improve debug output of 404 errors
[sgn.git] / cgi-bin / community / feature / 200507.pl
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1 use strict;
2 use CXGN::Page;
3 my $page=CXGN::Page->new('200507.html','html2pl converter');
4 my $stylesheet=<<END_STYLESHEET;
5 <style type="text/css">
6 <!--
7 body {
8 color: #000000;
9 background-color: #ffffff;
12 p {
13 margin-left: 40px;
14 text-align: justify;
17 .footnote {
18 font-size: small;
19 /*width: 700px;*/
20 text-align:center;
23 .bibliography {
24 text-indent: -20px;
26 -->
27 </style>
28 END_STYLESHEET
30 $page->header('Coffee Biotechnology Lab', undef, $stylesheet);
31 print<<END_HEREDOC;
33 <center>
34 <h1>Coffee Biotechnology Lab</h1>
35 </center>
37 <p class="footnote"><img src="/static_content/community/feature/200507-1.jpg" border="0" width="800" height="572" alt="Coffee Lab" /><br />
38 <u>Coffee Biotech Group</u> - <strong>Front row:</strong> Adalgisa Soares, Julieta Almeida, Milene Silvestrini, Fernanda Pinto, Crisitana Pezzopane and
39 <br />Mirian Maluf. <strong>Back row:</strong> Bernadete Silvarolla, Julio Mistro, Marcos Brandalise, Ant&ocirc;nio Bai&atilde;o.</p>
41 <p class="footnote" style="float:right; width:375px; text-align:center;"><img src="/static_content/community/feature/200507-2.jpg" border="0" width="350" height="263" alt="Coffea arabica cultivars exhibiting red fruits" /><br />
42 Coffea arabica cultivars exhibiting red fruits
43 </p>
45 <p>
46 The Coffee Biotechnology Lab is a small (but increasing) group interested
47 in functional analysis of coffee genes related to agronomic
48 characteristics. The group works in a close association with the IAC
49 Coffee Breeding Program. During the past seventy years, this program has
50 been responsible for the development of several Coffea arabica and Coffea
51 canephora cultivars, which are planted extensively over Brazilian coffee
52 regions. In addition to coffee cultivars, there are numerous populations
53 under selection segregating for important agronomic traits, such as
54 resistance to abiotic and biotic stress, flowering and fruit development
55 timing, biochemical composition of fruits and seeds, and cup quality.
56 Also, the IAC has a comprehensive in situ Germplasm Collection, which
57 includes 22 different Coffea species.
58 </p>
60 <p>
61 The main limitation for coffee breeding is the very narrow genetic base
62 of the species C. arabica. Major efforts concentrate on developing
63 reliable tools for improving selection methods and also for introducing
64 novel traits into coffee cultivars.
65 </p>
67 <p class="footnote" style="float:left; width:375px; text-align:center;"><img src="/static_content/community/feature/200507-3.jpg" border="0" width="350" height="263" alt="Coffea arabica cultivars exhibiting yellow fruits" /><br />
68 Coffea arabica cultivars exhibiting yellow fruits
69 </p>
71 <p>
72 The Coffee Biotechnology Lab is responsible for the identification of
73 molecular markers suitable for marker-assisted selection. RAPD, AFLP and
74 microsattelites methods are used both to identify markers associated with
75 agronomic traits and to evaluate overall genetic variability of the Coffea
76 Germplasm Collection.
77 </p>
79 <p>
80 Recently, with the conclusion of the Coffee ESTs Genome sequencing, we
81 have initiated functional analysis of specific genes related mainly to
82 pathogen defense, flower development and caffeine biosynthesis. We use
83 methodologies that integrate expression profile analysis, such as RT-PCR,
84 differential display and microarrays, and traditional breeding methods. In
85 collaboration with the Biology Institute/UNESP, we have a project to
86 isolate
87 tissue-specific promoters from roots, leaves, and fruits. Also, in
88 collaboration with the IAC Cytogenetic Lab, we are mapping selected
89 microsattelites markers through FISH analysis.
90 </p>
92 <p>
93 Genomic research in coffee species is still very modest. However, due to
94 the economic and social importance of coffee culture and to the increasing
95 interest of research groups on coffee issues, we believe (and hope) that in
96 a short period this gap of genomic knowledge will be overcome.
