaaaggatc aatgaagaag cgcgtccgta gtcgtgcaaa gaaattcgga aaggctgcaa 1860
127 agtcaggagt tagacgatac cgcaagaaca tcaagtttgt ctacattcca ccagtcatgg 1920
128 cttcacttaa tgcatatgca gcattgatgg catctttaag tgacagcatc tcatatcaat 1980
129 ctgaatcggc tttgaactca acagactctg catgtaactc aacatcaaac agcacagatg 2040
130 aggctgtaat taatgcaaca accgctgtta ctgatatgtt tgtcaacttt actgctatgg 2100
131 tcatcaataa tactgttgca catccaaatt gtactcaata tgctgacatg gctctttcaa 2160
132 tggtctctca aatcaacgag caaatcattg catgcggaag tcagtctgat tctgctcagt 2220
133 catcaatcta tgcaaatgtt acaatcagta ttgttgcaat ggctcaagaa tacaataact 2280
134 ttgcatctat gtctgataag tgtaccagat catttgccaa tagttggttg tggatgtaca 2340
135 ttaagtgggt cttctatcga atgggaatga cttctggagt tcctaacttc ctcgcttgtc 2400
136 aaactaaggt ttgtgattta cttaacaaat acttgaaact aaacgtatcc cttagaactg 2460
137 aacttatcat tagaaattga acttatcctt taaaaactga acttatcccg agaaaagaaa 2520
138 tgtatcctta gaaacgaaat cggttttaaa aactgagctt attcttcagg aactaaactc 2580
139 atccttagaa actgaccata tccttaaaaa ctgaacgtat tcttagaagc taactcatta 2640
140 ttcagaaact gaacttgacc ttagaaactg aacatattct tcggactaaa ctcatcctaa 2700
141 gaaactgacc gtatccttaa aaactgaact tagaagctga actcattatt cagaaactga 2760
142 agttcaccct tagaaactga acttattctt caggaactaa acttatcctt tgaaactgaa 2820
143 cttatcctta aaaactgatt ttactgagtc cttctttaat ccttcattaa taggctcaaa 2880
144 gcagtcttac agcattcttg gcatcattta atgccacagt cagtgccacg atttcagcag 2940
145 caagtgcaaa taattctgaa gttcaaagct ctgaagcagc ttgtattgaa agtagtcttg 3000
146 cggaagcagc cgttatttta gaaatgtttg aggcagcata tcaaaactgt acagatccag 3060
147 gatccgtaac agttccagca gagactacaa caacgaccag tacaaccacc actacaacta 3120
148 ccacaacaag taggttaaaa ttggaaaagt ttagaatttt gtgtgcaatt tttttaattt 3180
149 tttacagcaa ccgcagcacc aacaacaacc acaactaagg ctgctaatgc accattcaca 3240
150 tgtaagatat gaaatcaaat tgtgactttt tctaagaaat tgtttccttt ttttctagat 3300
151 ccattgtgta ctttgatcat gtcaacaaca tgctcactgg gtggagcagg atgtacatat 3360
152 ccacttatct catctgctgg ctgctgtcca tctggcaaaa cattaaacac tggtctcggt 3420
153 ggacgtggtt gttgcaaata aaatctacag cgacagttaa attgaagcaa ttattttttc 3480
154 ctatttcttg atgtagaaca ttttttgtac ttaattttga taaaatgtgg cattaaacat 3540
155 ataatctgaa atgtggacga gaaactttat tttaaactat ttatttattt tttaatcttt 3600
156 tcagaaactc aatttatccc ataggaactg aacttatcct taataaactg gtcttatctt 3660
157 aagcaacaaa acgttttctt ttcaactgaa ttc 3693