| blob | 51ae9942a1daa5f2763819b03369c1eb36854d9a |
1 LOCUS PX1CG 5386 bp ss-DNA circular PHG 16-JUN-2004
2 DEFINITION Coliphage phi-X174, complete genome.
3 ACCESSION J02482 M10348 M10379 M10714 M10749 M10750 M10866 M10867 M24859
4 V01128
5 VERSION J02482.1 GI:216019
6 KEYWORDS .
7 SOURCE Enterobacteria phage phiX174
8 ORGANISM Enterobacteria phage phiX174
9 Viruses; ssDNA viruses; Microviridae; Microvirus.
10 REFERENCE 1 (bases 1047 to 1094)
11 AUTHORS Ziff,E.B., Sedat,J.W. and Galibert,F.
12 TITLE Determination of the nucleotide sequence of a fragment of
13 bacteriophage phiX 174 DNA
14 JOURNAL Nature New Biol. 241 (106), 34-37 (1973)
15 PUBMED 4349156
16 REFERENCE 2 (bases 2370 to 2421)
17 AUTHORS Robertson,H.D., Barrell,B.G., Weith,H.L. and Donelson,J.E.
18 TITLE Isolation and sequence analysis of a ribosome-protected fragment
19 from bacteriophage phiX 174 DNA
20 JOURNAL Nature New Biol. 241 (106), 38-40 (1973)
21 PUBMED 4572838
22 REFERENCE 3 (bases 2370 to 2420)
23 AUTHORS Barrell,B.G., Weith,H.L., Donelson,J.E. and Robertson,H.D.
24 TITLE Sequence analysis of the ribosome-protected bacteriophase phiX174
25 DNA fragment containing the gene G initiation site
26 JOURNAL J. Mol. Biol. 92 (3), 377-393 (1975)
27 PUBMED 1095758
28 REFERENCE 4 (bases 2365 to 2591)
29 AUTHORS Air,G.M., Blackburn,E.H., Sanger,F. and Coulson,A.R.
30 TITLE The nucleotide and amino acid sequences of the N (5') terminal
31 region of gene G of bacteriophage phiphiX 174
32 JOURNAL J. Mol. Biol. 96 (4), 703-719 (1975)
33 PUBMED 1081600
34 REFERENCE 5 (bases 2263 to 2421)
35 AUTHORS Fiddes,J.C.
36 TITLE Nucleotide sequence of the intercistronic region between genes G
37 and F in bacteriophage phiX174 DNA
38 JOURNAL J. Mol. Biol. 107 (1), 1-24 (1976)
39 PUBMED 826639
40 REFERENCE 6 (bases 1017 to 1081)
41 AUTHORS Sedat,J., Ziff,E. and Galibert,F.
42 TITLE Direct determination of DNA nucleotide sequences. Structure of
43 large specific fragments of bacteriophage phiX174 DNA
44 JOURNAL J. Mol. Biol. 107 (4), 391-416 (1976)
45 PUBMED 1003475
46 REFERENCE 7 (bases 730 to 903)
47 AUTHORS Blackburn,E.H.
48 TITLE Transcription and sequence analysis of a fragment of bacteriophage
49 phiX174 DNA
50 JOURNAL J. Mol. Biol. 107 (4), 417-431 (1976)
51 PUBMED 826641
52 REFERENCE 8 (bases 1017 to 1762)
53 AUTHORS Air,G.M., Blackburn,E.H., Coulson,A.R., Galibert,F., Sanger,F.,
54 Sedat,J.W. and Ziff,E.B.
55 TITLE Gene F of bacteriophage phiX174. Correlation of nucleotide
56 sequences from the DNA and amino acid sequences from the gene
57 product
58 JOURNAL J. Mol. Biol. 107 (4), 445-458 (1976)
59 PUBMED 1088826
60 REFERENCE 9 (bases 2395 to 2922)
61 AUTHORS Air,G.M., Sanger,F. and Coulson,A.R.
62 TITLE Nucleotide and amino acid sequences of gene G of omegaX174
63 JOURNAL J. Mol. Biol. 108 (3), 519-533 (1976)
64 PUBMED 1088827
65 REFERENCE 10 (bases 4137 to 4207)
