t/data/primer3_outfile.txt

   1 PRIMER_SEQUENCE_ID=sN11902
   2 PRIMER_COMMENT=3831
   3 SEQUENCE=ACAGCAACATCGAACTCGTCGTCTTCGAAAGCCTCTTTGGCCTTCGCTGCAAGCTTCTTAGCCATGTCGATTGTGGTTTTTGGTTAGGTTGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGATGATTCGATTCAAGAAAGGGAATGAAGTAGTAGACGAGGGCCAAAGAGTATAAAGATGGATGCTATCTTTTTCTCTTCTATTGTTCTGTTATTGTTTTGTGAGGGAAGTTTTGGCGCTTCAATACAAAAAAATAAATAAAGAATAAACAAATACAGCTTAGTTAAATGGGTATAACATGGGCCGGAATATATAATCACGAATTAAGGCCCATCATTGTCTCAAAATTAAACTCGGCCTCTATAATACTTCTCCCACACCGGTAATCTTGAGATGAACACTCCTCTTTCGTGTAGTATAAAATAAGACGAAGGTCCAACGTTGAAAGATACTTACCTGGACGGGGTCTAGTCCACGCGTGGACTAAACTTGAGGACCAACGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAGTCGTAAAGTGGAAGCAGCGATGAAGAGTAACGATAGTAGTAAGAACAGAGTGAGTTGGTTTATAGATGAAGATCCGTCTCCACGTCCTCCGAAATGCACAGTTCTTTGGAAAGAGCTTTTGCGTTTGAAGAAACAGAGGAGCTCTTCGTCGGTGAAGTCCTCTCTGTCTCCGTCGTCGTCCACGTCATCATCTAGCTCGCTTGAGGATAAGAGAGAAGGGAAGAGAGGTAAGAAAGGATTAGAGAGGACGAGATCGTC
   4 TARGET=513,26
   5 PRIMER_PRODUCT_SIZE_RANGE=100-500
   6 PRIMER_FILE_FLAG=0
   7 PRIMER_LIBERAL_BASE=1
   8 PRIMER_NUM_RETURN=1
   9 PRIMER_FIRST_BASE_INDEX=1
  10 PRIMER_EXPLAIN_FLAG=1
  11 PRIMER_LEFT_EXPLAIN=considered 4849, GC content failed 207, low tm 2062, high tm 1173, long poly-x seq 11, ok 1396
  12 PRIMER_RIGHT_EXPLAIN=considered 2415, low tm 892, high tm 844, high end compl 3, ok 676
  13 PRIMER_PAIR_EXPLAIN=considered 2, high end compl 1, ok 1
  14 PRIMER_PAIR_PENALTY=0.0991
  15 PRIMER_LEFT_PENALTY=0.095506
  16 PRIMER_RIGHT_PENALTY=0.003589
  17 PRIMER_LEFT_SEQUENCE=CACGCGTGGACTAAACTTGA
  18 PRIMER_RIGHT_SEQUENCE=AACGCAAAAGCTCTTTCCAA
  19 PRIMER_LEFT=484,20
  20 PRIMER_RIGHT=668,20
  21 PRIMER_LEFT_TM=59.904
  22 PRIMER_RIGHT_TM=59.996
  23 PRIMER_LEFT_GC_PERCENT=50.000
  24 PRIMER_RIGHT_GC_PERCENT=40.000
  25 PRIMER_LEFT_SELF_ANY=8.00
  26 PRIMER_RIGHT_SELF_ANY=6.00
  27 PRIMER_LEFT_SELF_END=3.00
  28 PRIMER_RIGHT_SELF_END=2.00
  29 PRIMER_LEFT_END_STABILITY=7.0000
  30 PRIMER_RIGHT_END_STABILITY=8.5000
  31 PRIMER_PAIR_COMPL_ANY=4.00
  32 PRIMER_PAIR_COMPL_END=2.00
  33 PRIMER_PRODUCT_SIZE=185
  34 =
  35 PRIMER_SEQUENCE_ID=sN11926
  36 PRIMER_COMMENT=3854
  37 SEQUENCE=ACGCTGCTCGGAGAAGCGGCGCCGATATCAATCCGTCAGAAAATGTTTGTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTCGATCTTTGTGTGTGTGTTTCTTTTAGATCTGACTTTGTAGTTTTAGGTTTGATTGGGCGATTGAATCGAGATGAGTTTTGTTCATTTAAGTTTCAGTTGCCGACACTGACGTAGAGATTTGTGTTAGGGTCCTCGTTTGTTGTGAATTTCAATTGACAAATCTGTTTTGGACATCTAACAAAGGGCTTTGTTTTTGTTTTTGTTGCATATGTAGTCAATGCTGGAACCAACAAAGCGGCTTCGAGCAGCACCTCCTTGAACACAAAGAGGCTCGATGATGATACTGAGACCTTAGCTCGTGAGTATATATAATTCATCTATTCTCTCATTCACAGCGAATTAGTATCGACTTCTATATCTCTCTGTGGTTCAAATAAACTTTAGTTGTATCTTCAAAGCTTTATATACTGTTTTTCTTTCCCGTGTTTAAGAGATTGATTAGTATATTGCTTGTTCGAAGAGTCTTTGTT
  38 TARGET=54,23
