4 CXGN::Cross - an object representing a Cross in the database
8 my $cross = CXGN::Cross->new( { schema => $schema, cross_stock_id => 37347 });
10 my $cross_name = $cross->cross_name(); # get cross name
11 my $female_parent = $cross->female_parent(); #name of female parent
12 my $female_parent_id = $cross->female_parent_id(); # id of female parent
13 my $male_parent = $cross->male_parent(); # etc.
14 my $progenies = $cross->progenies();
31 use SGN
::Model
::Cvterm
;
35 extends
'CXGN::Stock';
37 has
'cross_stock_id' => (isa
=> "Maybe[Int]",
42 has
'cross_name' => (isa
=> 'Maybe[Str]',
46 has
'female_parent' => (isa
=> 'Str',
51 has
'female_parent_id' => (isa
=> 'Int',
55 has
'male_parent' => (isa
=> 'Str',
60 has
'male_parent_id' => (isa
=> 'Int',
64 has
'trial_id' => (isa
=> "Int",
69 has
'progenies' => (isa
=> 'Ref',
78 my $schema = $args->{schema
};
79 my $cross_id = $args->{cross_stock_id
};
81 $self->stock_id($cross_id);
83 my $female_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_parent', 'stock_relationship')->cvterm_id();
84 my $male_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_parent', 'stock_relationship')->cvterm_id();
85 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross', 'stock_type')->cvterm_id();
87 my $row = $schema->resultset("Stock::Stock")->find( { stock_id
=> $cross_id, type_id
=> $cross_type_id });
90 my $name = $row->uniquename();
92 $self->cross_name($name);
93 $self->cross_stock_id($cross_id);
97 # to do: populate female_parent, male_parent etc.
98 my ($female_parent, $male_parent, @progenies) = $self->get_cross_relationships();
99 print STDERR Dumper
($female_parent);
101 if (ref($female_parent)) {
102 $self->female_parent($female_parent->[0]);
103 $self->female_parent_id($female_parent->[1]);
105 if (ref($male_parent)) {
106 $self->male_parent($male_parent->[0]);
107 $self->male_parent_id($male_parent->[1]);
110 $self->progenies(\
@progenies);
114 sub get_cross_relationships
{
117 my $crs = $self->schema->resultset("Stock::StockRelationship")->search( { object_id
=> $self->cross_stock_id } );
119 my $maternal_parent = "";
120 my $paternal_parent = "";
123 foreach my $child ($crs->all()) {
124 if ($child->type->name() eq "female_parent") {
125 $maternal_parent = [ $child->subject->name, $child->subject->stock_id() ];
127 if ($child->type->name() eq "male_parent") {
128 $paternal_parent = [ $child->subject->name, $child->subject->stock_id() ];
130 if ($child->type->name() eq "offspring_of") {
131 my $is_progeny_obsolete = $child->subject->is_obsolete();
132 if ($is_progeny_obsolete == 0 ){
133 push @progeny, [ $child->subject->name, $child->subject->stock_id() ]
138 return ($maternal_parent, $paternal_parent, \
@progeny);
144 my $schema = $self->schema;
145 my $cross_id = $self->cross_stock_id;
147 my $cross_member_of_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_member_of", "stock_relationship")->cvterm_id();
148 my $cross_experiment_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross_experiment', 'experiment_type')->cvterm_id();
149 my $family_name_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "family_name", "stock_type")->cvterm_id();
151 my $q = "SELECT project.project_id, project.name, project.description, stock.stock_id, stock.uniquename
152 FROM nd_experiment_stock
153 JOIN nd_experiment ON (nd_experiment_stock.nd_experiment_id = nd_experiment.nd_experiment_id) AND nd_experiment.type_id = ?
154 JOIN nd_experiment_project ON (nd_experiment_project.nd_experiment_id = nd_experiment.nd_experiment_id)
155 JOIN project ON (nd_experiment_project.project_id = project.project_id)
156 LEFT JOIN stock_relationship ON (nd_experiment_stock.stock_id = stock_relationship.subject_id) AND stock_relationship.type_id = ?
157 LEFT JOIN stock ON (stock_relationship.object_id = stock.stock_id) AND stock.type_id = ?
158 WHERE nd_experiment_stock.stock_id = ?";
160 my $h = $schema->storage->dbh()->prepare($q);
161 $h->execute($cross_experiment_type_id, $cross_member_of_type_id, $family_name_type_id, $cross_id);
163 my @membership_info = ();
164 while (my ($crossing_experiment_id, $crossing_experiment_name, $description, $family_id, $family_name) = $h->fetchrow_array()){
165 push @membership_info, [$crossing_experiment_id, $crossing_experiment_name, $description, $family_id, $family_name]
168 return \
@membership_info;
174 Usage: CXGN::Cross->cross_parents($schema, $cross_id);
185 my $schema = $self->schema;
186 my $cross_id = $self->cross_stock_id;
188 my $female_accession_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_parent', 'stock_relationship')->cvterm_id();
189 my $female_plot_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_plot_of', 'stock_relationship')->cvterm_id();
190 my $male_accession_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_parent', 'stock_relationship')->cvterm_id();
191 my $male_plot_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_plot_of', 'stock_relationship')->cvterm_id();
192 my $female_plant_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_plant_of', 'stock_relationship')->cvterm_id();
193 my $male_plant_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_plant_of', 'stock_relationship')->cvterm_id();
194 my $cross_combination_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross_combination', 'stock_property')->cvterm_id();
195 my $ploidy_level_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'ploidy_level', 'stock_property')->cvterm_id();
197 my $q ="SELECT female_accession.stock_id, female_accession.uniquename, female_plot.stock_id, female_plot.uniquename, female_plant.stock_id, female_plant.uniquename,
198 male_accession.stock_id, male_accession.uniquename, male_plot.stock_id, male_plot.uniquename, male_plant.stock_id, male_plant.uniquename,
199 female_relationship.value, cross_combination.value, female_ploidy.value, male_ploidy.value
201 JOIN stock_relationship AS female_relationship ON (stock.stock_id = female_relationship.object_id) AND female_relationship.type_id = ?
202 JOIN stock AS female_accession ON (female_relationship.subject_id = female_accession.stock_id)
203 LEFT JOIN stockprop AS female_ploidy ON (female_accession.stock_id = female_ploidy.stock_id) AND female_ploidy.type_id = ?
204 LEFT JOIN stock_relationship AS female_plot_relationship ON (stock.stock_id = female_plot_relationship.object_id) AND female_plot_relationship.type_id = ?
205 LEFT JOIN stock AS female_plot on (female_plot_relationship.subject_id = female_plot.stock_id)
206 LEFT JOIN stock_relationship AS female_plant_relationship ON (stock.stock_id = female_plant_relationship.object_id) AND female_plant_relationship.type_id = ?
207 LEFT JOIN stock AS female_plant ON (female_plant_relationship.subject_id = female_plant.stock_id)
208 LEFT JOIN stock_relationship AS male_relationship ON (stock.stock_id = male_relationship.object_id) AND male_relationship.type_id = ?
209 LEFT JOIN stock AS male_accession ON (male_relationship.subject_id = male_accession.stock_id)
210 LEFT JOIN stockprop AS male_ploidy ON (male_accession.stock_id = male_ploidy.stock_id) AND male_ploidy.type_id = ?
211 LEFT JOIN stock_relationship AS male_plot_relationship ON (stock.stock_id = male_plot_relationship.object_id) AND male_plot_relationship.type_id = ?