66 AUTHORS Mansfeld,A.D., Vereijken,J.M. and Jansz,H.S.
67 TITLE The nucleotide sequence of a DNA fragment, 71 base pairs in length,
68 near the origin of DNA replication of bacteriophage 0X174
69 JOURNAL Nucleic Acids Res. 3 (10), 2827-2844 (1976)
70 PUBMED 995652
71 REFERENCE 11 (bases 4505 to 5374)
72 AUTHORS Brown,N.L. and Smith,M.
73 TITLE The sequence of a region of bacteriophage phiX174 DNA coding for
74 parts of genes A and B
75 JOURNAL J. Mol. Biol. 116 (1), 1-28 (1977)
76 PUBMED 592379
77 REFERENCE 12 (bases 1 to 5375)
78 AUTHORS Sanger,F., Air,G.M., Barrell,B.G., Brown,N.L., Coulson,A.R.,
79 Fiddes,C.A., Hutchison,C.A., Slocombe,P.M. and Smith,M.
80 TITLE Nucleotide sequence of bacteriophage phi X174 DNA
81 JOURNAL Nature 265 (5596), 687-695 (1977)
82 PUBMED 870828
83 REFERENCE 13 (bases 5022 to 5132)
84 AUTHORS Brown,N.L. and Smith,M.
85 TITLE DNA sequence of a region of the phi X174 genome coding for a
86 ribosome binding site
87 JOURNAL Nature 265 (5596), 695-698 (1977)
88 PUBMED 859573
89 REFERENCE 14 (bases 5346 to 5386; 1 to 159)
90 AUTHORS Smith,M., Brown,N.L., Air,G.M., Barrell,B.G., Coulson,A.R.,
91 Hutchison,C.A. III and Sanger,F.
92 TITLE DNA sequence at the C termini of the overlapping genes A and B in
93 bacteriophage phi X174
94 JOURNAL Nature 265 (5596), 702-705 (1977)
95 PUBMED 859575
96 REFERENCE 15 (sites)
97 AUTHORS Fiddes,J.C.
98 TITLE The nucleotide sequence of a viral DNA
99 JOURNAL Sci. Am. 237 (6), 54-67 (1977)
100 PUBMED 929160
101 REFERENCE 16 (bases 1 to 5386)
102 AUTHORS Sanger,F., Coulson,A.R., Friedmann,T., Air,G.M., Barrell,B.G.,
103 Brown,N.L., Fiddes,J.C., Hutchison,C.A. III, Slocombe,P.M. and
104 Smith,M.
105 TITLE The nucleotide sequence of bacteriophage phiX174
106 JOURNAL J. Mol. Biol. 125 (2), 225-246 (1978)
107 PUBMED 731693
108 REFERENCE 17 (bases 1290 to 1302; 1340 to 1430; 1510 to 1570; 1600 to 1750)
109 AUTHORS Air,G.M., Coulson,A.R., Fiddes,J.C., Friedmann,T., Hutchison,C.A.
110 III, Sanger,F., Slocombe,P.M. and Smith,A.J.
111 TITLE Nucleotide sequence of the F protein coding region of bacteriophage
112 phiX174 and the amino acid sequence of its product
113 JOURNAL J. Mol. Biol. 125 (2), 247-254 (1978)
114 PUBMED 731694
115 REFERENCE 18 (bases 4256 to 4317)
116 AUTHORS Langeveld,S.A., van Mansfeld,A.D., de Winter,J.M. and Weisbeek,P.J.
117 TITLE Cleavage of single-stranded DNA by the A and A* proteins of
118 bacteriophage phi X174
119 JOURNAL Nucleic Acids Res. 7 (8), 2177-2188 (1979)
120 PUBMED 160544
121 REFERENCE 19 (bases 4248 to 4332)
122 AUTHORS Heidekamp,F., Langeveld,S.A., Baas,P.D. and Jansz,H.S.
123 TITLE Studies of the recognition sequence of phi X174 gene A protein.
124 Cleavage site of phi X gene A protein in St-1 RFI DNA
125 JOURNAL Nucleic Acids Res. 8 (9), 2009-2021 (1980)
126 PUBMED 6253953
127 REFERENCE 20 (bases 436 to 490; 630 to 669; 930 to 979)
