1 HindIII AAGCTT HindIII,10000u/$53
2 BamHI GGATCC BamHI,10000u/$53
3 EcoRI GAATTC EcoRI,10000u/$53 EcoRI/FunII
4 BsoBI C[C|T]CG[A|G]G BsoBI,10000u/$58 Ama87I/AvaI/BsiHKCI/BsoBI/Eco88I/NspIII/Nli3877I
5 PstI CTGCAG PstI,10000u/$58 BspMAI/PstI
6 HinfI GA.TC HinfI,5000u/$53
7 MspI CCGG MspI,5000u/$56 MspI/BsiSI/HapII/HpaII
8 PvuII CAGCTG PvuII,5000u/$53
9 BanI GG[C|T][A|G]CC BanI,5000u/$61 BanI/AccB1I/BshNI/BspT107I
10 ApaI GGGCCC ApaI,5000u/$63 Bsp120I/PspOMI/ApaI
11 XhoI CTCGAG XhoI,5000u/$63 XhoI/BssHI/PaeR7I/Sfr274I/SlaI/StrI/TliI/SciI
12 TaqI TCGA TaqI,4000u/$53 TaqI/EsaBC3I
13 EcoRV GATATC EcoRV,4000u/$53 EcoRV/Eco32I
14 KpnI GGTACC KpnI,4000u/$53 Acc65I/Asp718I/KpnI
15 BclI TGATCA BclI,3000u/$53 BclI/FbaI/Ksp22I
16 HaeIII GGCC HaeIII,3000u/$56 HaeIII/BshFI/BspANI/BsuRI/PalI/PhoI
17 XbaI TCTAGA XbaI,3000u/$63
18 StyI CC[T|A][T|A]GG StyI,3000u/$63 StyI/BssT1I/Eco130I/EcoT14I/ErhI
19 BstBI TTCGAA BstBI,2500u/$53 BstBI/AsuII/Bpu14I/Bsp119I/BspT104I/Csp45I/NspV/SfuI
20 ApaLI GTGCAC ApaLI,2500u/$58 ApaLI/Alw44I/VneI
21 BglII AGATCT BglII,2000u/$53
22 SmaI CCCGGG SmaI,2000u/$56 Cfr9I/PspAI/XmaI/XmaCI/UthSI/SmaI
23 BglI GCC.....GGC BglI,2000u/$53
24 SalI GTCGAC SalI,2000u/$56
25 AflII CTTAAG AflII,2000u/$56 AflII/BfrI/BspTI/Bst98I/MspCI/Vha464I
26 AvaII GG[T|A]CC AvaII,2000u/$56 AvaII/VpaK11AI/Bme18I/Eco47I/SinI/VpaK11BI/Psp03I
27 BstEII GGT.ACC BstEII,2000u/$53 BstEII/BstPI/Eco91I/EcoO65I/PspEI
28 BstNI CC[T|A]GG BstNI,3000u/$53 BstNI/AjnI/EcoRII/Psp6I/PspGI/BptI/BseBI/BstOI/Bst2UI/MvaI
29 CfoI/HhaI GCGC HhaI,2000u/$53 CfoI/HhaI/Hin6I/HinP1I/HspAI/AspLEI/BstHHI
30 SacI GAGCTC SacI,2000u/$53 SacI/Ecl136II/EcoICRI/Psp124BI/SstI
31 SacII CCGCGG SacII,2000u/$56 SacII/Cfr42I/KspI/Sfr303I/SgrBI
32 SfiI GGCC.....GGCC SfiI,2000u/$61
33 DraI TTTAAA DraI,2000u/$58
34 EcoO109I [A|G]GG.CC[C|T] EcoO109I,2000u/$58 DraII/EcoO109I
35 AseI ATTAAT AseI,2000u/$58 AseI/PshBI/VspI
36 BanII G[A|G]GC[C|T]C BanII,2000u/$61 BanII/Eco24I/EcoT38I/FriOI
37 BsaBI GAT....ATC BsaBI,2000u/$61 BsaBI/Bse8I/BseJI/MamI
38 NciI CC[C|G]GG NciI,2000u/$58 NciI/EcoHI/AsuC2I/BcnI
39 PmlI CACGTG PmlI,2000u/$58 PmlI/AcvI/BbrPI/Eco72I/PmaCI/PspCI
40 SwaI ATTTAAAT SwaI,2000u/$61 SwaI/SmiI
41 BstUI CGCG BstUI,1000u/$53 BstUI/SelI/AccII/Bsh1236I/BstFNI/MvnI