212 LEFT JOIN stock AS male_plot ON (male_plot_relationship.subject_id = male_plot.stock_id)
213 LEFT JOIN stock_relationship AS male_plant_relationship ON (stock.stock_id = male_plant_relationship.object_id) AND male_plant_relationship.type_id = ?
214 LEFT JOIN stock AS male_plant ON (male_plant_relationship.subject_id =male_plant.stock_id)
215 LEFT JOIN stockprop AS cross_combination ON (stock.stock_id = cross_combination.stock_id) AND cross_combination.type_id =?
216 WHERE stock.stock_id = ?";
218 my $h = $schema->storage->dbh()->prepare($q);
219 $h->execute($female_accession_cvterm, $ploidy_level_cvterm, $female_plot_cvterm, $female_plant_cvterm, $male_accession_cvterm, $ploidy_level_cvterm, $male_plot_cvterm, $male_plant_cvterm, $cross_combination_cvterm, $cross_id);
221 my @cross_parents = ();
222 while(my ($female_accession_id, $female_accession_name, $female_plot_id, $female_plot_name, $female_plant_id, $female_plant_name, $male_accession_id, $male_accession_name, $male_plot_id, $male_plot_name, $male_plant_id, $male_plant_name, $cross_type, $cross_combination, $female_ploidy, $male_ploidy) = $h->fetchrow_array()){
223 push @cross_parents, [$female_accession_id, $female_accession_name, $female_plot_id, $female_plot_name, $female_plant_id, $female_plant_name, $male_accession_id, $male_accession_name, $male_plot_id, $male_plot_name, $male_plant_id, $male_plant_name, $cross_type, $cross_combination, $female_ploidy, $male_ploidy]
226 return \
@cross_parents;
231 =head2 get_cross_details
233 Usage: CXGN::Cross->get_cross_details( $schema, $female_parent, $male_parent);
242 sub get_cross_details
{
245 my $female_parent = shift;
246 my $male_parent = shift;
248 my $male_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_parent", "stock_relationship")->cvterm_id();
249 my $female_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_parent", "stock_relationship")->cvterm_id();
250 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross", "stock_type")->cvterm_id();
251 my $family_name_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "family_name", "stock_type")->cvterm_id();
252 my $member_of_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "member_of", "stock_relationship")->cvterm_id();
253 my $cross_experiment_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross_experiment', 'experiment_type')->cvterm_id();
255 my $where_clause = "";
256 if ($female_parent && $male_parent) {
257 $where_clause = "WHERE female_parent.uniquename = ? AND male_parent.uniquename = ? ORDER BY stock_relationship1.value";
259 elsif ($female_parent) {
260 $where_clause = "WHERE female_parent.uniquename = ? ORDER BY male_parent.uniquename, stock_relationship1.value";
262 elsif ($male_parent) {
263 $where_clause = "WHERE male_parent.uniquename = ? ORDER BY female_parent.uniquename, stock_relationship1.value";
266 my $q = "SELECT female_parent.stock_id, male_parent.stock_id, cross_entry.stock_id, female_parent.uniquename, male_parent.uniquename, cross_entry.uniquename, stock_relationship1.value, family.stock_id, family.uniquename, project.project_id, project.name
267 FROM stock as female_parent INNER JOIN stock_relationship AS stock_relationship1 ON (female_parent.stock_id = stock_relationship1.subject_id) AND stock_relationship1.type_id = ?
268 INNER JOIN stock AS cross_entry ON (cross_entry.stock_id = stock_relationship1.object_id) AND cross_entry.type_id= ?
269 LEFT JOIN stock_relationship AS stock_relationship2 ON (cross_entry.stock_id = stock_relationship2.object_id) AND stock_relationship2.type_id = ?
270 LEFT JOIN stock AS male_parent ON (male_parent.stock_id = stock_relationship2.subject_id)
271 LEFT JOIN stock_relationship AS stock_relationship3 ON (stock_relationship3.subject_id = cross_entry.stock_id) AND stock_relationship3.type_id = ?
272 LEFT JOIN stock AS family ON (stock_relationship3.object_id = family.stock_id) AND family.type_id = ?
273 LEFT JOIN nd_experiment_stock ON (nd_experiment_stock.stock_id = cross_entry.stock_id)
274 LEFT JOIN nd_experiment_project ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id) AND nd_experiment_stock.type_id = ?
275 LEFT JOIN project ON (nd_experiment_project.project_id = project.project_id)
278 my $h = $schema->storage->dbh()->prepare($q);
280 if ($female_parent && $male_parent) {
281 $h->execute($female_parent_type_id, $cross_type_id, $male_parent_type_id, $member_of_type_id, $family_name_type_id, $cross_experiment_cvterm, $female_parent, $male_parent);
283 elsif ($female_parent) {
284 $h->execute($female_parent_type_id, $cross_type_id, $male_parent_type_id, $member_of_type_id, $family_name_type_id, $cross_experiment_cvterm, $female_parent);
286 elsif ($male_parent) {
287 $h->execute($female_parent_type_id, $cross_type_id, $male_parent_type_id, $member_of_type_id, $family_name_type_id, $cross_experiment_cvterm, $male_parent);
290 $h->execute($female_parent_type_id, $cross_type_id, $male_parent_type_id, $member_of_type_id, $family_name_type_id, $cross_experiment_cvterm);
293 my @cross_details = ();
294 while (my ($female_parent_id, $male_parent_id, $cross_entry_id, $female_parent_name, $male_parent_name, $cross_name, $cross_type, $family_id, $family_name, $project_id, $project_name) = $h->fetchrow_array()){
295 push @cross_details, [$female_parent_id, $female_parent_name, $male_parent_id, $male_parent_name, $cross_entry_id, $cross_name, $cross_type, $family_id, $family_name, $project_id, $project_name]
297 # print STDERR Dumper(\@cross_details);
298 return \
@cross_details;
302 =head2 get_cross_info_for_progeny
304 Usage: CXGN::Cross->get_cross_info_for_progeny( $schema, $female_parent_id, $male_parent_id, $progeny_id);
306 Ret: cross info for the cross that created the progeny
313 sub get_cross_info_for_progeny
{
316 my $female_parent_id = shift;
317 my $male_parent_id = shift;
318 my $progeny_id = shift;
320 my $male_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_parent", "stock_relationship")->cvterm_id();
321 my $female_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_parent", "stock_relationship")->cvterm_id();
322 my $cross_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross", "stock_type")->cvterm_id();
323 my $offspring_of_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'offspring_of', 'stock_relationship')->cvterm_id();
325 my $cross_experiment_type_cvterm_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross_experiment', 'experiment_type')->cvterm_id();
326 my $project_year_cvterm_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'project year', 'project_property')->cvterm_id();
328 my $q = "SELECT cross_entry.stock_id, cross_entry.uniquename, female_stock_relationship.value, year.value
329 FROM stock_relationship AS female_stock_relationship
330 JOIN stock AS cross_entry ON (cross_entry.stock_id=female_stock_relationship.object_id)
331 JOIN stock_relationship AS male_stock_relationship ON (cross_entry.stock_id=male_stock_relationship.object_id)
332 JOIN stock_relationship AS cross_to_progeny_rel ON (cross_entry.stock_id=cross_to_progeny_rel.object_id)
333 JOIN nd_experiment_stock ON (cross_entry.stock_id=nd_experiment_stock.stock_id)
334 JOIN nd_experiment USING(nd_experiment_id)
335 JOIN nd_experiment_project USING(nd_experiment_id)
336 JOIN project USING (project_id)
337 JOIN projectprop as year USING(project_id)
338 WHERE cross_entry.type_id= ? AND female_stock_relationship.type_id= ? AND female_stock_relationship.subject_id = ? AND male_stock_relationship.type_id= ? AND male_stock_relationship.subject_id = ? AND cross_to_progeny_rel.type_id = ? AND cross_to_progeny_rel.subject_id = ? AND nd_experiment.type_id = ? AND year.type_id = ?