128 AUTHORS Takeshita,M., Kappen,L.S., Grollman,A.P., Eisenberg,M. and
129 Goldberg,I.H.
130 TITLE Strand scission of deoxyribonucleic acid by neocarzinostatin,
131 auromomycin, and bleomycin: studies on base release and nucleotide
132 sequence specificity
133 JOURNAL Biochemistry 20 (26), 7599-7606 (1981)
134 PUBMED 6173064
135 REFERENCE 22 (bases 1064 to 1757)
136 AUTHORS Merville,M.P., Piette,J., Lopez,M., Decuyper,J. and van de Vorst,A.
137 TITLE Termination sites of the in vitro DNA synthesis on single-stranded
138 DNA photosensitized by promazines
139 JOURNAL J. Biol. Chem. 259 (24), 15069-15077 (1984)
140 PUBMED 6239864
141 REFERENCE 23 (bases 2380 to 2512; 2593 to 2786; 2788 to 2947)
142 AUTHORS Air,G.M., Els,M.C., Brown,L.E., Laver,W.G. and Webster,R.G.
143 TITLE Location of antigenic sites on the three-dimensional structure of
144 the influenza N2 virus neuraminidase
145 JOURNAL Virology 145 (2), 237-248 (1985)
146 PUBMED 2411049
147 REFERENCE 24 (bases 449 to 482; 504 to 598; 1047 to 1111)
148 AUTHORS Ueda,K., Morita,J. and Komano,T.
149 TITLE Sequence specificity of heat-labile sites in DNA induced by
150 mitomycin C
151 JOURNAL Biochemistry 23 (8), 1634-1640 (1984)
152 PUBMED 6232949
153 COMMENT On Apr 28, 2004 this sequence version replaced gi:15535.
154 [8] intermittent sequences.
155 [15] review; discussion of complete genome.
156 Double checked with sumex tape.
157 Single-stranded circular DNA which codes for eleven proteins.
158 Replicative form is duplex, icosahedron, related to s13 & g4. [21]
159 indicates that mitomycin C reduced with sodium borohydride induced
160 heat-labile sites in DNA most preferentially at dinucleotide
161 sequence 'gt' (especially 'Pu-g-t').
162 Bacteriophage phi-X174 single stranded DNA molecules were
163 irradiated with near UV light in the presence of promazine
164 derivatives, after priming with restriction fragments or synthetic
165 primers [22]. The resulting DNA fragments were used as templates
166 for in vitro complementary chain synthesis by E.coli DNA polymerase
167 I [22]. More than 90% of the observed chain terminations were
168 mapped one nucleotide before a guanine residue [22]. Photoreaction
169 occurred more predominantly with guanine residues localized in
170 single-stranded parts of the genome [22]. These same guanine
171 residues could also be damaged when the reaction was performed in
172 the dark, in the presence of promazine cation radicals [22].
173 FEATURES Location/Qualifiers
174 source 1..5386
175 /organism="Enterobacteria phage phiX174"
176 /mol_type="genomic DNA"
177 /db_xref="taxon:10847"
178 CDS join(3981..5386,1..136)
179 /function="viral strand synthesis"
180 /note="rf replication"
181 /codon_start=1
182 /transl_table=11
183 /product="A"
184 /protein_id="AAA32570.1"
185 /db_xref="GI:216020"
186 /translation="MVRSYYPSECHADYFDFERIEALKPAIEACGISTLSQSPMLGFH
187 KQMDNRIKLLEEILSFRMQGVEFDNGDMYVDGHKAASDVRDEFVSVTEKLMDELAQCY