42 BstYI [A|G]GATC[C|T] BstYI,2000u/$58 BstYI/BstX2I/MflI/PsuI/XhoII
43 HincII GT[C|T][A|G]AC HincII,1000u/$53 HincII/HindII
44 Sau96I GG.CC Sau96I,1000u/$56 Sau96I/UnbI/AspS9I/Cfr13I/PspPI/FmuI
45 StuI AGGCCT StuI,1000u/$56 StuI/AatI/Eco147I/PceI/SseBI
46 XmnI GAA....TTC XmnI,1000u/$56 XmnI/Asp700I/MroXI/PdmI
47 SsPI AATATT SsPI,1000u/$61
48 MluI ACGCGT MluI,1000u/$58
49 AluI AGCT AluI,1000u/$61
50 BspDI ATCGAT BspDI,2000u/$58 ClaI/BanIII/Bsa29I/BseCI/Bsp106I/BspDI/BspXI/Bsu15I/BsuTUI/ClaI/ZhoI
51 BslI CC.......GG BslI,1000u/$58 Bsc4I/BseLI/BsiYI/BslI
52 XcmI CCA.........TGG XcmI,1000u/$61
53 BstXI CCA......TGG BstXI,1000u/$58
54 SgrAI C[A|G]CCGG[C|T]G SgrAI,1000u/$61
55 BsiHKAI G[T|A]GC[T|A]C BsiHKAI,1000u/$58 Alw21I/AspHI/Bbv12I/BsiHKAI
56 Hpy188I TC.GA Hpy188I,1000u/$61
57 BsaHI G[A|G]CG[C|T]C BsaHI,2000u/$58 BsaHI/AcyI/BstACI/Hin1I/Hsp92I
58 BsiEI CG[A|G][C|T]CG BsiEI,1000u/$58 BsiEI/Bsh1285I/BstMCI
59 BspEI TCCGGA BspEI,1000u/$61 BspEI/AccIII/Aor13HI/BlfI/BseAI/Bsp13I/Kpn2I/MroI
60 BsrGI TGTACA BsrGI,1000u/$61 BsrGI/Bsp1407I/BstAUI/SspBI
61 BstZ17I GTATAC BstZ17I,1000u/$61 BstZ17I/BssNAI/Bst1107I/SnaI
62 BtgI CC[A|G][C|T]GG BtgI,1000u/$58 BstDSI/BtgI
63 DpnII GATC DpnII,1000u/$63 DpnII/BfuCI/Bsp143I/BstMBI/DpnI/Kzo9I/MboI/NdeII/Sau3AI/DpnI/BstKTI/ChaI
64 AdeI/DraIII CAC...GTG DraIII,1000u/$61 AdeI/DraIII
65 EcoNI CCT.....AGG EcoNI,1000u/$61 EcoNI/BstENI/XagI
66 NcoI CCATGG NcoI,1000u/$56 Bsp19I/NcoI
67 NgoMIV GCCGGC NgoMIV,1000u/$58 NgoMIV/MroNI/NaeI/PdiI
68 NheI GCTAGC NheI,1000u/$61 NheI/AsuNHI/BmtI
69 NruI TCGCGA NruI,1000u/$53 NruI/Bsp68I
70 NsiI ATGCAT NsiI,1000u/$58 NsiI/Ppu10I/BfrBI/EcoT22I/Mph1103I/Zsp2I/AvaIII
71 PflFI GAC...GTC PflFI,2000u/$58 AspI/PflFI/PsyI/TelI/Tth111I
72 PflMI CCA.....TGG PflMI,1000u/$58 PflMI/AccB7I/PflBI/Van91I
73 PshAI GAC....GTC PshAI,1000u/$61 PshAI/BoxI/BstPAI
74 RsaI GTAC RsaI,1000u/$53 RsaI/Csp6I/AfaI
75 ScaI AGTACT ScaI,1000u/$58 ScaI/AssI/ZrmI
76 ScrFI CC.GG ScrFI,1000u/$61 ScrFI/BssKI/BstSCI/StyD4I/Bme1390I/MspR9I
77 Tsp509I AATT Tsp509I,1000u/$58 Tsp509I/Sse9I/TasI/TspEI
79 ApoI [A|G]AATT[C|T] ApoI,1000u/$63 ApoI/AcsI/XapI
80 AasI/DrdI/DseDI GAC......GTC N/A
81 Acc16I/AviII/FspI/NsbI TGCGCA N/A
82 AccI/FblI/XmiI GT[C|A][T|G]AC N/A
83 AclI,Psp1406I AACGTT N/A
84 AfeI/Aor51HI/Eco47III/FunI AGCGCT N/A
85 AflIII AC[A|G][C|T]GT N/A