339 ORDER BY female_stock_relationship.value, male_stock_relationship.subject_id";
341 my $h = $schema->storage->dbh()->prepare($q);
342 $h->execute($cross_typeid, $female_parent_typeid, $female_parent_id, $male_parent_typeid, $male_parent_id, $offspring_of_typeid, $progeny_id, $cross_experiment_type_cvterm_id, $project_year_cvterm_id);
346 while (my ($cross_entry_id, $cross_name, $cross_type, $year) = $h->fetchrow_array()){
347 push @cross_info, [$cross_entry_id, $cross_name, $cross_type, $year];
349 #print STDERR Dumper(\@cross_info);
350 if (scalar(@cross_info)>1){
351 print STDERR
"There is more than one (".scalar(@cross_info).") cross linked to this progeny\n";
353 if (scalar(@cross_info)>0){
354 return $cross_info[0];
361 =head2 get_progeny_info
363 Usage: CXGN::Cross->get_progeny_info($schema, $female_parent, $male_parent);
364 Desc: Class method. Used for the cross search for searching with either female or male parents.
372 sub get_progeny_info
{
376 my $female_parent = shift;
377 my $male_parent = shift;
379 my $male_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_parent", "stock_relationship")->cvterm_id();
380 my $female_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_parent", "stock_relationship")->cvterm_id();
381 my $accession_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "accession", "stock_type")->cvterm_id();
383 my $where_clause = "";
384 if ($female_parent && $male_parent) {
385 $where_clause = "WHERE female_parent.uniquename = ? AND male_parent.uniquename = ?";
387 elsif ($female_parent) {
388 $where_clause = "WHERE female_parent.uniquename = ? ORDER BY male_parent.uniquename";
390 elsif ($male_parent) {
391 $where_clause = "WHERE male_parent.uniquename = ? ORDER BY female_parent.uniquename";
394 my $q = "SELECT DISTINCT female_parent.stock_id, female_parent.uniquename, male_parent.stock_id, male_parent.uniquename, progeny.stock_id, progeny.uniquename, stock_relationship1.value
395 FROM stock_relationship as stock_relationship1
396 INNER JOIN stock AS female_parent ON (stock_relationship1.subject_id = female_parent.stock_id) AND stock_relationship1.type_id = ?
397 INNER JOIN stock AS progeny ON (stock_relationship1.object_id = progeny.stock_id) AND progeny.type_id = ?
398 LEFT JOIN stock_relationship AS stock_relationship2 ON (progeny.stock_id = stock_relationship2.object_id) AND stock_relationship2.type_id = ?
399 LEFT JOIN stock AS male_parent ON (stock_relationship2.subject_id = male_parent.stock_id)
402 my $h = $schema->storage->dbh()->prepare($q);
404 if($female_parent && $male_parent){
405 $h->execute($female_parent_typeid, $accession_typeid, $male_parent_typeid, $female_parent, $male_parent);
407 elsif ($female_parent) {
408 $h->execute($female_parent_typeid, $accession_typeid, $male_parent_typeid, $female_parent);
410 elsif ($male_parent) {
411 $h->execute($female_parent_typeid, $accession_typeid, $male_parent_typeid, $male_parent);
414 $h->execute($female_parent_typeid, $accession_typeid, $male_parent_typeid);
417 my @progeny_info = ();
418 while (my($female_parent_id, $female_parent_name, $male_parent_id, $male_parent_name, $progeny_id, $progeny_name, $cross_type) = $h->fetchrow_array()){
420 push @progeny_info, [$female_parent_id, $female_parent_name, $male_parent_id, $male_parent_name, $progeny_id, $progeny_name, $cross_type]
422 #print STDERR Dumper(\@progeny_info);
423 return \
@progeny_info;
427 =head2 get_crosses_in_crossing_experiment
430 Returns all cross names and ids in a specific crossing_experiment.
431 Example: my @crosses = CXGN::Cross->get_crosses_in_crossing_experiment($schema, $trial_id)
435 sub get_crosses_in_crossing_experiment
{
437 my $schema = $self->schema;
438 my $trial_id = $self->trial_id;
440 my $cross_stock_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross', 'stock_type')->cvterm_id;
442 my $q = "SELECT stock.stock_id, stock.uniquename FROM nd_experiment_project
443 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
444 JOIN stock on (nd_experiment_stock.stock_id = stock.stock_id)
445 WHERE stock.type_id = ? AND nd_experiment_project.project_id = ?";
447 my $h = $schema->storage->dbh()->prepare($q);
448 $h->execute($cross_stock_type_id, $trial_id);
451 while(my($cross_id, $cross_name) = $h->fetchrow_array()){
452 push @data, [$cross_id, $cross_name]
459 =head2 get_female_accessions_in_crossing_experiment
462 Returns all female accession names and ids in a specific crossing_experiment.
463 Example: my @female_accessions = CXGN::Cross->get_female_accessions_in_crossing_experiment($schema, $trial_id)
467 sub get_female_accessions_in_crossing_experiment
{
469 my $schema = $self->schema;
470 my $trial_id = $self->trial_id;
472 my $female_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_parent", "stock_relationship")->cvterm_id();
474 my $q = "SELECT DISTINCT stock.stock_id, stock.uniquename FROM nd_experiment_project
475 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
476 JOIN stock_relationship ON (nd_experiment_stock.stock_id = stock_relationship.object_id) AND stock_relationship.type_id = ?
477 JOIN stock on (stock_relationship.subject_id = stock.stock_id)
478 WHERE nd_experiment_project.project_id = ?";
480 my $h = $schema->storage->dbh()->prepare($q);
481 $h->execute($female_parent_typeid, $trial_id);
484 while(my($female_accession_id, $female_accession_name) = $h->fetchrow_array()){
485 push @data, [$female_accession_id, $female_accession_name]
492 =head2 get_male_accessions_in_crossing_experiment
495 Returns all male accession names and ids in a specific crossing_experiment.
496 Example: my @male_accessions = CXGN::Cross->get_male_accessions_in_crossing_experiment($schema, $trial_id)
500 sub get_male_accessions_in_crossing_experiment
{
502 my $schema = $self->schema;
503 my $trial_id = $self->trial_id;
505 my $male_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_parent", "stock_relationship")->cvterm_id();
507 my $q = "SELECT DISTINCT stock.stock_id, stock.uniquename FROM nd_experiment_project
508 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
509 JOIN stock_relationship ON (nd_experiment_stock.stock_id = stock_relationship.object_id) AND stock_relationship.type_id = ?
510 JOIN stock on (stock_relationship.subject_id = stock.stock_id)
511 WHERE nd_experiment_project.project_id = ?";
513 my $h = $schema->storage->dbh()->prepare($q);
514 $h->execute($male_parent_typeid, $trial_id);
517 while(my($male_accession_id, $male_accession_name) = $h->fetchrow_array()){
518 push @data, [$male_accession_id, $male_accession_name]
525 =head2 get_female_plots_in_crossing_experiment
528 Returns all female plot names and ids in a specific crossing_experiment.
529 Example: my @female_plots = CXGN::Cross->get_female_plots_in_crossing_experiment($schema, $trial_id)
533 sub get_female_plots_in_crossing_experiment
{
535 my $schema = $self->schema;
536 my $trial_id = $self->trial_id;
538 my $female_plot_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_plot_of", "stock_relationship")->cvterm_id();
540 my $q = "SELECT DISTINCT stock.stock_id, stock.uniquename FROM nd_experiment_project
541 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
542 JOIN stock_relationship ON (nd_experiment_stock.stock_id = stock_relationship.object_id) AND stock_relationship.type_id = ?
