cgi-bin/community/feature/200507.pl

   1 use strict;
   2 use CXGN::Page;
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  30 $page->header('Coffee Biotechnology Lab', undef, $stylesheet);
  31 print<<END_HEREDOC;
  32
  33 <center>
  34 <h1>Coffee Biotechnology Lab</h1>
  35 </center>
  36
  37 <p class="footnote"><img src="/static_content/community/feature/200507-1.jpg" border="0" width="800" height="572" alt="Coffee Lab" /><br />
  38 <u>Coffee Biotech Group</u> - <strong>Front row:</strong> Adalgisa Soares, Julieta Almeida, Milene Silvestrini, Fernanda Pinto, Crisitana Pezzopane and
  39 <br />Mirian Maluf.  <strong>Back row:</strong> Bernadete Silvarolla, Julio Mistro, Marcos Brandalise, Ant&ocirc;nio Bai&atilde;o.</p>
  40
  41 <p class="footnote" style="float:right; width:375px; text-align:center;"><img src="/static_content/community/feature/200507-2.jpg" border="0" width="350" height="263" alt="Coffea arabica cultivars exhibiting red fruits" /><br />
  42 Coffea arabica cultivars exhibiting red fruits
  43 </p>
  44
  45 <p>
  46 The Coffee Biotechnology Lab is a small (but increasing) group interested
  47 in functional analysis of coffee genes related to agronomic
  48 characteristics.  The group works in a close association with the IAC
  49 Coffee Breeding Program.  During the past seventy years, this program has
  50 been responsible for the development of several Coffea arabica and Coffea
  51 canephora cultivars, which are planted extensively over Brazilian coffee
  52 regions.  In addition to coffee cultivars, there are numerous populations
  53 under selection segregating for important agronomic traits, such as
  54 resistance to abiotic and biotic stress, flowering and fruit development
  55 timing, biochemical composition of fruits and seeds, and cup quality.
  56 Also, the IAC has a comprehensive in situ Germplasm Collection, which
  57 includes 22 different Coffea species.
  58 </p>
  59
  60 <p>
  61 The main limitation for coffee breeding is the very narrow genetic base
  62 of the species C. arabica.  Major efforts concentrate on developing
  63 reliable tools for improving selection methods and also for introducing
  64 novel traits into coffee cultivars.
  65 </p>
  66
  67 <p class="footnote" style="float:left; width:375px; text-align:center;"><img src="/static_content/community/feature/200507-3.jpg" border="0" width="350" height="263" alt="Coffea arabica cultivars exhibiting yellow fruits" /><br />
  68 Coffea arabica cultivars exhibiting yellow fruits
  69 </p>
  70
  71 <p>
  72 The Coffee Biotechnology Lab is responsible for the identification of
  73 molecular markers suitable for marker-assisted selection.  RAPD, AFLP and
  74 microsattelites methods are used both to identify markers associated with
  75 agronomic traits and to evaluate overall genetic variability of the Coffea
  76 Germplasm Collection.
  77 </p>
  78
  79 <p>
  80  Recently, with the conclusion of the Coffee ESTs Genome sequencing, we
  81 have initiated functional analysis of specific genes related mainly to
  82 pathogen defense, flower development and caffeine biosynthesis.  We use
  83 methodologies that integrate expression profile analysis, such as RT-PCR,
  84 differential display and microarrays, and traditional breeding methods.  In
  85 collaboration with the Biology Institute/UNESP, we have a project to
  86 isolate
  87 tissue-specific promoters from roots, leaves, and fruits.  Also, in
  88 collaboration with the IAC Cytogenetic Lab, we are mapping selected
  89 microsattelites markers through FISH analysis.
  90 </p>
  91
  92 <p>
  93  Genomic research in coffee species is still very modest. However, due to
  94 the economic and social importance of coffee culture and to the increasing
  95 interest of research groups on coffee issues, we believe (and hope) that in
  96 a short period this gap of genomic knowledge will be overcome.
