3 my $page=CXGN
::Page
->new('tomatoredripe.html','html2pl converter');
4 $page->header('cLEN/TRR - Tomato red ripe');
9 <table summary="" width="720" cellpadding="0" cellspacing="0"
14 <h2>cLEN/TRR - Tomato red ripe</h2>
17 <table summary="" width="100\%" cellspacing="5">
19 <td width="20\%">Library:</td>
30 <td>Alcala, Vrebalov, White, and Giovannoni</td>
40 <td><em>Lycopersicon esculentum</em></td>
50 <td>fruit pericarp</td>
54 <td>Developmental stage:</td>
55 <td>ripe/over-ripe</td>
60 <td>pBluescript SK(-)</td>
70 <td>1.5 x 106<small><sup>6</sup></small></td>
74 <td>Number mass excised:</td>
75 <td>2.0 x 105<small><sup>6</sup></small></td>
79 <td>Average insert length:</td>
84 <td>Cloning sites:</td>
85 <td>5' EcoRI, 3' XhoI</td>
100 <td>Fruit were tagged at the "breaker" stage (first sign of lycopene accumulation
101 on the blossom end of the fruit) and harvested 7 days post-breaker (fully red-ripe),
102 10 days post breaker, and 20 days post-breaker (over-ripe). 20 day fruit which
103 showed external or internal signs of pathogenesis were discarded. Fruit were cut
104 in half and the seeds and locules were discarded prior to freezing the pericarp.</td>
109 <center><em>NOTE: 9/14/99 cLEN- 84 total sequences, 10 dupes, 88.1\% unique, with 40 unknowns.<br />
110 No junk was found (chloroplast, mitochondria, or E. coli).</em></center>