543 JOIN stock on (stock_relationship.subject_id = stock.stock_id)
544 WHERE nd_experiment_project.project_id = ?";
546 my $h = $schema->storage->dbh()->prepare($q);
547 $h->execute($female_plot_typeid, $trial_id);
550 while(my($female_plot_id, $female_plot_name) = $h->fetchrow_array()){
551 push @data, [$female_plot_id, $female_plot_name]
558 =head2 get_male_plots_in_crossing_experiment
561 Returns all male plot names and ids in a specific crossing_experiment.
562 Example: my @male_plots = CXGN::Cross->get_female_plots_in_crossing_experiment($schema, $trial_id)
566 sub get_male_plots_in_crossing_experiment
{
568 my $schema = $self->schema;
569 my $trial_id = $self->trial_id;
571 my $male_plot_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_plot_of", "stock_relationship")->cvterm_id();
573 my $q = "SELECT DISTINCT stock.stock_id, stock.uniquename FROM nd_experiment_project
574 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
575 JOIN stock_relationship ON (nd_experiment_stock.stock_id = stock_relationship.object_id) AND stock_relationship.type_id = ?
576 JOIN stock on (stock_relationship.subject_id = stock.stock_id)
577 WHERE nd_experiment_project.project_id = ?";
579 my $h = $schema->storage->dbh()->prepare($q);
580 $h->execute($male_plot_typeid, $trial_id);
583 while(my($male_plot_id, $male_plot_name) = $h->fetchrow_array()){
584 push @data, [$male_plot_id, $male_plot_name]
591 =head2 get_female_plants_in_crossing_experiment
594 Returns all female plant names and ids in a specific crossing_experiment.
595 Example: my @female_plants = CXGN::Cross->get_female_plants_in_crossing_experiment($schema, $trial_id)
599 sub get_female_plants_in_crossing_experiment
{
601 my $schema = $self->schema;
602 my $trial_id = $self->trial_id;
604 my $female_plant_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_plant_of", "stock_relationship")->cvterm_id();
606 my $q = "SELECT DISTINCT stock.stock_id, stock.uniquename FROM nd_experiment_project
607 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
608 JOIN stock_relationship ON (nd_experiment_stock.stock_id = stock_relationship.object_id) AND stock_relationship.type_id = ?
609 JOIN stock on (stock_relationship.subject_id = stock.stock_id)
610 WHERE nd_experiment_project.project_id = ?";
612 my $h = $schema->storage->dbh()->prepare($q);
613 $h->execute($female_plant_typeid, $trial_id);
616 while(my($female_plant_id, $female_plant_name) = $h->fetchrow_array()){
617 push @data, [$female_plant_id, $female_plant_name]
624 =head2 get_male_plants_in_crossing_experiment
627 Returns all male plant names and ids in a specific crossing_experiment.
628 Example: my @male_plants = CXGN::Cross->get_male_plants_in_crossing_experiment($schema, $trial_id)
632 sub get_male_plants_in_crossing_experiment
{
634 my $schema = $self->schema;
635 my $trial_id = $self->trial_id;
637 my $male_plant_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_plant_of", "stock_relationship")->cvterm_id();
639 my $q = "SELECT DISTINCT stock.stock_id, stock.uniquename FROM nd_experiment_project
640 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
641 JOIN stock_relationship ON (nd_experiment_stock.stock_id = stock_relationship.object_id) AND stock_relationship.type_id = ?
642 JOIN stock on (stock_relationship.subject_id = stock.stock_id)
643 WHERE nd_experiment_project.project_id = ?";
645 my $h = $schema->storage->dbh()->prepare($q);
646 $h->execute($male_plant_typeid, $trial_id);
649 while(my($male_plant_id, $male_plant_name) = $h->fetchrow_array()){
650 push @data, [$male_plant_id, $male_plant_name]
657 =head2 get_crosses_and_details_in_crossingtrial
660 Returns all cross names, ids, cross_combinations, cross types and parent info in a specific crossing_experiment.
661 Example: my @crosses_details = CXGN::Cross->get_crosses_and_details_in_crossingtrial($schema, $trial_id)
665 sub get_crosses_and_details_in_crossingtrial
{
667 my $schema = $self->schema;
668 my $trial_id = $self->trial_id;
670 my $cross_combination_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_combination", "stock_property")->cvterm_id();
671 my $male_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_parent", "stock_relationship")->cvterm_id();
672 my $female_parent_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_parent", "stock_relationship")->cvterm_id();
673 my $female_plot_of_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_plot_of", "stock_relationship")->cvterm_id();
674 my $male_plot_of_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_plot_of", "stock_relationship")->cvterm_id();
675 my $female_plant_of_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "female_plant_of", "stock_relationship")->cvterm_id();
676 my $male_plant_of_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "male_plant_of", "stock_relationship")->cvterm_id();
677 my $ploidy_level_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "ploidy_level", "stock_property")->cvterm_id();
679 my $q = "SELECT stock1.stock_id AS cross_id, stock1.uniquename AS cross_name, stockprop.value AS cross_combination, stock_relationship1.value AS cross_type, stock2.stock_id AS female_id,
680 stock2.uniquename AS female_name, stockprop2.value AS female_ploidy, stock3.stock_id AS male_id, stock3.uniquename AS male_name, stockprop3.value AS male_ploidy, stock4.stock_id AS female_plot_id, stock4.uniquename AS female_plot_name,
681 stock5.stock_id AS male_plot_id, stock5.uniquename AS male_plot_name, stock6.stock_id AS female_plant_id, stock6.uniquename AS female_plant_name, stock7.stock_id AS male_plant_id, stock7.uniquename AS male_plant_name
682 FROM nd_experiment_project JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
683 JOIN stock AS stock1 ON (nd_experiment_stock.stock_id = stock1.stock_id)
684 LEFT JOIN stockprop ON (stock1.stock_id = stockprop.stock_id) AND stockprop.type_id =?
685 JOIN stock_relationship AS stock_relationship1 ON (stock1.stock_id = stock_relationship1.object_id) AND stock_relationship1.type_id = ?
686 JOIN stock AS stock2 ON (stock_relationship1.subject_id = stock2.stock_id)
687 LEFT JOIN stockprop AS stockprop2 ON (stock2.stock_id = stockprop2.stock_id) AND stockprop2.type_id = ?
688 LEFT JOIN stock_relationship AS stock_relationship2 ON (stock1.stock_id = stock_relationship2.object_id) AND stock_relationship2.type_id = ?
689 LEFT JOIN stock AS stock3 ON (stock_relationship2.subject_id = stock3.stock_id)
690 LEFT JOIN stockprop AS stockprop3 ON (stock3.stock_id = stockprop3.stock_id) AND stockprop3.type_id = ?
691 LEFT JOIN stock_relationship AS stock_relationship3 ON (stock1.stock_id = stock_relationship3.object_id) AND stock_relationship3.type_id = ?
692 LEFT JOIN stock AS stock4 ON (stock_relationship3.subject_id = stock4.stock_id)
693 LEFT JOIN stock_relationship AS stock_relationship4 ON (stock1.stock_id = stock_relationship4.object_id) AND stock_relationship4.type_id = ?
