cgi-bin/content/libraries/ovary.pl

   1 use strict;
   2 use CXGN::Page;
   3 my $page=CXGN::Page->new('ovary.html','html2pl converter');
   4 $page->header('Ovary Library');
   5 print<<END_HEREDOC;
   6
   7   <center>
   8
   9     <table summary="" width="720" cellpadding="0" cellspacing="0"
  10     border="0">
  11       <tr>
  12         <td>
  13           <center>
  14             <h2>Ovary Library</h2>
  15           </center>
  16
  17           <table summary="" width="100\%" cellspacing="5">
  18             <tr valign="top">
  19               <td width="20\%">Library:</td>
  20
  21               <td>cLED</td>
  22             </tr>
  23
  24             <tr valign="top">
  25               <td>TIGR ID:</td>
  26
  27               <td>TOV</td>
  28             </tr>
  29
  30             <tr valign="top">
  31               <td>Authors:</td>
  32
  33               <td>Alcala, Vrebalov, White, and Giovannoni</td>
  34             </tr>
  35
  36             <tr valign="top">
  37               <td>Date made:</td>
  38
  39               <td>11/98</td>
  40             </tr>
  41
  42             <tr valign="top">
  43               <td>Species:</td>
  44
  45               <td><em>Lycopersicon esculentum</em></td>
  46             </tr>
  47
  48             <tr valign="top">
  49               <td>Accession:</td>
  50
  51               <td>TA496</td>
  52             </tr>
  53
  54             <tr valign="top">
  55               <td>Tissue:</td>
  56
  57               <td>carpel</td>
  58             </tr>
  59
  60             <tr valign="top">
  61               <td>Developmental stage:</td>
  62
  63               <td>5 days pre-anthesis to 5 days post-anthesis</td>
  64             </tr>
  65
  66             <tr valign="top">
  67               <td>Vector:</td>
  68
  69               <td>pBluescript SK(+/-)</td>
  70             </tr>
  71
  72             <tr valign="top">
  73               <td>Host:</td>
  74
  75               <td>SOLR</td>
  76             </tr>
  77
  78             <tr valign="top">
  79               <td>Primary pfu:</td>
  80
  81               <td>1.0 x 10<small><sup>6</sup></small></td>
  82             </tr>
  83
  84             <tr valign="top">
  85               <td>Number mass excised:</td>
  86
  87               <td>3.0 x 10<small><sup>5</sup></small></td>
  88             </tr>
  89
  90             <tr valign="top">
  91               <td>Average insert length:</td>
  92
  93               <td>1.5 Kb</td>
  94             </tr>
  95
  96             <tr valign="top">
  97               <td>Cloning sites:</td>
  98
  99               <td>5' EcoRI, 3' XhoI</td>
 100             </tr>
 101
 102             <tr valign="top">
 103               <td>Antibiotic:</td>
 104
 105               <td>ampicillin</td>
 106             </tr>
 107
 108             <tr valign="top">
 109               <td>Primers:</td>
 110
 111               <td>M13F and M13R</td>
 112             </tr>
 113
 114             <tr valign="top">
 115               <td>Comments:</td>
 116
 117               <td>Construction of an early carpel (pre-anthesis
 118               through 5 days post-anthesis) cDNA library has been
 119               completed. Approximately one millions unamplified
 120               clones were generated and 300,000 were used in mass
 121               excision. Forty 384-well plates (15,360 clones) were
 122               picked. On hundred random clones were miniprepped and
 123               PCR'd with an average insert size of 1.5 Kb (range
 124               0.8-2.5 Kb). XhoI and EcoRI digests of the same 100
 125               clones were performed. Only two clones were
 126               identified with internal XhoI and EcoRI sites, which
 127               may be indicative of chimeric ligation, though double
 128               digestion suggested they were not chimeras (the
 129               internal sites are both at least several hundred bp
 130               internal, but not adjacent). 48 of the clones were
 131               sequenced.Flowers were collected from about 25 TA496
 132               plants every two days for one month. Flowers were
 133               approximately 5 days pre-anthesis (based on tagging)
 134               through 5 days post-anthesis (based on tagging and
 135               appearance). At 5 dpa, the carpels were about 0.5 cm
 136               in diameter. Flowers were collected at five different
 137               stages: 1)5 days pre-anthesis, 2) anthesis (based on
 138               partial sepal separation and anther tube swelling, 3)
 139               5 dpa, 4) intermediate between 1 and 2, and 5)
 140               intermediate between 2 and 3. About 0.5 grams of
 141               tissue was collected from hundreds of flowers in the
 142               early stages, while about 10 grams of tissue was
 143               collected from about 10 stage-5 carpels. 0.5 to 1
 144               gram per stage was combined and used for RNA
 145               isolation. The carpels included ovules and embryos.
 146               The anthers from the first three stages and some of
 147               the fourth stage were collected for an anther
 148               library.</td>
 149             </tr>
 150           </table>
 151         </td>
 152       </tr>
 153     </table>
 154
 155   </center>
 156 END_HEREDOC
 157 $page->footer();