bin/bp_search2alnblocks

   1 #!/usr/bin/perl
   2 # Author:      Jason Stajich <jason-at-bioperl-dot-org>
   3 # Description: Turn SearchIO parseable report(s) into a set of Aligned blocks
   4 #
   5
   6 =head1 NAME
   7
   8 bp_search2alnblocks - Turn SearchIO parseable reports(s) into a set of aligned blocks
   9
  10 =head1 SYNOPSIS
  11
  12   bp_search2alnblocks --minid PERCENTID --minlen LEN --minevalue EVALUE file1.
  13   blast file2.blast ...> out.fas
  14
  15 =head1 DESCRIPTION
  16
  17 This script will parse and filter BLAST (or other formats
  18 L<Bio::SearchIO> can parse) output and format the alignment as blocks
  19 of alignments based on the HSPs.  Note this can only work if the input
  20 file parsed contains the necessary.
  21
  22 Typically this can be used to turn BLAST output into a FASTA alignment format for input into the QRNA comparative gene finder for RNA genes (E.Rivas).
  23
  24 =head1 OPTIONS
  25
  26  --maxevalue   Maximum E-value for an HSP
  27  --minevalue   Minimum E-value for an HSP
  28  --minlen      Minimum length of an HSP [default 0]
  29  --maxid       Maximum Percent Id [default 100]
  30                (to help remove sequences which are really close)
  31  --minid       Minimum Percent Identity for an HSP [default 0]
  32  -i/--input    An optional input filename (expects input on STDIN by default)
  33  -o/--output   An optional output filename (exports to STDOUT by default)
  34  -f/--format   Specify a different Search Alignment format-
  35                {fasta, axt, waba, blast, blastxml} are all permitted
  36                although the format must have actual alignment
  37                sequence for this script to work
  38                See L<Bio::SearchIO> for more information.
  39  -of/--outformat Output format for the alignment blocks, anything
  40                L<Bio::AlignIO> supports.
  41  -v/--verbose  Turn on debugging
  42
  43 =head1 AUTHOR - Jason Stajich
  44
  45 Jason Stajich, jason-at-bioperl-dot-org.
  46
  47 =cut
  48
  49
  50 use strict;
  51 use warnings;
  52
  53 use Bio::SearchIO;
  54 use Bio::AlignIO;
  55 use Math::BigFloat;
  56 use Getopt::Long;
  57
  58 my $Usage = 'search2alnblocks --minid PERCENTID --minlen LEN --maxevalue EVALUE file1.blast file2.blast ... > blocks.fas';
  59
  60 my ($min_id,$min_len,$max_id,$max_len,$max_evalue,$min_evalue,$format,
  61     $outformat,$verbose,$input,$output);
  62 $min_id  = 0;
  63 $max_evalue = 0;
  64 $min_evalue = undef;
  65 $min_len = 0;
  66 $format = 'blast';
  67 $outformat= 'fasta';
  68 GetOptions(
  69            'minid:s'      => \$min_id,
  70            'maxid:s'      => \$max_id,
  71            'minlen:s'     => \$min_len,
  72            'maxlen:s'     => \$max_len,
  73            'minevalue:s'  => \$min_evalue,
  74            'maxevalue:s'  => \$max_evalue,
  75            'f|format:s'   => \$format,
  76            'i|input:s'    => \$input,
  77            'o|output:s'   => \$output,
  78            'of|oformat:s' => \$outformat,
  79            'v|verbose'    => \$verbose,
  80            'h|help'       => sub { system('perldoc', $0);
  81                                    exit(0) },
  82            );
  83 $max_evalue =~ s/^e/1e/;
  84
  85 # if $input is undef then will read from STDIN
  86 my $parser =  new Bio::SearchIO(-format => $format,
  87                                 -file   => $input,
  88                                 -verbose=> $verbose);
  89 my $out;
  90
  91 if( $output ) {
  92     $out = new Bio::AlignIO(-format => $outformat,
  93                             -file   => ">$output");
  94 } else {
  95     $out = new Bio::AlignIO(-format => $outformat);
  96 }
  97
  98 my $id = 1;
  99 while( my $r = $parser->next_result ) {
 100     while( my $hit = $r->next_hit ) {
 101         while( my $hsp = $hit->next_hsp ) {
 102             my $hsplen = $hsp->length('total');
 103             next if( $min_len && $hsplen < $min_len);
 104             my $pid = $hsp->percent_identity;
 105             next if( ($min_id && $pid < $min_id) ||
 106                      ($max_id && $pid > $max_id ) );
 107             next if( defined $min_evalue &&
 108                      $hsp->evalue > $min_evalue );
 109             next if( $max_evalue &&
 110                      $hsp->evalue < $max_evalue);
 111             $verbose && $hsp->verbose($verbose);
 112             my $aln = $hsp->get_aln();
 113             my @seqs;
 114             foreach my $seq ( $aln->each_seq ) {
 115                 if( $seq->display_id =~ /(\S+)[\/\_](\d+)\-(\d+)/ ) {
 116                     $seq->display_id($1);
 117                     $seq->start($seq->start + $2 - 1);
 118                     $seq->end($seq->end + $2 - 1);
 119                 }
 120                 $seq->description(sprintf("PID=%.2f LEN=%d HSP=%d",
 121                                           $pid,$hsplen,$id));
 122                 push @seqs, $seq;
 123             }
 124             $aln = new Bio::SimpleAlign();
 125             $aln->add_seq(shift @seqs);
 126             $aln->add_seq(shift @seqs);
 127
 128             $id++;
 129             $out->write_aln($aln);
 130         }
 131     }
 132 }