cgi-bin/content/libraries/pseudomonasresponse.pl

   1 use strict;
   2 use CXGN::Page;
   3 my $page=CXGN::Page->new('pseudomonasresponse.html','html2pl converter');
   4 $page->header('Library of Leaves From _Pseudomonas_ Resistant
   5             Variety');
   6 print<<END_HEREDOC;
   7
   8   <center>
   9
  10     <table summary="" width="720" cellpadding="0" cellspacing="0"
  11     border="0">
  12       <tr>
  13         <td>
  14           <center>
  15             <h2>Library of Leaves From <em>Pseudomonas</em> Resistant
  16             Variety</h2>
  17           </center>
  18
  19           <table summary="" width="100\%" cellspacing="5">
  20             <tr valign="top">
  21               <td width="20\%">Library:</td>
  22
  23               <td>cLER</td>
  24             </tr>
  25
  26             <tr valign="top">
  27               <td>TIGR ID:</td>
  28
  29               <td>TPR</td>
  30             </tr>
  31
  32             <tr valign="top">
  33               <td>Authors:</td>
  34
  35               <td>Xiaohua He, Mark D'Ascenzo, Jamie Lyman, and Greg
  36               Martin</td>
  37             </tr>
  38
  39             <tr valign="top">
  40               <td>Date made:</td>
  41
  42               <td>01/99</td>
  43             </tr>
  44
  45             <tr valign="top">
  46               <td>Species:</td>
  47
  48               <td><em>Lycopersicon esculentum</em></td>
  49             </tr>
  50
  51             <tr valign="top">
  52               <td>Accession:</td>
  53
  54               <td>tomato line R11-12 (35S: Pto in Rio Grande x
  55               Money Maker)</td>
  56             </tr>
  57
  58             <tr valign="top">
  59               <td>Resistance:</td>
  60
  61               <td>tomato line is resistant to <em>Pseudomonas
  62               syringae</em> pv. tomato strain T1 that expresses the
  63               avrPto gene</td>
  64             </tr>
  65
  66             <tr valign="top">
  67               <td>Tissue:</td>
  68
  69               <td>leaf</td>
  70             </tr>
  71
  72             <tr valign="top">
  73               <td>Developmental stage:</td>
  74
  75               <td>4 week old plants</td>
  76             </tr>
  77
  78             <tr valign="top">
  79               <td>Vector:</td>
  80
  81               <td>pBluescript SK(+/-)</td>
  82             </tr>
  83
  84             <tr valign="top">
  85               <td>Host:</td>
  86
  87               <td>SOLR</td>
  88             </tr>
  89
  90             <tr valign="top">
  91               <td>Primary pfu:</td>
  92
  93               <td>1.0 x 10<small><sup>6</sup></small></td>
  94             </tr>
  95
  96             <tr valign="top">
  97               <td>Number mass excised:</td>
  98
  99               <td>1.0 x 10<small><sup>7</sup></small></td>
 100             </tr>
 101
 102             <tr valign="top">
 103               <td>Average insert length:</td>
 104
 105               <td>1.0 Kb</td>
 106             </tr>
 107
 108             <tr valign="top">
 109               <td>Cloning sites:</td>
 110
 111               <td>5' EcoRI, 3' XhoI</td>
 112             </tr>
 113
 114             <tr valign="top">
 115               <td>Antibiotic:</td>
 116
 117               <td>ampicillin</td>
 118             </tr>
 119
 120             <tr valign="top">
 121               <td>Primers:</td>
 122
 123               <td>M13F and M13R</td>
 124             </tr>
 125
 126             <tr valign="top">
 127               <td>Comments:</td>
 128
 129               <td>For this library four-week-old plants were
 130               inoculated with the Pst(avrPto) strain and the leaves
 131               were harvested. Two plants were used for each
 132               time-point as follows. High inoculation level:
 133               10<small><sup>8</sup></small> cfumL of Pst(avrPto).
 134               Equal amounts of leaves were harvested at 0, 2, 4, 6,
 135               and 8 hours after inoculation. Low inoculation level:
 136               10<small><sup>5</sup></small> cfumL of Pst(avrPto).
 137               Equal amounts of leaves were harvested at 0, 12, 24,
 138               36, and 48 hours after inoculation. High bacterial
 139               titre gives no HR on R11-12, but results in disease
 140               symptoms beginning at about 48 hours after
 141               inoculation. Low bacterial titre gives no HR on
 142               R11-12, but results in disease symptoms beginning at
 143               about 48 hours after inoculation. Keeping leaves from
 144               each line separate, equal amounts of leaves from each
 145               time-point were pooled and used to extract polyA RNA
 146               using Promega's polyATract mRNA isolation system.
 147               Stratagene's Zap-cDNA synthesis kit was used to
 148               prepare cDNA. The cDNA was cloned into Uni-ZAP XR
 149               vector using the EcoRI site at the 5' end and the
 150               XhoI site at the 3' end. When a sample of each
 151               library was plated on NZY agar plates containing IPTG
 152               and X-gal, the white:blue colony ratio was about
 153               100:1. After mass excision, twenty-five clones from
 154               each library were picked randomly and characterized.
 155               All 50 clones contained inserts with sizes ranging
 156               from 0.5 Kb to more than 3 Kb (avg=1 Kb). The 5' ends
 157               of 14 random clones were sequenced (7 from each
 158               library). The EcoRI site was altered/missing in 11 of
 159               the clones. We expect the R11-12 library will contain
 160               cDNAs corresponding to genes that are induced by the
 161               hypersensitive response and during "field"
 162               resistance. The R11-13 library is expected to contain
 163               cDNAs corresponding to genes that are induced during
 164               the disease susceptibility response.</td>
 165             </tr>
 166           </table>
 167         </td>
 168       </tr>
 169 </table>
 170
 171   </center>
 172 END_HEREDOC
 173 $page->footer();