86 AgeI/AsiAI/BshTI/CspAI/PinAI ACCGGT N/A
87 AhdI/AspEI/DriI/Eam1105I/EclHKI GAC.....GTC N/A
88 AhlI/BcuI/SpeI ACTAGT N/A
89 AleI/OliI CAC....GTG N/A
90 AlwNI/CaiI CAG...CTG N/A
91 ApeKI/TseI GC[T|A]GC N/A
93 AsiSI/SgfI GCGATCGC N/A
94 AspA2I/AvrII/BlnI/XmaJI CCTAGG N/A
95 AxyI/Bse21I/Bsu36I/Eco81I CCT.AGG N/A
96 BalI/MlsI/MluNI/MscI/Msp20I TGGCCA N/A
97 BbuI/PaeI/SpaHI/SphI GCATGC N/A
98 BfaI/FspBI/MaeI/XspI CTAG N/A
99 BfmI/BpcI/BstSFI/SfcI CT[A|G][C|T]AG N/A
100 BlpI/Bpu1102I/Bsp1720I/CelII GCT.AGC N/A
101 Bme1580I/BseSI G[T|G]GC[C|A]C N/A
102 BmyI/Bsp1286I/MhlI/SduI G[A|T|G]GC[A|T|C]C N/A
103 BsaAI/BstBAI [C|T]ACGT[A|G] N/A
104 BsaJI/BseDI/BssECI CC..GG N/A
105 BsaWI [T|A]CCGG[T|A] N/A
106 Bse118I/BsrFI/BssAI/Cfr10I [A|G]CCGG[C|T] N/A
107 BsePI/BssHII/PauI GCGCGC N/A
108 BseX3I/BstZI/EagI/EclXI/Eco52I CGGCCG N/A
109 BsiWI/Pfl23II/PspLI/SunI CGTACG N/A
110 BspCI/MvrI/Ple19I/PvuI CGATCG N/A
111 BspHI/PagI/RcaI TCATGA N/A
112 BspLI/NlaIV/PspN4I GG..CC N/A
113 BspLU11I/PciI ACATGT N/A
114 Bst4CI/HpyCH4III/TaaI AC.GT N/A
115 BstAPI/ApaBI GCA.....TGC N/A
116 BstC8I/Cac8I GC..GC N/A
117 BstDEI/DdeI CT.AG N/A
118 BstMWI/HpyF10VI/MwoI GC.......GC N/A
119 BstNSI/NspI/XceI [A|G]CATG[C|T] N/A
120 BstSNI/Eco105I/SnaBI TACGTA N/A
121 CciNI/NotI GCGGCCGC N/A
122 CfrI/EaeI [C|T]GGCC[A|G] N/A
123 CpoI/CspI/RsrII/Rsr2I CGG[T|A]CCG N/A
124 CviJI [A|G]GC[C|T] N/A
125 FauNDI/NdeI CATATG N/A
126 Fnu4HI/Fsp4HI/ItaI/SatI/BthCI GC.GC N/A
128 FspAI [A|G]TGCGCA[C|T] N/A
129 HaeI [T|A]GGCC[T|A] N/A
130 HgiEII ACC......GGT N/A
131 HpaI/KspAI GTTAAC N/A
133 Hpy8I/MjaIV GT..AC N/A
135 HpyCH4IV/MaeII/TaiI/TscI ACGT N/A
137 KasI/Mly113I/NarI/EgeI/EheI/SfoI/BbeI GGCGCC N/A
138 LpnI/Bsp143II/BstH2I/HaeII [A|G]GCGC[C|T] N/A
139 MabI/SexAI ACC[T|A]GGT N/A
142 MseI/Tru1I/Tru9I TTAA N/A
143 MslI/SmiMI CA[C|T]....[A|G]TG N/A
144 MspA1I C[C|A]GC[T|G]G N/A
145 MssI/PmeI GTTTAAAC N/A
146 NlaIII/FatI/CviAII/Hin1II/Hsp92II CATG N/A
147 NmuCI/Tsp45I GT[C|G]AC N/A
150 PfeI/TfiI GA[T|A]TC N/A
152 PpuMI/PpuXI/Psp5II/PspPPI [A|G]GG[T|A]CC[C|T] N/A
154 PspXI [A|C|G]CTCGAG[C|T|G] N/A
155 SanDI GGG[T|A]CCC N/A
156 SbfI/SdaI/Sse8387I CCTGCAGG N/A
157 SmlI CT[C|T][A|G]AG N/A
160 Sse8647I AGG[T|A]CCT N/A
161 TatI [T|A]GTAC[T|A] N/A
163 UbaTI TGA......TCA N/A
164 ZraI/AatII GACGTC N/A