694 LEFT JOIN stock AS stock5 ON (stock_relationship4.subject_id = stock5.stock_id)
695 LEFT JOIN stock_relationship AS stock_relationship5 ON (stock1.stock_id = stock_relationship5.object_id) AND stock_relationship5.type_id = ?
696 LEFT JOIN stock AS stock6 ON (stock_relationship5.subject_id = stock6.stock_id)
697 LEFT JOIN stock_relationship AS stock_relationship6 ON (stock1.stock_id = stock_relationship6.object_id) AND stock_relationship6.type_id = ?
698 LEFT JOIN stock AS stock7 ON (stock_relationship6.subject_id = stock7.stock_id)
699 WHERE nd_experiment_project.project_id = ? ORDER BY cross_id ASC";
701 my $h = $schema->storage->dbh()->prepare($q);
703 $h->execute($cross_combination_type_id, $female_parent_typeid, $ploidy_level_type_id, $male_parent_typeid, $ploidy_level_type_id, $female_plot_of_typeid, $male_plot_of_typeid, $female_plant_of_typeid, $male_plant_of_typeid, $trial_id);
706 while(my($cross_id, $cross_name, $cross_combination, $cross_type, $female_parent_id, $female_parent_name, $female_ploidy, $male_parent_id, $male_parent_name, $male_ploidy, $female_plot_id, $female_plot_name, $male_plot_id, $male_plot_name, $female_plant_id, $female_plant_name, $male_plant_id, $male_plant_name) = $h->fetchrow_array()){
707 push @data, [$cross_id, $cross_name, $cross_combination, $cross_type, $female_parent_id, $female_parent_name, $female_ploidy, $male_parent_id, $male_parent_name, $male_ploidy, $female_plot_id, $female_plot_name, $male_plot_id, $male_plot_name, $female_plant_id, $female_plant_name, $male_plant_id, $male_plant_name]
712 =head2 get_cross_properties_trial
715 Returns all cross_info in a specific trial.
716 Example: my @cross_info = CXGN::Cross->get_cross_properties_trial($schema, $trial_id);
720 sub get_cross_properties_trial
{
722 my $schema = $self->schema;
723 my $trial_id = $self->trial_id;
725 my $cross_combination_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_combination", "stock_property")->cvterm_id();
726 my $cross_props_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "crossing_metadata_json", "stock_property")->cvterm_id();
728 my $q = "SELECT stock.stock_id, stock.uniquename, stockprop1.value, stockprop2.value FROM nd_experiment_project
729 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
730 JOIN stock ON (nd_experiment_stock.stock_id = stock.stock_id)
731 LEFT JOIN stockprop AS stockprop1 ON (stock.stock_id = stockprop1.stock_id) AND stockprop1.type_id = ?
732 LEFT JOIN stockprop AS stockprop2 ON (stock.stock_id = stockprop2.stock_id) AND stockprop2.type_id = ?
733 WHERE nd_experiment_project.project_id = ? ORDER BY stock.stock_id ASC";
735 my $h = $schema->storage->dbh()->prepare($q);
737 $h->execute($cross_combination_typeid, $cross_props_typeid, $trial_id);
741 while(my($cross_id, $cross_name, $cross_combination, $cross_props) = $h->fetchrow_array()){
742 #print STDERR Dumper $cross_props;
744 my $cross_props_hash = decode_json
$cross_props;
745 push @data, [$cross_id, $cross_name, $cross_combination, $cross_props_hash]
747 push @data, [$cross_id, $cross_name, $cross_combination, $cross_props]
756 =head2 get_seedlots_from_crossingtrial
759 Returns all seedlots derived from crosses in a specific crossing_experiment.
760 Example: my @crosses_seedlots = CXGN::Cross->get_seedlots_from_crossingtrial($schema, $trial_id)
764 sub get_seedlots_from_crossingtrial
{
766 my $schema = $self->schema;
767 my $trial_id = $self->trial_id;
769 my $collection_of_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "collection_of", "stock_relationship")->cvterm_id();
771 my $q = "SELECT stock1.stock_id, stock1.uniquename, stock2.stock_id, stock2.uniquename FROM nd_experiment_project
772 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
773 JOIN stock as stock1 on (nd_experiment_stock.stock_id = stock1.stock_id)
774 LEFT JOIN stock_relationship ON (stock1.stock_id = stock_relationship.subject_id) and stock_relationship.type_id = ?
775 LEFT JOIN stock as stock2 ON (stock_relationship.object_id = stock2.stock_id)
776 WHERE nd_experiment_project.project_id = ? ORDER BY stock1.stock_id ASC";
778 my $h = $schema->storage->dbh()->prepare($q);
780 $h->execute($collection_of_type_id, $trial_id);
783 while(my($cross_id, $cross_name, $seedlot_id, $seedlot_uniquename) = $h->fetchrow_array()){
784 push @data, [$cross_id, $cross_name, $seedlot_id, $seedlot_uniquename]
791 =head2 get_cross_progenies_trial
794 Get numbers of progenies and family names of all the crosses in a crossing_experiment.
795 Example: my @progenies_info = CXGN::Cross->get_cross_progenies_trial($schema, $trial_id)
799 sub get_cross_progenies_trial
{
801 my $schema = $self->schema;
802 my $trial_id = $self->trial_id;
804 my $cross_combination_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_combination", "stock_property")->cvterm_id();
805 my $offspring_of_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "offspring_of", "stock_relationship")->cvterm_id();
806 my $family_name_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "family_name", "stock_type")->cvterm_id();
807 my $cross_member_of_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_member_of", "stock_relationship")->cvterm_id();
809 my $q = "SELECT cross_table.cross_id, cross_table.cross_name, cross_table.cross_combination, cross_table.family_id, cross_table.family_name, progeny_count_table.progeny_number
811 (SELECT stock.stock_id AS cross_id, stock.uniquename AS cross_name, stockprop.value AS cross_combination, stock2.stock_id AS family_id, stock2.uniquename AS family_name
812 FROM nd_experiment_project JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
813 JOIN stock ON (nd_experiment_stock.stock_id = stock.stock_id)
814 LEFT JOIN stockprop ON (stock.stock_id = stockprop.stock_id) AND stockprop.type_id = ?
815 LEFT JOIN stock_relationship ON (stock.stock_id = stock_relationship.subject_id) AND stock_relationship.type_id = ?
816 LEFT JOIN stock AS stock2 ON (stock_relationship.object_id = stock2.stock_id) AND stock2.type_id = ?
817 WHERE nd_experiment_project.project_id = ?) AS cross_table
819 (SELECT DISTINCT stock.stock_id AS cross_id, COUNT (stock_relationship.subject_id) AS progeny_number
820 FROM nd_experiment_project JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
821 JOIN stock ON (nd_experiment_stock.stock_id = stock.stock_id)
822 LEFT JOIN stock_relationship ON (stock.stock_id = stock_relationship.object_id) AND stock_relationship.type_id = ?
823 WHERE nd_experiment_project.project_id = ? GROUP BY cross_id) AS progeny_count_table
824 ON (cross_table.cross_id = progeny_count_table.cross_id) ORDER BY cross_id ASC";
826 my $h = $schema->storage->dbh()->prepare($q);
828 $h->execute($cross_combination_type_id, $cross_member_of_type_id, $family_name_type_id, $trial_id, $offspring_of_type_id, $trial_id);
831 while(my($cross_id, $cross_name, $cross_combination, $family_id, $family_name, $progeny_number) = $h->fetchrow_array()){
832 push @data, [$cross_id, $cross_name, $cross_combination, $family_id, $family_name, $progeny_number]