  39 PRIMER_PRODUCT_SIZE_RANGE=100-500
  40 PRIMER_FILE_FLAG=0
  41 PRIMER_LIBERAL_BASE=1
  42 PRIMER_NUM_RETURN=1
  43 PRIMER_FIRST_BASE_INDEX=1
  44 PRIMER_EXPLAIN_FLAG=1
  45 PRIMER_LEFT_EXPLAIN=considered 315, low tm 52, high tm 212, ok 51
  46 PRIMER_RIGHT_EXPLAIN=considered 4504, GC content failed 63, low tm 2423, high tm 832, ok 1186
  47 PRIMER_PAIR_EXPLAIN=considered 1, ok 1
  48 PRIMER_PAIR_PENALTY=1.0219
  49 PRIMER_LEFT_PENALTY=0.973324
  50 PRIMER_RIGHT_PENALTY=0.048588
  51 PRIMER_LEFT_SEQUENCE=GCCGATATCAATCCGTCAGA
  52 PRIMER_RIGHT_SEQUENCE=CTCTACGTCAGTGTCGGCAA
  53 PRIMER_LEFT=21,20
  54 PRIMER_RIGHT=196,20
  55 PRIMER_LEFT_TM=60.973
  56 PRIMER_RIGHT_TM=60.049
  57 PRIMER_LEFT_GC_PERCENT=50.000
  58 PRIMER_RIGHT_GC_PERCENT=55.000
  59 PRIMER_LEFT_SELF_ANY=6.00
  60 PRIMER_RIGHT_SELF_ANY=5.00
  61 PRIMER_LEFT_SELF_END=3.00
  62 PRIMER_RIGHT_SELF_END=2.00
  63 PRIMER_LEFT_END_STABILITY=6.7000
  64 PRIMER_RIGHT_END_STABILITY=10.0000
  65 PRIMER_PAIR_COMPL_ANY=5.00
  66 PRIMER_PAIR_COMPL_END=3.00
  67 PRIMER_PRODUCT_SIZE=176
  68 =
  69 PRIMER_SEQUENCE_ID=sN1922
  70 PRIMER_COMMENT=3922
  71 SEQUENCE=ACAGCAACATCGAACTCGTCGTCTTCGAAAGCCTCTTTGGCCTTCGCTGCAAGCTTCTTAGCCATGTCGATTGTGGTTTTTGGTTAGGTTGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGATGATTCGATTCAAGAAAGGGAATGAAGTAGTAGACGAGGGCCAAAGAGTATAAAGATGGATGCGATCTTTTTCTCTTCTATTGTTCTGTTATTGTTTTGTGAGGGAAGTTTTGGCGCTTCAATACAAAAAAATAAATAAAGAATAAACAAATACAGCTTAGTTAAATGGGTATAACATGGGCCGGAATATATAATCACGGATTAAGGCCCATCATTGTCTCAAAATTAAACTCGGCCTCTATAATACTTCTCCCACACCGGTAATCTTGAGATGAACACTCCTCTTTCGTGTAGTATAAAATAAGACGAAGGTCCAACGTTGAAAGATACTTACCTGGACGGGGTC
  72 TARGET=96,32
  73 PRIMER_PRODUCT_SIZE_RANGE=100-500
  74 PRIMER_FILE_FLAG=0
  75 PRIMER_LIBERAL_BASE=1
  76 PRIMER_NUM_RETURN=1
  77 PRIMER_FIRST_BASE_INDEX=1
  78 PRIMER_EXPLAIN_FLAG=1
  79 PRIMER_LEFT_EXPLAIN=considered 735, low tm 100, high tm 441, ok 194
  80 PRIMER_RIGHT_EXPLAIN=considered 3232, GC content failed 191, low tm 1479, high tm 573, long poly-x seq 12, ok 977
  81 PRIMER_PAIR_EXPLAIN=considered 3, high end compl 1, ok 2
  82 PRIMER_PAIR_PENALTY=0.2733
  83 PRIMER_LEFT_PENALTY=0.124828
  84 PRIMER_RIGHT_PENALTY=0.148509
  85 PRIMER_LEFT_SEQUENCE=CGTCTTCGAAAGCCTCTTTG
  86 PRIMER_RIGHT_SEQUENCE=CAACGTTGGACCTTCGTCTT
  87 PRIMER_LEFT=20,20
  88 PRIMER_RIGHT=454,20
  89 PRIMER_LEFT_TM=60.125
  90 PRIMER_RIGHT_TM=60.149
  91 PRIMER_LEFT_GC_PERCENT=50.000
  92 PRIMER_RIGHT_GC_PERCENT=50.000
  93 PRIMER_LEFT_SELF_ANY=6.00
  94 PRIMER_RIGHT_SELF_ANY=8.00
  95 PRIMER_LEFT_SELF_END=3.00
  96 PRIMER_RIGHT_SELF_END=1.00
  97 PRIMER_LEFT_END_STABILITY=7.3000
  98 PRIMER_RIGHT_END_STABILITY=6.4000
  99 PRIMER_PAIR_COMPL_ANY=4.00
 100 PRIMER_PAIR_COMPL_END=0.00
 101 PRIMER_PRODUCT_SIZE=435
 102 =
 103