cgi-bin/community/feature/200405.pl

   1 use strict;
   2 use CXGN::Page;
   3 my $page=CXGN::Page->new('200405.html','html2pl converter');
   4 $page->header('The Choi Lab');
   5 print<<END_HEREDOC;
   6
   7   <center>
   8     <h1>Plant Genomics Lab. at KRIBB</h1><img src="/static_content/community/feature/200405-1.jpg"
   9     width="720" height="540" border="0" alt=
  10     "Members of the Choi Lab" />
  11   </center>
  12
  13   <h2>Research Highlight</h2>
  14
  15   <p>Our main interest is the application of functional genomics
  16   tools to understand plant defense mechanism at the molecular
  17   level. For this object, we have been using the non-host resistant
  18   interaction between hot pepper (Capsicum annuum L.) and soybean
  19   pustule pathogen Xag (Xanthomonas axonopodis pv. glycines).</p>
  20
  21   <p>First approach was the collection of massive hot pepper ESTs
  22   from divergent cDNA libraries (detail information is shown in
  23   <a href=
  24   "http://plant.pdrc.re.kr/ks200201/pepper.html">http://plant.pdrc.re.kr/ks200201/pepper.html</a>
  25   and <a href=
  26   "/content/sgn_data.pl">http://www.sgn.cornell.edu/content/sgn_data.pl</a>).
  27   We have got more than 10,000 unique hot pepper genes (<a href=
  28   "http://ted.bti.cornell.edu/pepper/">http://ted.bti.cornell.edu/pepper/</a>).</p>
  29
  30   <p>Next, we made the 5K cDNA microarray using unique hot pepper
  31   ESTs to monitor the global gene expression response to the
  32   pathogen inoculation, and finally we could get the candidate
  33   genes involved plant defense reaction. The pepper gene expression
  34   profiles were constructed as database and released to public
  35   (<a href=
  36   "http://plant.pdrc.re.kr:7777/index.html">http://plant.pdrc.re.kr:7777/index.html</a>).
  37   Currently we are building 10K cDNA microarray and getting more
  38   expression data from divergent stress situation, developmental
  39   stage and specific organs.</p>
  40
  41   <p>Recently, we established VIGS (Virus Induced Gene Silencing)
  42   method in hot pepper plant for the functional characterization of
  43   defense related genes. The identification of functional roles of
  44   defense related genes will be broaden and deepen our knowledge of
  45   plant defense mechanism.</p>
  46
  47   <p class="footnote"><a href="/static_content/community/feature/200405-2.jpg"><img src=
  48   "/static_content/community/feature/200405-2-small.jpg" width="700" height="694" border="0" alt=
  49   "Figure 1 - Hot pepper 5K cDNA microarray image" /></a><br />
  50   <strong>Figure 1.</strong> Hot pepper 5K cDNA microarray image.
  51   This image was captured by the Axon 4000A scanner after
  52   hybridization with reference (Cy-3 labeled, 1mM MgCl2) and
  53   treatment (Cy-5 labeled, Xag infiltration).</p>
  54
  55   <p class="footnote"><img src="/static_content/community/feature/200405-3.jpg" width="640" height=
  56   "480" border="0" alt=
  57   "Figure 2 - Picture of VIGS (Virus Induced Gene Silencing) plant" /><br />
  58
  59   <strong>Figure 2.</strong> Picture of VIGS (Virus Induced Gene
  60   Silencing) plant. The partial sequences of PDS (Phytoene
  61   desaturase) gene was inserted into modified TRV (Tobacco Rattle
  62   Virus) vector (a kind gift from Dr. Dinesh- Kumar) and then
  63   inoculated into hot pepper plant with Agrobacterium. The
  64   distinctive photo-bleaching phenotype was shown after 21 days
  65   after inoculation.</p>
  66
  67   <h2>Contact</h2>
  68
  69   <p><strong>Dr. Doil Choi</strong><br />
  70   Plant Genomics Laboratory, Genome Research Center,<br />
  71   Korea Research Institute of Bioscience &amp; Biotechnology
  72   (KRIBB)<br />
  73   52 Oun-dong, Yusong-gu, Taejon, 305-333, Korea<br />
  74   <br />
  75   Tel : +82-42-860-4340<br />
  76   Fax : +82-42-860-4309<br />
  77   e-mail : <a href=
  78   "mailto:doil\@kribb.re.kr">doil\@kribb.re.kr</a><br />
  79   relevant web-site :<br />
  80   <a href=
  81   "http://plant.pdrc.re.kr:7777/index.html">http://plant.pdrc.re.kr:7777/index.html</a><br />
  82
  83   <a href=
  84   "http://plant.pdrc.re.kr/ks200201/pepper.html">http://plant.pdrc.re.kr/ks200201/pepper.html</a><br />
  85
  86   <a href=
  87   "http://www.sgn.cornell.edu/content/sgn_data.pl">http://www.sgn.cornell.edu/content/sgn_data.pl</a><br />
  88
  89   <a href=
  90   "http://ted.bti.cornell.edu/pepper/">http://ted.bti.cornell.edu/pepper/</a><br />
  91   </p>
  92 END_HEREDOC
  93 $page->footer();