843 Usage: $cross->delete();
844 Desc: Deletes a cross
845 Ret: error string if error, undef otherwise
847 Side Effects: deletes project entry, nd_experiment entry, and stock entry.
857 print STDERR
"Delete cross ".$self->cross_name()."\n";
858 my $dbh = $self->schema()->storage()->dbh();
859 my $schema = $self->schema();
860 my $cross_id = $self->cross_stock_id();
865 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross", "stock_type")->cvterm_id();
866 my $cross_experiment_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_experiment", "experiment_type")->cvterm_id();
867 my $collection_of_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "collection_of", "stock_relationship")->cvterm_id();
868 my $field_layout_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'field_layout', 'experiment_type')->cvterm_id();
869 my $plot_of_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'plot_of', 'stock_relationship')->cvterm_id();
871 # checking if cross has associated seedlot
872 my $q = "SELECT stock_relationship.type_id, stock.uniquename FROM stock_relationship JOIN stock ON (stock_relationship.object_id = stock.stock_id) WHERE stock_relationship.subject_id = ?";
874 my $h = $self->schema->storage->dbh()->prepare($q);
876 $h->execute($cross_id);
878 while (my($type_id, $seedlot_name) = $h->fetchrow_array()) {
879 if ($type_id == $collection_of_type_id) {
880 print STDERR
"Cross has associated seedlot. Cannot delete.\n";
881 die "Cross has associated seedlot: $seedlot_name. Cannot delete.\n";
885 #checking if cross has associated trial
886 my $q2 = "SELECT nd_experiment_stock.type_id, project.name FROM stock_relationship JOIN nd_experiment_stock ON (stock_relationship.subject_id = nd_experiment_stock.stock_id) AND stock_relationship.type_id = ?
887 JOIN nd_experiment_project ON (nd_experiment_stock.nd_experiment_id = nd_experiment_project.nd_experiment_id)
888 JOIN project ON (nd_experiment_project.project_id = project.project_id) WHERE stock_relationship.object_id = ? ";
890 my $h2 = $self->schema->storage->dbh()->prepare($q2);
892 $h2->execute($plot_of_type_id, $cross_id);
894 while (my($type_id, $project_name) = $h2->fetchrow_array()) {
895 if ($type_id == $field_layout_type_id) {
896 print STDERR
"Cross has associated trial. Cannot delete.\n";
897 die "Cross has associated trial: $project_name. Cannot delete.\n";
901 #checking if any progeny has associated data
902 my $properties = $self->progeny_properties();
905 ($properties->{trials
} == 0) &&
906 ($properties->{traits
} == 0) &&
907 ($properties->{genotypes
} == 0) &&
908 ($properties->{images
} == 0);
911 print STDERR
"Cross has associated data. Cannot delete.\n";
912 die "Cross has associated data. ($properties->{trials} trials, $properties->{traits} traits and $properties->{genoytpes} genotypes. Cannot delete...\n";
915 print STDERR
"This cross has no associated data that would prevent deletion.";
918 #checking if the stock id has cross stock type
919 my $cross_rs = $schema->resultset("Stock::Stock")->find ({stock_id
=> $cross_id, type_id
=> $cross_type_id});
921 print STDERR
"This stock id is not a cross. Cannot delete.\n";
922 die "This stock id is not a cross. Cannot delete.\n";
925 #get nd_experiment_id with cross_experiment_type
927 my $nd_q = "SELECT nd_experiment.nd_experiment_id FROM nd_experiment_stock
928 JOIN nd_experiment ON (nd_experiment_stock.nd_experiment_id = nd_experiment.nd_experiment_id)
929 WHERE nd_experiment.type_id = ? AND nd_experiment_stock.stock_id = ?";
931 my $nd_h = $schema->storage->dbh()->prepare($nd_q);
932 $nd_h->execute($cross_experiment_type_id, $cross_id);
933 my @nd_experiment_ids= $nd_h->fetchrow_array();
934 if (scalar @nd_experiment_ids == 1) {
935 $experiment_id = $nd_experiment_ids[0];
937 print STDERR
"Error retrieving experiment id"."\n";
938 die "Error retrieving experiment id";
940 # print STDERR "ND EXPERIMENT ID =".Dumper($experiment_id)."\n";
942 #delete the nd_experiment_md_files entries
943 my $md_files_q = "DELETE FROM phenome.nd_experiment_md_files WHERE nd_experiment_id = ?";
944 my $md_files_h = $schema->storage->dbh()->prepare($md_files_q);
945 $md_files_h->execute($experiment_id);
947 # delete the nd_experiment entries
948 print STDERR
"Deleting nd_experiment entry for cross...\n";
949 my $q2= "delete from nd_experiment where nd_experiment.nd_experiment_id = ? AND nd_experiment.type_id = ?";
950 my $h2 = $dbh->prepare($q2);
951 $h2->execute($experiment_id, $cross_experiment_type_id);
953 # delete stock owner entries
955 print STDERR
"Deleting associated stock_owners...\n";
956 my $q3 = "delete from phenome.stock_owner where stock_id=?";
957 my $h3 = $dbh->prepare($q3);
958 $h3->execute($self->cross_stock_id());
960 # delete the stock entries
962 print STDERR
"Deleting the stock entry...\n";
963 my $q4 = "delete from stock where stock.stock_id=? and stock.type_id = ?";
964 my $h4 = $dbh->prepare($q4);
965 $h4->execute($self->cross_stock_id(), $cross_type_id);
967 print STDERR Dumper
($properties);
968 # delete the progeny...
970 print STDERR
"Deleting the progeny...\n";
971 my $q5 = "delete from stock where stock_id =?";
972 my $h5 = $dbh->prepare($q5);
973 foreach my $progeny (@
{$properties->{subjects
}}) {
975 if ($progeny->[2] eq "offspring_of") {
976 my $s = CXGN
::Stock
->new( { schema
=> $schema, stock_id
=> $progeny->[0]});
984 print STDERR
"An error occurred while deleting cross id ".$self->cross_stock_id()."$@\n";
994 sub progeny_properties
{
997 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($self->schema(), "cross", "stock_type")->cvterm_id();
999 print STDERR
"sub cross_deletion_possible...\n";
1000 my $q = "SELECT subject.stock_id, subject.uniquename, cvterm.name from stock join stock_relationship on (stock.stock_id=stock_relationship.object_id) join stock as subject on(stock_relationship.subject_id=subject.stock_id) join cvterm on (stock_relationship.type_id=cvterm.cvterm_id) where stock.stock_id = ? and stock.type_id=?";
1002 my $h = $self->schema->storage->dbh()->prepare($q);
1004 $h->execute($self->cross_stock_id(), $cross_type_id);
1009 my $has_genotypes = 0;
1012 while (my($stock_id, $name, $type) = $h->fetchrow_array()) {
1013 print STDERR
"ID $stock_id NAME $name TYPE $type\n";
1014 push @subjects, [$stock_id, $name, $type];
1016 if ($type eq "offspring_of") { # child
1017 my $s = CXGN
::Stock
->new( { schema
=> $self->schema(), stock_id
=> $stock_id });
1018 if (my @traits = $s->get_trait_list()) {
1019 print STDERR
"Associated traits: ".Dumper
(\
@traits);
1020 $has_traits += scalar(@traits);
1022 if (my @trials = $s->get_trials()) {
1023 print STDERR
"Associated trials: ".Dumper
(\
@trials);
1024 $has_trials += scalar(@trials);
1026 if (my $genotypeprop_ids = $s->get_genotypeprop_ids()) {
1027 print STDERR
"Associated genotypes: ".Dumper
($genotypeprop_ids);
1028 $has_genotypes += scalar(@
$genotypeprop_ids);
1030 if (my @image_ids = $s->get_image_ids()) {
1031 print STDERR
"Associated images: ".Dumper
(\
@image_ids);
1032 $has_images += scalar(@image_ids);
1037 traits
=> $has_traits,
1038 trials
=> $has_trials,
1039 genotypes
=> $has_genotypes,
1040 images
=> $has_images,
1041 subjects
=> \
@subjects,
1044 print STDERR Dumper
($data);
1049 sub get_cross_tissue_culture_samples
{
1051 my $cross_samples_cvterm = SGN
::Model
::Cvterm
->get_cvterm_row($self->schema, 'tissue_culture_data_json', 'stock_property')->cvterm_id();
1052 my $cross_samples = $self->schema->resultset("Stock::Stockprop")->find({stock_id
=> $self->cross_stock_id, type_id
=> $cross_samples_cvterm});
1054 my $samples_json_string;
1056 $samples_json_string = $cross_samples->value();
1059 my $samples_hash_ref ={};
1060 if($samples_json_string){
1061 $samples_hash_ref = decode_json
$samples_json_string;
1064 return $samples_hash_ref;