188 NVLPQLDINNTIDHRPEGDEKWFLENEKTVTQFCRKLAAERPLKDIRDEYNYPKKKGI
189 KDECSRLLEASTMKSRRGFAIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDNPN
190 ALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLPTG
191 SVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPLKA
192 TSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNFGM
193 KMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPVTT
194 NLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKSKW
195 TAGGK"
196 CDS join(4497..5386,1..136)
197 /function="shut off host DNA synthesis"
198 /codon_start=1
199 /transl_table=11
200 /product="A*"
201 /protein_id="AAA32571.1"
202 /db_xref="GI:216021"
203 /translation="MKSRRGFAIQRLMNAMRQAHADGWFIVFDTLTLADDRLEAFYDN
204 PNALRDYFRDIGRMVLAAEGRKANDSHADCYQYFCVPEYGTANGRLHFHAVHFMRTLP
205 TGSVDPNFGRRVRNRRQLNSLQNTWPYGYSMPIAVRYTQDAFSRSGWLWPVDAKGEPL
206 KATSYMAVGFYVAKYVNKKSDMDLAAKGLGAKEWNNSLKTKLSLLPKKLFRIRMSRNF
207 GMKMLTMTNLSTECLIQLTKLGYDATPFNQILKQNAKREMRLRLGKVTVADVLAAQPV
208 TTNLLKFMRASIKMIGVSNLQSFIASMTQKLTLSDISDESKNYLDKAGITTACLRIKS
209 KWTAGGK"
210 CDS join(5075..5386,1..51)
211 /function="capsid morphogenesis"
212 /codon_start=1
213 /transl_table=11
214 /product="B"
215 /protein_id="AAA32572.1"
216 /db_xref="GI:216022"
217 /translation="MEQLTKNQAVATSQEAVQNQNEPQLRDENAHNDKSVHGVLNPTY
218 QAGLRRDAVQPDIEAERKKRDEIEAGKSYCSRRFGGATCDDKSAQIYARFDKNDWRIQ
219 PAEFYRFHDAEVNTFGYF"
220 variation 23
221 /note="in am18 and am35 [14]"
222 /replace="t"
223 variation 25
224 /note="ts116 [14]"
225 /replace="c"
226 CDS 51..221
227 /codon_start=1
228 /transl_table=11
229 /product="K"
230 /protein_id="AAA32573.1"
231 /db_xref="GI:216023"
232 /translation="MSRKIILIKQELLLLVYELNRSGLLAENEKIRPILAQLEKLLLC
233 DLSPSTNDSVKN"
234 variation 57
235 /note="am6 [14]"
236 /replace="c"
237 variation 117
238 /note="am6 [14]"
239 /replace="a"
240 CDS 133..393
241 /note="DNA maturation"
242 /codon_start=1
243 /transl_table=11
244 /product="C"
245 /protein_id="AAA32574.1"
246 /db_xref="GI:216024"
247 /translation="MRKFDLSLRSSRSSYFATFRHQLTILSKTDALDEEKWLNMLGTF
248 VKDWFRYESHFVHGRDSLVDILKERGLLSESDAVQPLIGKKS"
249 mRNA 358..3975
250 /product="major transcript"
251 mRNA 358..991
252 /product="minor transcript"
253 CDS 390..848
254 /function="capsid morphogenesis"
255 /codon_start=1
256 /transl_table=11
257 /product="D"
258 /protein_id="AAA32575.1"
259 /db_xref="GI:216025"
260 /translation="MSQVTEQSVRFQTALASIKLIQASAVLDLTEDDFDFLTSNKVWI
261 ATDRSRARRCVEACVYGTLDFVGYPRFPAPVEFIAAVIAYYVHPVNIQTACLIMEGAE
262 FTENIINGVERPVKAAELFAFTLRVRAGNTDVLTDAEENVRQKLRAEGVM"
263 CDS 568..843
264 /function="cell lysis"
265 /codon_start=1
266 /transl_table=11
267 /product="E"
268 /protein_id="AAA32576.1"
269 /db_xref="GI:216026"
270 /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNL
271 RKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVRKE"
272 CDS 848..964
273 /note="core protein; DNA condensation"
