cgi-bin/content/libraries/quiescentseed.pl

   1 use strict;
   2 use CXGN::Page;
   3 my $page=CXGN::Page->new('quiescentseed.html','html2pl converter');
   4 $page->header('Quiescent Seed Library');
   5 print<<END_HEREDOC;
   6
   7   <center>
   8
   9     <table summary="" width="720" cellpadding="0" cellspacing="0"
  10     border="0">
  11       <tr>
  12         <td>
  13           <center>
  14             <h2>Quiescent Seed Library</h2>
  15           </center>
  16
  17           <table summary="" width="100\%" cellspacing="5">
  18             <tr valign="top">
  19               <td width="20\%">Library:</td>
  20
  21               <td>cLEE</td>
  22             </tr>
  23
  24             <tr valign="top">
  25               <td>TIGR ID:</td>
  26
  27               <td>TSE</td>
  28             </tr>
  29
  30             <tr valign="top">
  31               <td>Authors:</td>
  32
  33               <td>Alcala, Vrebalov, White, and Giovannoni</td>
  34             </tr>
  35
  36             <tr valign="top">
  37               <td>Date made:</td>
  38
  39               <td>12/99</td>
  40             </tr>
  41
  42             <tr valign="top">
  43               <td>Species:</td>
  44
  45               <td><em>Lycopersicon esculentum</em></td>
  46             </tr>
  47
  48             <tr valign="top">
  49               <td>Accession:</td>
  50
  51               <td>TA496</td>
  52             </tr>
  53
  54             <tr valign="top">
  55               <td>Tissue:</td>
  56
  57               <td>seeds</td>
  58             </tr>
  59
  60             <tr valign="top">
  61               <td>Developmental stage:</td>
  62
  63               <td>dissected five days post-anthesis to fruit
  64               over-ripe stage</td>
  65             </tr>
  66
  67             <tr valign="top">
  68               <td>Vector:</td>
  69
  70               <td>pBluescript SK(+/-)</td>
  71             </tr>
  72
  73             <tr valign="top">
  74               <td>Host:</td>
  75
  76               <td>SOLR</td>
  77             </tr>
  78
  79             <tr valign="top">
  80               <td>Primary pfu:</td>
  81
  82               <td>1.2 x 10<small><sup>4</sup></small></td>
  83             </tr>
  84
  85             <tr valign="top">
  86               <td>Number mass excised:</td>
  87
  88               <td>1.2 x 10<small><sup>4</sup></small></td>
  89             </tr>
  90
  91             <tr valign="top">
  92               <td>Average insert length:</td>
  93
  94               <td>1.0 Kb</td>
  95             </tr>
  96
  97             <tr valign="top">
  98               <td>Cloning sites:</td>
  99
 100               <td>5' EcoRI, 3' XhoI</td>
 101             </tr>
 102
 103             <tr valign="top">
 104               <td>Antibiotic:</td>
 105
 106               <td>ampicillin</td>
 107             </tr>
 108
 109             <tr valign="top">
 110               <td>Primers:</td>
 111
 112               <td>M13F and M13R</td>
 113             </tr>
 114
 115             <tr valign="top">
 116               <td>Comments:</td>
 117
 118               <td>All of the primary library was mass excised.
 119               Tissue is from seed and maturing carpels. Fruit from
 120               5 dpa, 10 dpa, 20 dpa, mature green, breaker, firm,
 121               red-ripe, and very soft, red-ripe were collected.
 122               Seed was removed from all stages, though there was
 123               relatively little from the 5 dpa stage. 0.1 g of 5
 124               dpa seed was combined with 0.3 g of 10 dpa, 0.7 g of
 125               20 dpa, and 0.3 g from each of the mature green
 126               through late ripening stage seeds (1.2 g total for
 127               these 4) for RNA extraction.</td>
 128             </tr>
 129           </table>
 130         </td>
 131       </tr>
 132 </table>
 133
 134   </center>
 135 END_HEREDOC
 136 $page->footer();