  97 </p><br clear="all" />
  98
  99 <div style="float:left; width:250; text-align:left;">
 100 <h2>Contact Information</h2>
 101 <p>
 102 Dr. Mirian Perez Maluf<br />
 103 Scientific Researcher / Embrapa<br />
 104 Coffee Center &quot;Alcides Carvalho&quot;<br />
 105 Agronomic Institute<br />
 106 Campinas - SP<br />
 107 Brazil<br />
 108 <a href= "mailto:maluf\@iac.sp.gov.br">maluf\@iac.sp.gov.br</a><br />
 109 </p>
 110 </div>
 111
 112 <p class="footnote" style="float:right; width:400px; text-align:center;"><img src="/static_content/community/feature/200507-4.jpg" border="0" width="375" height="166" alt="IAC Coffee cultivars planted at the Experimental Station" /><br />
 113 IAC Coffee cultivars planted at the Experimental Station
 114 </p><br clear="all" />
 115
 116 <h2>Selected Publications</h2>
 117
 118 <p class="bibliography">
 119 Mondego, J.M.C.,  Guerreiro  Filho,  O.,  Bengtson,  M.H.,  Drummond,  R.D.,
 120 Felix, J. M., Duarte,  M.P.,  Ramiro,  D.A.,  Maluf,  M.P.,  Sogayar,  M.C.,
 121 Menossi, M. (2005) "Isolation and characterization of Coffea  genes  induced
 122 during  leaf  coffee  miner  (Leucoptera  coffeella)  infestation  ",  Plant
 123 Science, in press.
 124 </p>
 125
 126 <p class="bibliography">
 127 Maluf, M.P., Silvestrini, Ruggiero, M., L. M. de C., Guerreiro-Filho, O.,
 128 Colombo, C. A.  (2005) &quot;Genetic diversity of cultivated Coffea arabica lines
 129 assessed by RAPD, AFLP and SSR marker systems&quot;, Scientia Agricola, in
 130 press.
 131 </p>
 132
 133 <p class="bibliography">
 134 Silvarolla,  M.B.,  Mazzafera,  P.,  Fazuoli,  L.C.   (2004)   A   naturally
 135 decaffeinated arabica coffee. Nature, Inglaterra, v. 429, p. 826-826.
 136 </p>
 137
 138 <p class="bibliography">
 139 Mistro, J.C.; Fazuoli, L.C. ; Gon&ccedil;alves, P.S. ; Guerreiro-Filho, O.  (2004).
 140 Estimatives of genetic parameters and expected genetic gains with  selection
 141 in robust coffee. Crop Breeding And Applied Biotechnology, Vi&ccedil;osa  (MG),  v.
 142 4, n. 1, p. 86-91.
 143 </p>
 144
 145 <p class="bibliography">
 146 Guerreiro Filho, O.;  Mazzafera,  P.  (2003).  Caffeine  and  resistance  of
 147 coffee to the berry  borer  Hypothenemus  hampei  (Coleoptera:  Scolytidae).
 148 Journal of Agricultural and Food Chemistry, Davis, Calif&oacute;rnia, EUA,  v.  51,
 149 n. 24, p. 6987-6991.
 150 </p>
 151
 152 <p class="bibliography">
 153 Aguiar, A.T.,  Maluf,  M.P.,  Gallo,  P.B.,  Fazuoli,  L.C.,  Mori,  E.E.M.,
 154 Guerreiro-Filho, O.  Technological  and  morphological  characterization  of
 155 coffee Commercial lines developed by IAC - Brazil  (2001).  In:  Association
 156 Scientifique International du Caf&eacute; - 19 &eacute;me Colloque,  Trieste.  Association
 157 Scientifique International du Caf&eacute;. 2001. v. CD-ROM
 158 </p>
 159
 160 <p class="bibliography">
 161 Orsi, C. H.; Colombo, Carlos A;  Guerreiro-Filho,  Oliveiro;  Maluf,  Mirian
 162 Perez.  Putative  NBS-LRR  resistance  genes  analogs  (RGA)  identified  in
 163 different species of the genera Coffea. (2001) In: 47o.  Congresso  nacional
 164 de Gen&eacute;tica, &Aacute;guas de Lind&oacute;ia. Sociedade Brasileira de  Gen&eacute;tica  /Anais  do
 165 47o. Congresso Nacional de Gen&eacute;tica.
 166 </p>
 167
 168 <p class="bibliography">
 169 Pinto-Maglio, C.A.F; Barbosa, RL; Cuellar, T.; Maluf, M.P.; Pierozzi,  N.I.;
 170 Silvarolla, M.B.; Orsi, C.H. (2001) Chromosome  characterization  in  Coffea
 171 arabica  L.  using  cytomolecular   techniques.   In:   14th   International
 172 Chromosome Conference, Wurzburg, Germany. Chromosome Research. v. 9, p. 100-
 173 100.
 174 </p>
 175
 176 END_HEREDOC
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