274 /codon_start=1
275 /transl_table=11
276 /product="J"
277 /protein_id="AAA32577.1"
278 /db_xref="GI:216027"
279 /translation="MSKGKKRSGARPGRPQPLRGTKGKRKGARLWYVGGQQF"
280 CDS 1001..2284
281 /note="major coat protein"
282 /codon_start=1
283 /transl_table=11
284 /product="F"
285 /protein_id="AAA32578.1"
286 /db_xref="GI:216028"
287 /translation="MSNIQTGAERMPHDLSHLGFLAGQIGRLITISTTPVIAGDSFEM
288 DAVGALRLSPLRRGLAIDSTVDIFTFYVPHRHVYGEQWIKFMKDGVNATPLPTVNTTG
289 YIDHAAFLGTINPDTNKIPKHLFQGYLNIYNNYFKAPWMPDRTEANPNELNQDDARYG
290 FRCCHLKNIWTAPLPPETELSRQMTTSTTSIDIMGLQAAYANLHTDQERDYFMQRYHD
291 VISSFGGKTSYDADNRPLLVMRSNLWASGYDVDGTDQTSLGQFSGRVQQTYKHSVPRF
292 FVPEHGTMFTLALVRFPPTATKEIQYLNAKGALTYTDIAGDPVLYGNLPPREISMKDV
293 FRSGDSSKKFKIAEGQWYRYAPSYVSPAYHLLEGFPFIQEPPSGDLQERVLIRHHDYD
294 QCFQSVQLLQWNSQVKFNVTVYRNLPTTRDSIMTS"
295 CDS 2395..2922
296 /note="major spike protein"
297 /codon_start=1
298 /transl_table=11
299 /product="G"
300 /protein_id="AAA32579.1"
301 /db_xref="GI:216029"
302 /translation="MFQTFISRHNSNFFSDKLVLTSVTPASSAPVLQTPKATSSTLYF
303 DSLTVNAGNGGFLHCIQMDTSVNAANQVVSVGADIAFDADPKFFACLVRFESSSVPTT
304 LPTAYDVYPLNGRHDGGYYTVKDCVTIDVLPRTPGNNVYVGFMVWSNFTATKCRGLVS
305 LNQVIKEIICLQPLK"
306 CDS 2931..3917
307 /function="adsorption"
308 /note="minor spike protein"
309 /codon_start=1
310 /transl_table=11
311 /product="H"
312 /protein_id="AAA32580.1"
313 /db_xref="GI:216030"
314 /translation="MFGAIAGGIASALAGGAMSKLFGGGQKAASGGIQGDVLATDNNT
315 VGMGDAGIKSAIQGSNVPNPDEAAPSFVSGAMAKAGKGLLEGTLQAGTSAVSDKLLDL
316 VGLGGKSAADKGKDTRDYLAAAFPELNAWERAGADASSAGMVDAGFENQKELTKMQLD
317 NQKEIAEMQNETQKEIAGIQSATSRQNTKDQVYAQNEMLAYQQKESTARVASIMENTN
318 LSKQQQVSEIMRQMLTQAQTAGQYFTNDQIKEMTRKVSAEVDLVHQQTQNQRYGSSHI
319 GATAKDISNVVTDAASGVVDIFHGIDKAVADTWNNFWKDGKADGIGSNLSRK"
320 misc_feature 3962
321 /note="transcription start site"
322 rep_origin 4306
323 /note="origin of viral strand synthesis"
324 misc_feature 4899
325 /note="transcription start site"
326 ORIGIN
327 1 gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa
328 61 aaattatctt gataaagcag gaattactac tgcttgttta cgaattaaat cgaagtggac
329 121 tgctggcgga aaatgagaaa attcgaccta tccttgcgca gctcgagaag ctcttacttt
330 181 gcgacctttc gccatcaact aacgattctg tcaaaaactg acgcgttgga tgaggagaag
331 241 tggcttaata tgcttggcac gttcgtcaag gactggttta gatatgagtc acattttgtt
332 301 catggtagag attctcttgt tgacatttta aaagagcgtg gattactatc tgagtccgat
333 361 gctgttcaac cactaatagg taagaaatca tgagtcaagt tactgaacaa tccgtacgtt
334 421 tccagaccgc tttggcctct attaagctca ttcaggcttc tgccgttttg gatttaaccg
335 481 aagatgattt cgattttctg acgagtaaca aagtttggat tgctactgac cgctctcgtg
336 541 ctcgtcgctg cgttgaggct tgcgtttatg gtacgctgga ctttgtggga taccctcgct
337 601 ttcctgctcc tgttgagttt attgctgccg tcattgctta ttatgttcat cccgtcaaca
338 661 ttcaaacggc ctgtctcatc atggaaggcg ctgaatttac ggaaaacatt attaatggcg
339 721 tcgagcgtcc ggttaaagcc gctgaattgt tcgcgtttac cttgcgtgta cgcgcaggaa
