cgi-bin/content/libraries/mixedelicitor.pl

   1 use strict;
   2 use CXGN::Page;
   3 my $page=CXGN::Page->new('mixedelicitor.html','html2pl converter');
   4 $page->header('Library of Leaf Tissue Under Mixed Elicitor
   5             Stress');
   6 print<<END_HEREDOC;
   7
   8   <center>
   9
  10     <table summary="" width="720" cellpadding="0" cellspacing="0"
  11     border="0">
  12       <tr>
  13         <td>
  14           <center>
  15             <h2>Library of Leaf Tissue Under Mixed Elicitor
  16             Stress</h2>
  17           </center>
  18
  19           <table summary="" width="100\%" cellspacing="5">
  20             <tr valign="top">
  21               <td width="20\%">Library:</td>
  22
  23               <td>cLET</td>
  24             </tr>
  25
  26             <tr valign="top">
  27               <td>TIGR ID:</td>
  28
  29               <td>TME</td>
  30             </tr>
  31
  32             <tr valign="top">
  33               <td>Authors:</td>
  34
  35               <td>Tishina Subrahmanyam and Gregory Martin</td>
  36             </tr>
  37
  38             <tr valign="top">
  39               <td>Date made:</td>
  40
  41               <td>07/99</td>
  42             </tr>
  43
  44             <tr valign="top">
  45               <td>Species:</td>
  46
  47               <td><em>Lycopersicon esculentum</em></td>
  48             </tr>
  49
  50             <tr valign="top">
  51               <td>Accession:</td>
  52
  53               <td>Rio Grande PtoR</td>
  54             </tr>
  55
  56             <tr valign="top">
  57               <td>Tissue:</td>
  58
  59               <td>leaf</td>
  60             </tr>
  61
  62             <tr valign="top">
  63               <td>Developmental stage:</td>
  64
  65               <td>4-6 week old plants</td>
  66             </tr>
  67
  68             <tr valign="top">
  69               <td>Vector:</td>
  70
  71               <td>pBluescript SK(+/-)</td>
  72             </tr>
  73
  74             <tr valign="top">
  75               <td>Host:</td>
  76
  77               <td>SOLR</td>
  78             </tr>
  79
  80             <tr valign="top">
  81               <td>Primary pfu:</td>
  82
  83               <td>1.0 x 10<small><sup>6</sup></small></td>
  84             </tr>
  85
  86             <tr valign="top">
  87               <td>Number mass excised:</td>
  88
  89               <td>1.0 x 10<small><sup>8</sup></small></td>
  90             </tr>
  91
  92             <tr valign="top">
  93               <td>Average insert length:</td>
  94
  95               <td>1.0 Kb</td>
  96             </tr>
  97
  98             <tr valign="top">
  99               <td>Cloning sites:</td>
 100
 101               <td>5' EcoRI, 3' XhoI</td>
 102             </tr>
 103
 104             <tr valign="top">
 105               <td>Antibiotic:</td>
 106
 107               <td>ampicillin</td>
 108             </tr>
 109
 110             <tr valign="top">
 111               <td>Primers:</td>
 112
 113               <td>M13F and M13R</td>
 114             </tr>
 115
 116             <tr valign="top">
 117               <td>Comments:</td>
 118
 119               <td>The EcoRI site was destroyed during cloning. Use
 120               any enzyme to the left of it along with XhoI to cut
 121               out the insert. Also note that the library was
 122               amplified prior to mass excision. 11,520 clones were
 123               picked into 30 384-well plates. In the following
 124               table, BTH stands for the chemical benzothiadiazole,
 125               which induces SAR in many dicot species. EIX is
 126               ethylene inducing xylanase from the fungus
 127               <em>Trichoderma</em>. JA is jasmonic acid.</td>
 128             </tr>
 129           </table>
 130
 131           <table summary="" width="100\%" border="1" cellspacing="5"
 132           cellpadding="5">
 133             <tr>
 134               <td><strong>Biotic/abiotic elicitor</strong></td>
 135               <td><strong>Method of treatment</strong></td>
 136               <td><strong>Concentration</strong></td>
 137               <td><strong>Time points (hours)</strong></td>
 138               <td><strong>Has the treatment worked?</strong></td>
 139             </tr>
 140
 141             <tr>
 142               <td>INA (2,6 dichloroisonicotinic acid)</td>
 143               <td>(1)foliar spray application (2)Petiole immersion
 144               - treat in solution for 30 minutes, then transfer to
 145               water</td>
 146               <td>0.5 mM</td>
 147               <td>4, 24</td>
 148               <td>RNA gel blot analysis - probe with osmotin</td>
 149             </tr>
 150
 151             <tr>
 152               <td>BTH</td>
 153
 154               <td>(1)foliar spray application (2)Petiole immersion
 155               - treat in solution for 30 minutes, then transfer to
 156               water</td>
 157
 158               <td>1.5 mM</td>
 159
 160               <td>4, 24</td>
 161
 162               <td>RNA gel blot analysis - probe with basic
 163               glucanase</td>
 164             </tr>
 165
 166             <tr>
 167               <td>JA</td>
 168
 169               <td>petiole immersion in closed chamber
 170               incubation</td>
 171
 172               <td>100 uM</td>
 173
 174               <td>4, 12</td>
 175
 176               <td>RNA gel blot analysis - probe with pin gene</td>
 177             </tr>
 178
 179             <tr>
 180               <td>ethylene</td>
 181
 182               <td>closed chamber incubation</td>
 183
 184               <td>50 ppm of gas injected into chamber</td>
 185
 186               <td>2, 12</td>
 187
 188               <td>plants showed epinasty - leaf curl and bending
 189               downwards</td>
 190             </tr>
 191
 192             <tr>
 193               <td>fenthion</td>
 194
 195               <td>vacuum infiltration</td>
 196
 197               <td>0.001\% Silwet</td>
 198
 199               <td>4, 12</td>
 200
 201               <td>fenthion cell death observed 11 hours after
 202               infiltration</td>
 203             </tr>
 204
 205             <tr>
 206               <td>EIX</td>
 207
 208               <td>vacuum infiltration</td>
 209
 210               <td>1 ug/ml solution</td>
 211
 212               <td>8, 48</td>
 213
 214               <td>small lesions visible 48 hours after
 215               infiltration</td>
 216             </tr>
 217
 218             <tr>
 219               <td>okadaic acid</td>
 220
 221               <td>transpiration stream for 45 minutes, then
 222               transfer to dd water</td>
 223
 224               <td>1 uM</td>
 225
 226               <td>4, 12</td>
 227
 228               <td>wilting</td>
 229             </tr>
 230
 231             <tr>
 232               <td>systemin</td>
 233
 234               <td>petiole immersion, then transfer to dd water</td>
 235
 236               <td>90 ul of 1 nM solution in sodium phosphate
 237               buffer</td>
 238
 239               <td>4, 12</td>
 240
 241               <td>RNA gel blot analysis - probe with pin gene</td>
 242             </tr>
 243           </table>
 244         </td>
 245       </tr>
 246     </table>
 247
 248   </center>
 249 END_HEREDOC
 250 $page->footer();