1 BLASTX 2.0MP-WashU [12-Feb-2001] [linux-i686 01:36:08 31-Jan-2001]
3 Copyright (C) 1996-2000 Washington University, Saint Louis, Missouri USA.
6 Reference: Gish, W. (1996-2000) http://blast.wustl.edu
7 Gish, Warren and David J. States (1993). Identification of protein coding
8 regions by database similarity search. Nat. Genet. 3:266-72.
10 Notice: statistical significance is estimated under the assumption that the
11 equivalent of one entire reading frame in the query sequence codes for protein
12 and that significant alignments will involve only coding reading frames.
14 Query= gi|142864|gb|M10040.1|BACDNAE B.subtilis dnaE gene encoding DNA
18 Translating both strands of query sequence in all 6 reading frames
21 4289 sequences; 1,358,990 total letters.
22 Searching....10....20....30....40....50....60....70....80....90....100% done
27 Sequences producing High-scoring Segment Pairs: Score P(N) N
29 gi|1789447|gb|AAC76102.1| (AE000388) DNA biosynthesis; DN... 671 1.1e-74 1
33 >gi|1789447|gb|AAC76102.1| (AE000388) DNA biosynthesis; DNA primase
39 Score = 671 (265.8 bits), Expect = 1.1e-74, P = 1.1e-74
40 Identities = 151/421 (35%), Positives = 223/421 (52%), Frame = +3
42 Query: 21 MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFH 200
43 M RIP ++ + DIV++I V+LKKQG+N+ CPFH E TPSF+V+ +KQ +H
44 Sbjct: 1 MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYH 60
46 Query: 201 CFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARP---ESSGEQKM 371
47 CFGCGA GN FL + F E+V LA + ++ P + +G+ P E Q +
48 Sbjct: 61 CFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFE----AGSGPSQIERHQRQTL 116
50 Query: 372 AEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKR 551
51 + + L FY L A YL RG + E+I F IG+A WD + K
52 Sbjct: 117 YQLMDGLNTFYQQSL-QQPVATSARQYLEKRGLSHEVIARFAIGFAPPGWDNVLKRFGGN 175
54 Query: 552 GFSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSP 731
55 + + AG+L+ + G Y DRFR RVMFPI D G V+ F GR LG+ PKY+NSP
56 Sbjct: 176 PENRQSLIDAGMLVTNDQGRSY-DRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSP 234
58 Query: 732 ETPLFHKSKLLYNFYKARLHIRKQERAVLFEGFADVYTAVSSDVKESIATMGTSLTDDHV 911
59 ET +FHK + LY Y+A+ + R ++ EG+ DV + ++A++GTS T DH+
60 Sbjct: 235 ETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHI 294
62 Query: 912 KILRRNVEEIILCYDSDKAGYEATLKASELL---QKKGCKVRVAMIPDGLDPDDYIKKFG 1082
63 ++L R +I CYD D+AG +A +A E G ++R +PDG DPD ++K G
64 Sbjct: 295 QLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEG 354
66 Query: 1083 GEKFKNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVK 1262
67 E F+ + + ++ + AF +LS R L IS + G + +Y++
68 Sbjct: 355 KEAFEARM-EQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLISQVPGETLR-IYLR 412
98 Query ----- As Used ----- ----- Computed ----
99 Frame MatID Matrix name Lambda K H Lambda K H
100 +3 0 blosum62 0.318 0.135 0.401 0.324 0.139 0.405
101 Q=12,R=1 0.270 0.0470 0.230 n/a n/a n/a
102 +2 0 blosum62 0.318 0.135 0.401 0.365 0.163 0.618
103 Q=12,R=1 0.270 0.0470 0.230 n/a n/a n/a
104 +1 0 blosum62 0.318 0.135 0.401 0.356 0.155 0.528
105 Q=12,R=1 0.270 0.0470 0.230 n/a n/a n/a
106 -1 0 blosum62 0.318 0.135 0.401 0.350 0.155 0.543
107 Q=12,R=1 0.270 0.0470 0.230 n/a n/a n/a
108 -2 0 blosum62 0.318 0.135 0.401 0.350 0.155 0.505
109 Q=12,R=1 0.270 0.0470 0.230 n/a n/a n/a
110 -3 0 blosum62 0.318 0.135 0.401 0.358 0.157 0.543
111 Q=12,R=1 0.270 0.0470 0.230 n/a n/a n/a
114 Frame MatID Length Eff.Length E S W T X E2 S2
115 +3 0 666 666 10. 59 3 12 16 0.021 41
117 +2 0 666 666 10. 59 3 12 16 0.021 41
119 +1 0 667 667 10. 59 3 12 16 0.021 41
121 -1 0 667 667 10. 59 3 12 16 0.021 41
123 -2 0 666 666 10. 59 3 12 16 0.021 41
125 -3 0 666 666 10. 59 3 12 16 0.021 41
131 Database: /home/jes12/db/ecoli.aa
133 Posted: 2:52:35 PM EST Nov 18, 2001
134 Created: 9:46:47 AM EST Nov 18, 2001
136 # of letters in database: 1,358,990
137 # of sequences in database: 4289
138 # of database sequences satisfying E: 1
139 No. of states in DFA: 600 (64 KB)
140 Total size of DFA: 655 KB (1283 KB)
141 Time to generate neighborhood: 0.04u 0.01s 0.05t Elapsed: 00:00:00
142 No. of threads or processors used: 1
143 Search cpu time: 0.44u 0.01s 0.45t Elapsed: 00:00:01
144 Total cpu time: 0.48u 0.02s 0.50t Elapsed: 00:00:01
145 Start: Sat Apr 20 14:39:05 2002 End: Sat Apr 20 14:39:06 2002