340 781 acactgacgt tcttactgac gcagaagaaa acgtgcgtca aaaattacgt gcggaaggag
341 841 tgatgtaatg tctaaaggta aaaaacgttc tggcgctcgc cctggtcgtc cgcagccgtt
342 901 gcgaggtact aaaggcaagc gtaaaggcgc tcgtctttgg tatgtaggtg gtcaacaatt
343 961 ttaattgcag gggcttcggc cccttacttg aggataaatt atgtctaata ttcaaactgg
344 1021 cgccgagcgt atgccgcatg acctttccca tcttggcttc cttgctggtc agattggtcg
345 1081 tcttattacc atttcaacta ctccggttat cgctggcgac tccttcgaga tggacgccgt
346 1141 tggcgctctc cgtctttctc cattgcgtcg tggccttgct attgactcta ctgtagacat
347 1201 ttttactttt tatgtccctc atcgtcacgt ttatggtgaa cagtggatta agttcatgaa
348 1261 ggatggtgtt aatgccactc ctctcccgac tgttaacact actggttata ttgaccatgc
349 1321 cgcttttctt ggcacgatta accctgatac caataaaatc cctaagcatt tgtttcaggg
350 1381 ttatttgaat atctataaca actattttaa agcgccgtgg atgcctgacc gtaccgaggc
351 1441 taaccctaat gagcttaatc aagatgatgc tcgttatggt ttccgttgct gccatctcaa
352 1501 aaacatttgg actgctccgc ttcctcctga gactgagctt tctcgccaaa tgacgacttc
353 1561 taccacatct attgacatta tgggtctgca agctgcttat gctaatttgc atactgacca
354 1621 agaacgtgat tacttcatgc agcgttacca tgatgttatt tcttcatttg gaggtaaaac
355 1681 ctcttatgac gctgacaacc gtcctttact tgtcatgcgc tctaatctct gggcatctgg
356 1741 ctatgatgtt gatggaactg accaaacgtc gttaggccag ttttctggtc gtgttcaaca
357 1801 gacctataaa cattctgtgc cgcgtttctt tgttcctgag catggcacta tgtttactct
358 1861 tgcgcttgtt cgttttccgc ctactgcgac taaagagatt cagtacctta acgctaaagg
359 1921 tgctttgact tataccgata ttgctggcga ccctgttttg tatggcaact tgccgccgcg
360 1981 tgaaatttct atgaaggatg ttttccgttc tggtgattcg tctaagaagt ttaagattgc
361 2041 tgagggtcag tggtatcgtt atgcgccttc gtatgtttct cctgcttatc accttcttga
362 2101 aggcttccca ttcattcagg aaccgccttc tggtgatttg caagaacgcg tacttattcg
363 2161 ccaccatgat tatgaccagt gtttccagtc cgttcagttg ttgcagtgga atagtcaggt
364 2221 taaatttaat gtgaccgttt atcgcaatct gccgaccact cgcgattcaa tcatgacttc
365 2281 gtgataaaag attgagtgtg aggttataac gccgaagcgg taaaaatttt aatttttgcc
366 2341 gctgaggggt tgaccaagcg aagcgcggta ggttttctgc ttaggagttt aatcatgttt
367 2401 cagactttta tttctcgcca taattcaaac tttttttctg ataagctggt tctcacttct
368 2461 gttactccag cttcttcggc acctgtttta cagacaccta aagctacatc gtcaacgtta
369 2521 tattttgata gtttgacggt taatgctggt aatggtggtt ttcttcattg cattcagatg
370 2581 gatacatctg tcaacgccgc taatcaggtt gtttctgttg gtgctgatat tgcttttgat
371 2641 gccgacccta aattttttgc ctgtttggtt cgctttgagt cttcttcggt tccgactacc
372 2701 ctcccgactg cctatgatgt ttatcctttg aatggtcgcc atgatggtgg ttattatacc
373 2761 gtcaaggact gtgtgactat tgacgtcctt ccccgtacgc cgggcaataa cgtttatgtt
374 2821 ggtttcatgg tttggtctaa ctttaccgct actaaatgcc gcggattggt ttcgctgaat
375 2881 caggttatta aagagattat ttgtctccag ccacttaagt gaggtgattt atgtttggtg
376 2941 ctattgctgg cggtattgct tctgctcttg ctggtggcgc catgtctaaa ttgtttggag
377 3001 gcggtcaaaa agccgcctcc ggtggcattc aaggtgatgt gcttgctacc gataacaata