97 </p><br clear="all" />
99 <div style="float:left; width:250; text-align:left;">
100 <h2>Contact Information</h2>
102 Dr. Mirian Perez Maluf<br />
103 Scientific Researcher / Embrapa<br />
104 Coffee Center &quot;Alcides Carvalho&quot;<br />
105 Agronomic Institute<br />
106 Campinas - SP<br />
107 Brazil<br />
108 <a href= "mailto:maluf\@iac.sp.gov.br">maluf\@iac.sp.gov.br</a><br />
109 </p>
110 </div>
112 <p class="footnote" style="float:right; width:400px; text-align:center;"><img src="/static_content/community/feature/200507-4.jpg" border="0" width="375" height="166" alt="IAC Coffee cultivars planted at the Experimental Station" /><br />
113 IAC Coffee cultivars planted at the Experimental Station
114 </p><br clear="all" />
116 <h2>Selected Publications</h2>
118 <p class="bibliography">
119 Mondego, J.M.C., Guerreiro Filho, O., Bengtson, M.H., Drummond, R.D.,
120 Felix, J. M., Duarte, M.P., Ramiro, D.A., Maluf, M.P., Sogayar, M.C.,
121 Menossi, M. (2005) "Isolation and characterization of Coffea genes induced
122 during leaf coffee miner (Leucoptera coffeella) infestation ", Plant
123 Science, in press.
124 </p>
126 <p class="bibliography">
127 Maluf, M.P., Silvestrini, Ruggiero, M., L. M. de C., Guerreiro-Filho, O.,
128 Colombo, C. A. (2005) &quot;Genetic diversity of cultivated Coffea arabica lines
129 assessed by RAPD, AFLP and SSR marker systems&quot;, Scientia Agricola, in
130 press.
131 </p>
133 <p class="bibliography">
134 Silvarolla, M.B., Mazzafera, P., Fazuoli, L.C. (2004) A naturally
135 decaffeinated arabica coffee. Nature, Inglaterra, v. 429, p. 826-826.
136 </p>
138 <p class="bibliography">
139 Mistro, J.C.; Fazuoli, L.C. ; Gon&ccedil;alves, P.S. ; Guerreiro-Filho, O. (2004).
140 Estimatives of genetic parameters and expected genetic gains with selection
141 in robust coffee. Crop Breeding And Applied Biotechnology, Vi&ccedil;osa (MG), v.
142 4, n. 1, p. 86-91.
143 </p>
145 <p class="bibliography">
146 Guerreiro Filho, O.; Mazzafera, P. (2003). Caffeine and resistance of
147 coffee to the berry borer Hypothenemus hampei (Coleoptera: Scolytidae).
148 Journal of Agricultural and Food Chemistry, Davis, Calif&oacute;rnia, EUA, v. 51,
149 n. 24, p. 6987-6991.
150 </p>
152 <p class="bibliography">
153 Aguiar, A.T., Maluf, M.P., Gallo, P.B., Fazuoli, L.C., Mori, E.E.M.,
154 Guerreiro-Filho, O. Technological and morphological characterization of
155 coffee Commercial lines developed by IAC - Brazil (2001). In: Association
156 Scientifique International du Caf&eacute; - 19 &eacute;me Colloque, Trieste. Association
157 Scientifique International du Caf&eacute;. 2001. v. CD-ROM
158 </p>
160 <p class="bibliography">
161 Orsi, C. H.; Colombo, Carlos A; Guerreiro-Filho, Oliveiro; Maluf, Mirian
162 Perez. Putative NBS-LRR resistance genes analogs (RGA) identified in
163 different species of the genera Coffea. (2001) In: 47o. Congresso nacional
164 de Gen&eacute;tica, &Aacute;guas de Lind&oacute;ia. Sociedade Brasileira de Gen&eacute;tica /Anais do
165 47o. Congresso Nacional de Gen&eacute;tica.
166 </p>
168 <p class="bibliography">
169 Pinto-Maglio, C.A.F; Barbosa, RL; Cuellar, T.; Maluf, M.P.; Pierozzi, N.I.;
170 Silvarolla, M.B.; Orsi, C.H. (2001) Chromosome characterization in Coffea
171 arabica L. using cytomolecular techniques. In: 14th International
172 Chromosome Conference, Wurzburg, Germany. Chromosome Research. v. 9, p. 100-
173 100.
174 </p>
176 END_HEREDOC
177 $page->footer();