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1 LOCUS       MSGEFTUA                1500 bp    DNA     linear   BCT 18-JUN-1999
2 DEFINITION  Mycobacterium leprae Tuf gene for elongation factor Tu, complete
3             cds.
4 ACCESSION   D13869
5 VERSION     D13869.1  GI:434782
6 KEYWORDS    EF-Tu; elongation factor Tu.
7 SOURCE      Mycobacterium leprae
8   ORGANISM  Mycobacterium leprae
9             Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
10             Corynebacterineae; Mycobacteriaceae; Mycobacterium.
11 REFERENCE   1  (bases 1 to 1500)
12   AUTHORS   Dhandayuthapani,S., Banu,M.J. and Kashiwabara,Y.
13   TITLE     Cloning and sequence determination of the gene coding for the
14             elongation factor Tu of Mycobacterium leprae
15   JOURNAL   J. Biochem. 115 (4), 664-669 (1994)
16    PUBMED   8089081
17 COMMENT     Submitted (07-DEC-1992) to DDBJ by:
18             Subranamian Dhandayuthapani
19             National Institute for Leprosy Research
20             4-2-1 Aobacho, Higashimurayama-shi
21             Tokyo 189
22             Japan
23             Phone:  0423-91-8211
24             Fax:    0423-94-9092.
25 FEATURES             Location/Qualifiers
26      source          1..1500
27                      /organism="Mycobacterium leprae"
28                      /mol_type="genomic DNA"
29                      /isolate="Thai 53"
30                      /db_xref="taxon:1769"
31                      /clone_lib="lambda gt11"
32      repeat_region   20..71
33                      /rpt_type=direct
34                      /rpt_unit="20..29"
35                      /rpt_unit="62..71"
36      repeat_region   90..231
37                      /rpt_type=direct
38                      /rpt_unit="90..99"
39                      /rpt_unit="222..231"
40      RBS             205..210
41                      /standard_name="Shine-Dalgarno sequence"
42      gene            219..1409
43                      /gene="Tuf"
44      CDS             219..1409
45                      /gene="Tuf"
46                      /function="peptide chain elongtion during protein
47                      synthesis"
48                      /standard_name="EF-Tu"
49                      /note="start codon gtg"
50                      /codon_start=1
51                      /evidence=not_experimental
52                      /transl_table=11
53                      /product="elongation factor Tu"
54                      /protein_id="BAA02982.2"
55                      /db_xref="GI:5103687"
56                      /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPNLN
57                      ESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMD
58                      GAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDAVDDEELLELVEMEVRELL
59                      AAQEFDEDAPVVRVSALKALEGDAKWVESVTQLMDAVDESIPAPVRETDKPFLMPVED
60                      VFTITGRGTVVTGRVERGVVNVNEEVEIVGIRQTTTKTTVTGVEMFRKLLDQGQAGDN
61                      VGLLLRGIKREDVERGQVVIKPGTTTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFY
62                      FRTTDVTGVVTLPEGTEMVMPGDNTNISVTLIQPVAMDEGLRFAIREGGPTVGAGRVV
63                      KIIK"
64      repeat_region   465..729
65                      /rpt_type=direct
66                      /rpt_unit="465..474"
67                      /rpt_unit="720..729"
68      repeat_region   883..913
69                      /rpt_type=direct
70                      /rpt_unit="883..892"
71                      /rpt_unit="904..913"
72      repeat_region   1169..1369
73                      /rpt_type=direct
74                      /rpt_unit="1169..1180"
75                      /rpt_unit="1358..1369"
76 ORIGIN      
77         1 gctcggtcca agacgcaagg ccggcgaact actccatggt gttcaactcg tactccgaag
78        61 tgccggcgaa cgtgtcgaag gagatcatcg cgaaggcgac gggcgagtag gagttgagtg
79       121 gtcggaaaag cctacgagtt atgctgacgc ggtcactacc gcggataaat tactaacatt
80       181 gctttttaca agcaccaata gtccaggagg acacagaagt ggcgaaggcg aagttcgagc
81       241 ggacgaagcc gcacgtcaac atcgggacca tcggtcacgt tgaccacggc aagaccacat
82       301 tgaccgcggc tattaccaag gtcctgcatg acaagttccc caacctgaat gagtcgcgcg
83       361 cctttgacca gattgacaac gcgcccgagg agcgtcagcg cggtatcacc atcaacattt
84       421 cccacgtgga gtatcagacc gagaagcgtc actatgctca cgtcgacgcc ccggggcacg
85       481 ccgactacat taagaacatg atcaccggtg cggcccagat ggatggtgcg attctggtgg
86       541 tcgctgctac agacggcccg atgccgcaga ctcgcgagca cgtgctgctc gctcgtcagg
87       601 tgggtgtacc ttacatcctg gtcgcactta acaagtccga cgccgtggac gacgaggaac
88       661 tactcgagct tgtcgagatg gaagtccgtg agttgctggc tgcccaggaa ttcgacgagg
89       721 acgccccggt tgtgcgtgtc tcggcattga aggcgctcga gggtgacgcc aagtgggtcg
90       781 agtctgtcac acagttgatg gacgctgtcg acgagtcgat cccggctcca gtccgcgaga
91       841 ctgacaagcc gttcctgatg cccgttgagg acgtcttcac tatcaccggt cgtggcaccg
92       901 tggtcaccgg tcgggtggag cgcggcgtgg tcaacgtgaa cgaggaagtt gagatcgtcg
93       961 gcattcgtca gacgaccacc aagaccaccg tcacgggtgt ggaaatgttt cgtaagctgc
94      1021 tcgaccaagg tcaggccggt gataacgttg gtctgttgtt gcgtggcatc aagcgcgagg
95      1081 acgtcgagcg tggtcaggtg gtcatcaaac ccggcaccac caccccgcat accgagttcg
96      1141 aaggccaagt atacatcctg tccaaggacg aaggtggtcg gcacacaccg ttcttcaaca
97      1201 actaccgtcc gcagttctac ttccgtacca ctgatgtgac cggtgtggtg actttgccgg
98      1261 agggcaccga gatggtgatg ccaggtgaca acaccaacat ctcggtgacg ttgattcagc
99      1321 ccgttgccat ggacgagggt ttgcgcttcg cgatccgcga aggtggtccg actgttggtg
100      1381 ccggccgggt cgtcaagatc atcaagtaag tcgcatcaac gggccaggtg gttacaacgg
101      1441 tccacatttg cgaaacggcg ctcatccgaa aggataggcg cgtttctttt gagtttatgc