378 3061 ctgtaggcat gggtgatgct ggtattaaat ctgccattca aggctctaat gttcctaacc
379 3121 ctgatgaggc cgcccctagt tttgtttctg gtgctatggc taaagctggt aaaggacttc
380 3181 ttgaaggtac gttgcaggct ggcacttctg ccgtttctga taagttgctt gatttggttg
381 3241 gacttggtgg caagtctgcc gctgataaag gaaaggatac tcgtgattat cttgctgctg
382 3301 catttcctga gcttaatgct tgggagcgtg ctggtgctga tgcttcctct gctggtatgg
383 3361 ttgacgccgg atttgagaat caaaaagagc ttactaaaat gcaactggac aatcagaaag
384 3421 agattgccga gatgcaaaat gagactcaaa aagagattgc tggcattcag tcggcgactt
385 3481 cacgccagaa tacgaaagac caggtatatg cacaaaatga gatgcttgct tatcaacaga
386 3541 aggagtctac tgctcgcgtt gcgtctatta tggaaaacac caatctttcc aagcaacagc
387 3601 aggtttccga gattatgcgc caaatgctta ctcaagctca aacggctggt cagtatttta
388 3661 ccaatgacca aatcaaagaa atgactcgca aggttagtgc tgaggttgac ttagttcatc
389 3721 agcaaacgca gaatcagcgg tatggctctt ctcatattgg cgctactgca aaggatattt
390 3781 ctaatgtcgt cactgatgct gcttctggtg tggttgatat ttttcatggt attgataaag
391 3841 ctgttgccga tacttggaac aatttctgga aagacggtaa agctgatggt attggctcta
392 3901 atttgtctag gaaataaccg tcaggattga caccctccca attgtatgtt ttcatgcctc
393 3961 caaatcttgg aggctttttt atggttcgtt cttattaccc ttctgaatgt cacgctgatt
394 4021 attttgactt tgagcgtatc gaggctctta aacctgctat tgaggcttgt ggcatttcta
395 4081 ctctttctca atccccaatg cttggcttcc ataagcagat ggataaccgc atcaagctct
396 4141 tggaagagat tctgtctttt cgtatgcagg gcgttgagtt cgataatggt gatatgtatg
397 4201 ttgacggcca taaggctgct tctgacgttc gtgatgagtt tgtatctgtt actgagaagt
398 4261 taatggatga attggcacaa tgctacaatg tgctccccca acttgatatt aataacacta
399 4321 tagaccaccg ccccgaaggg gacgaaaaat ggtttttaga gaacgagaag acggttacgc
400 4381 agttttgccg caagctggct gctgaacgcc ctcttaagga tattcgcgat gagtataatt
401 4441 accccaaaaa gaaaggtatt aaggatgagt gttcaagatt gctggaggcc tccactatga
402 4501 aatcgcgtag aggctttgct attcagcgtt tgatgaatgc aatgcgacag gctcatgctg
403 4561 atggttggtt tatcgttttt gacactctca cgttggctga cgaccgatta gaggcgtttt
404 4621 atgataatcc caatgctttg cgtgactatt ttcgtgatat tggtcgtatg gttcttgctg
405 4681 ccgagggtcg caaggctaat gattcacacg ccgactgcta tcagtatttt tgtgtgcctg
406 4741 agtatggtac agctaatggc cgtcttcatt tccatgcggt gcactttatg cggacacttc
407 4801 ctacaggtag cgttgaccct aattttggtc gtcgggtacg caatcgccgc cagttaaata
408 4861 gcttgcaaaa tacgtggcct tatggttaca gtatgcccat cgcagttcgc tacacgcagg
409 4921 acgctttttc acgttctggt tggttgtggc ctgttgatgc taaaggtgag ccgcttaaag
410 4981 ctaccagtta tatggctgtt ggtttctatg tggctaaata cgttaacaaa aagtcagata
411 5041 tggaccttgc tgctaaaggt ctaggagcta aagaatggaa caactcacta aaaaccaagc
412 5101 tgtcgctact tcccaagaag ctgttcagaa tcagaatgag ccgcaacttc gggatgaaaa
413 5161 tgctcacaat gacaaatctg tccacggagt gcttaatcca acttaccaag ctgggttacg
414 5221 acgcgacgcc gttcaaccag atattgaagc agaacgcaaa aagagagatg agattgaggc
415 5281 tgggaaaagt tactgtagcc gacgttttgg cggcgcaacc tgtgacgaca aatctgctca
416 5341 aatttatgcg cgcttcgata aaaatgattg gcgtatccaa cctgca
417 //