1068 =head2 get_pedigree_male_parents
1071 Returns all male parents that were crossed with a spefified female parent.
1072 Example: my @male_parents = CXGN::Cross->get_pedigree_male_parents($schema, $pedigree_female_parent)
1076 sub get_pedigree_male_parents
{
1079 my $pedigree_female_parent = shift;
1081 my $male_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_parent', 'stock_relationship')->cvterm_id();
1082 my $female_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_parent', 'stock_relationship')->cvterm_id();
1083 my $accession_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'accession', 'stock_type')->cvterm_id();
1085 my $dbh = $schema->storage->dbh();
1087 my $q = "SELECT DISTINCT male_parent.stock_id, male_parent.uniquename FROM stock as female_parent
1088 INNER JOIN stock_relationship AS stock_relationship1 ON (female_parent.stock_id=stock_relationship1.subject_id) AND stock_relationship1.type_id= ?
1089 INNER JOIN stock AS check_type ON (stock_relationship1.object_id=check_type.stock_id) AND check_type.type_id = ?
1090 INNER JOIN stock_relationship AS stock_relationship2 ON (stock_relationship1.object_id=stock_relationship2.object_id) AND stock_relationship2.type_id = ?
1091 INNER JOIN stock AS male_parent ON (male_parent.stock_id=stock_relationship2.subject_id)
1092 WHERE female_parent.uniquename= ? ORDER BY male_parent.uniquename ASC";
1094 my $h = $dbh->prepare($q);
1095 $h->execute($female_parent_type_id, $accession_type_id, $male_parent_type_id, $pedigree_female_parent);
1097 my @male_parents=();
1098 while(my ($male_parent_id, $male_parent_name) = $h->fetchrow_array()){
1099 push @male_parents, [$male_parent_name];
1102 return \
@male_parents;
1107 =head2 get_pedigree_female_parents
1110 Returns all female parents that were crossed with a spefified male parent.
1111 Example: my @female_parents = CXGN::Cross->get_pedigree_female_parents($schema, $pedigree_male_parent)
1115 sub get_pedigree_female_parents
{
1118 my $pedigree_male_parent = shift;
1120 my $male_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_parent', 'stock_relationship')->cvterm_id();
1121 my $female_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_parent', 'stock_relationship')->cvterm_id();
1122 my $accession_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'accession', 'stock_type')->cvterm_id();
1124 my $dbh = $schema->storage->dbh();
1126 my $q = "SELECT DISTINCT female_parent.stock_id, female_parent.uniquename FROM stock as male_parent
1127 INNER JOIN stock_relationship AS stock_relationship1 ON (male_parent.stock_id=stock_relationship1.subject_id) AND stock_relationship1.type_id= ?
1128 INNER JOIN stock AS check_type ON (stock_relationship1.object_id=check_type.stock_id) AND check_type.type_id = ?
1129 INNER JOIN stock_relationship AS stock_relationship2 ON (stock_relationship1.object_id=stock_relationship2.object_id) AND stock_relationship2.type_id = ?
1130 INNER JOIN stock AS female_parent ON (female_parent.stock_id=stock_relationship2.subject_id)
1131 WHERE male_parent.uniquename= ? ORDER BY female_parent.uniquename ASC";
1134 my $h = $dbh->prepare($q);
1135 $h->execute($male_parent_type_id, $accession_type_id, $female_parent_type_id, $pedigree_male_parent);
1137 my @female_parents=();
1138 while(my ($female_parent_id, $female_parent_name) = $h->fetchrow_array()){
1139 push @female_parents, [$female_parent_name];
1142 return \
@female_parents;
1147 =head2 get_cross_male_parents
1150 Returns all male parents that were crossed with a spefified male parent.
1151 Example: my @male_parents = CXGN::Cross->get_cross_male_parents($schema, $cross_female_parent)
1155 sub get_cross_male_parents
{
1158 my $cross_female_parent = shift;
1160 my $male_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_parent', 'stock_relationship')->cvterm_id();
1161 my $female_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_parent', 'stock_relationship')->cvterm_id();
1162 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross', 'stock_type')->cvterm_id();
1164 my $dbh = $schema->storage->dbh();
1166 my $q = "SELECT DISTINCT male_parent.stock_id, male_parent.uniquename FROM stock as female_parent
1167 INNER JOIN stock_relationship AS stock_relationship1 ON (female_parent.stock_id=stock_relationship1.subject_id)
1168 INNER JOIN stock AS check_type ON (stock_relationship1.object_id=check_type.stock_id)
1169 LEFT JOIN stock_relationship AS stock_relationship2 ON (stock_relationship1.object_id = stock_relationship2.object_id)
1170 LEFT JOIN stock AS male_parent ON (male_parent.stock_id=stock_relationship2.subject_id)
1171 WHERE female_parent.uniquename = ? AND stock_relationship1.type_id = ? AND check_type.type_id = ? AND stock_relationship2.type_id = ?
1172 ORDER BY male_parent.uniquename ASC";
1174 my $h = $dbh->prepare($q);
1175 $h->execute($cross_female_parent, $female_parent_type_id, $cross_type_id, $male_parent_type_id );
1177 my @male_parents=();
1178 while(my ($male_parent_id, $male_parent_name) = $h->fetchrow_array()){
1179 push @male_parents, [$male_parent_name];
1182 return \
@male_parents;
1187 =head2 get_cross_female_parents
1190 Returns all female parents that were crossed with a spefified male parent.
1191 Example: my @male_parents = CXGN::Cross->get_cross_male_parents($schema, $cross_female_parent)
1195 sub get_cross_female_parents
{
1198 my $cross_male_parent = shift;
1200 my $male_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'male_parent', 'stock_relationship')->cvterm_id();
1201 my $female_parent_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'female_parent', 'stock_relationship')->cvterm_id();
1202 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross', 'stock_type')->cvterm_id();
1204 my $dbh = $schema->storage->dbh();
1206 my $q = "SELECT DISTINCT female_parent.stock_id, female_parent.uniquename FROM stock as male_parent
1207 INNER JOIN stock_relationship AS stock_relationship1 ON (male_parent.stock_id=stock_relationship1.subject_id)
1208 INNER JOIN stock AS check_type ON (stock_relationship1.object_id=check_type.stock_id)
1209 LEFT JOIN stock_relationship AS stock_relationship2 ON (stock_relationship1.object_id = stock_relationship2.object_id)
1210 LEFT JOIN stock AS female_parent ON (female_parent.stock_id=stock_relationship2.subject_id)
1211 WHERE male_parent.uniquename = ? AND stock_relationship1.type_id = ? AND check_type.type_id = ? AND stock_relationship2.type_id = ?
1212 ORDER BY female_parent.uniquename ASC";
1214 my $h = $dbh->prepare($q);
1215 $h->execute($cross_male_parent, $male_parent_type_id, $cross_type_id, $female_parent_type_id );
1217 my @female_parents=();
1218 while(my ($female_parent_id, $female_parent_name) = $h->fetchrow_array()){
1219 push @female_parents, [$female_parent_name];
1222 return \
@female_parents;
1227 =head2 get_cross_identifiers_in_crossing_experiment
1230 Returns all cross identifiers in a specific crossing_experiment together with the corresponding cross unique ids.
1231 Example: my $crosses = CXGN::Cross->new({schema => $schema, trial_id => $crossing_experiment_id});
1232 my $identifiers = $crosses->get_cross_identifiers_in_crossing_experiment();
1236 sub get_cross_identifiers_in_crossing_experiment
{
1238 my $schema = $self->schema;
1239 my $trial_id = $self->trial_id;
1241 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross', 'stock_type')->cvterm_id;
1242 my $cross_identifier_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross_identifier', 'stock_property')->cvterm_id;
1244 my $q = "SELECT stock.uniquename, stockprop.value FROM nd_experiment_project
1245 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
1246 JOIN stock ON (nd_experiment_stock.stock_id = stock.stock_id) AND stock.type_id = ?
1247 JOIN stockprop ON (stock.stock_id = stockprop.stock_id)
1248 WHERE stockprop.type_id = ? AND nd_experiment_project.project_id = ?";
1250 my $h = $schema->storage->dbh()->prepare($q);
1251 $h->execute($cross_type_id, $cross_identifier_type_id, $trial_id);
1253 my %cross_identifier_hash;
1254 while(my($cross_unique_id, $cross_identifier) = $h->fetchrow_array()){
1255 $cross_identifier_hash{$cross_identifier} = $cross_unique_id;
1258 return \
%cross_identifier_hash;
1262 =head2 get_cross_additional_info_trial
1265 Returns all cross_additional_info in a specific trial.
1266 Example: my @cross_additional_info = CXGN::Cross->get_cross_additional_trial($schema, $trial_id);
1270 sub get_cross_additional_info_trial
{
1272 my $schema = $self->schema;
1273 my $trial_id = $self->trial_id;
1275 my $cross_combination_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_combination", "stock_property")->cvterm_id();
1276 my $cross_additional_info_typeid = SGN
::Model
::Cvterm
->get_cvterm_row($schema, "cross_additional_info", "stock_property")->cvterm_id();
1278 my $q = "SELECT stock.stock_id, stock.uniquename, stockprop1.value, stockprop2.value FROM nd_experiment_project
1279 JOIN nd_experiment_stock ON (nd_experiment_project.nd_experiment_id = nd_experiment_stock.nd_experiment_id)
1280 JOIN stock ON (nd_experiment_stock.stock_id = stock.stock_id)
1281 LEFT JOIN stockprop AS stockprop1 ON (stock.stock_id = stockprop1.stock_id) AND stockprop1.type_id = ?
1282 LEFT JOIN stockprop AS stockprop2 ON (stock.stock_id = stockprop2.stock_id) AND stockprop2.type_id = ?
1283 WHERE nd_experiment_project.project_id = ?";
1285 my $h = $schema->storage->dbh()->prepare($q);
1287 $h->execute($cross_combination_typeid, $cross_additional_info_typeid, $trial_id);
1291 while(my($cross_id, $cross_name, $cross_combination, $cross_additional_info_json) = $h->fetchrow_array()){
1292 #print STDERR Dumper $cross_props;
1293 if ($cross_additional_info_json){
1294 my $cross_additional_info_hash = decode_json
$cross_additional_info_json;
1295 push @data, [$cross_id, $cross_name, $cross_combination, $cross_additional_info_hash]
1297 push @data, [$cross_id, $cross_name, $cross_combination, $cross_additional_info_json]
1305 =head2 get_nd_experiment_id_with_type_cross_experiment
1310 sub get_nd_experiment_id_with_type_cross_experiment
{
1312 my $schema = $self->schema;
1313 my $cross_name = $self->cross_name();
1316 # print STDERR "CROSS NAME =".Dumper($cross_name)."\n";
1317 my $cross_experiment_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross_experiment', 'experiment_type')->cvterm_id;
1318 my $cross_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'cross', 'stock_type')->cvterm_id();
1320 my $cross_rs = $schema->resultset("Stock::Stock")->find( { uniquename
=> $cross_name, type_id
=> $cross_type_id});
1323 $cross_id = $cross_rs->stock_id();
1325 print STDERR
"Error retrieving cross ID"."\n";
1329 my $q = "SELECT nd_experiment.nd_experiment_id FROM nd_experiment_stock
1330 JOIN nd_experiment ON (nd_experiment_stock.nd_experiment_id = nd_experiment.nd_experiment_id)
1331 WHERE nd_experiment.type_id = ? AND nd_experiment_stock.stock_id = ?";
1333 my $h = $schema->storage->dbh()->prepare($q);
1334 $h->execute($cross_experiment_type_id, $cross_id);
1335 my @nd_experiment_ids= $h->fetchrow_array();
1336 if (scalar @nd_experiment_ids == 1) {
1337 $experiment_id = $nd_experiment_ids[0];
1339 print STDERR
"Error retrieving experiment ID"."\n";
1343 return $experiment_id;
1347 =head2 get_intercross_file_metadata
1352 sub get_intercross_file_metadata
{
1354 my $schema = $self->schema;
1355 my $crossing_experiment_id = $self->trial_id();
1356 my $project_type_cvterm_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'crossing_trial', 'project_type')->cvterm_id();
1357 my $file_metadata_json_type_id = SGN
::Model
::Cvterm
->get_cvterm_row($schema, 'file_metadata_json', 'project_property')->cvterm_id;
1359 my $projectprop_rs = $schema->resultset("Project::Projectprop")->find({ project_id
=> $crossing_experiment_id, type_id
=> $file_metadata_json_type_id });
1363 my $dbh = $schema->storage->dbh();
1364 if ($projectprop_rs){
1365 my $file_metadata_json = $projectprop_rs->value();
1366 my $file_metadata = decode_json
$file_metadata_json;
1367 my $intercross_download_files = $file_metadata->{'intercross_download'};
1368 my %intercross_download_hash = %{$intercross_download_files};
1369 @file_ids = keys %intercross_download_hash;
1370 if (scalar @file_ids > 0) {
1371 foreach my $id (@file_ids){
1373 my $q = "SELECT f.file_id, m.create_date, p.sp_person_id, p.username, f.basename, f.dirname, f.filetype
1374 FROM metadata.md_files AS f
1375 JOIN metadata.md_metadata as m ON (f.metadata_id = m.metadata_id)
1376 JOIN sgn_people.sp_person as p ON (p.sp_person_id = m.create_person_id) WHERE f.file_id = ?
1377 ORDER BY f.file_id ASC";
1379 my $h = $dbh->prepare($q);
1381 @each_row = $h->fetchrow_array();
1382 push @file_info, [@each_row];