cgi-bin/content/libraries/callus.pl

   1 use strict;
   2 use CXGN::Page;
   3 my $page=CXGN::Page->new('callus.html','html2pl converter');
   4 $page->header('Callus Library');
   5 print<<END_HEREDOC;
   6
   7   <center>
   8
   9     <table summary="" width="720" cellpadding="0" cellspacing="0"
  10     border="0">
  11       <tr>
  12         <td>
  13           <center>
  14             <h2>Callus Library</h2>
  15           </center>
  16
  17           <table summary="" width="100\%" cellspacing="5">
  18             <tr valign="top">
  19               <td width="20\%">Library:</td>
  20
  21               <td>cLEC</td>
  22             </tr>
  23
  24             <tr>
  25               <td>TIGR ID:</td>
  26
  27               <td>TCA</td>
  28             </tr>
  29
  30             <tr valign="top">
  31               <td>Authors:</td>
  32
  33               <td>Alcala, Vrebalov, White, and Giovannoni</td>
  34             </tr>
  35
  36             <tr valign="top">
  37               <td>Date made:</td>
  38
  39               <td>02/99</td>
  40             </tr>
  41
  42             <tr valign="top">
  43               <td>Species:</td>
  44
  45               <td><em>Lycopersicon esculentum</em></td>
  46             </tr>
  47
  48             <tr valign="top">
  49               <td>Accession:</td>
  50
  51               <td>TA496</td>
  52             </tr>
  53
  54             <tr valign="top">
  55               <td>Tissue:</td>
  56
  57               <td>combined undifferentiated and shooting
  58               callus</td>
  59             </tr>
  60
  61             <tr valign="top">
  62               <td>Developmental stage:</td>
  63
  64               <td>7-10 days post germination</td>
  65             </tr>
  66
  67             <tr valign="top">
  68               <td>Vector:</td>
  69
  70               <td>pBluescript SK(+/-)</td>
  71             </tr>
  72
  73             <tr valign="top">
  74               <td>Host:</td>
  75
  76               <td>SOLR</td>
  77             </tr>
  78
  79             <tr valign="top">
  80               <td>Primary pfu:</td>
  81
  82               <td>1.5 x 10<small><sup>6</sup></small></td>
  83             </tr>
  84
  85             <tr valign="top">
  86               <td>Number mass excised:</td>
  87
  88               <td>5.0 x 10<small><sup>5</sup></small></td>
  89             </tr>
  90
  91             <tr valign="top">
  92               <td>Average insert length:</td>
  93
  94               <td>1.3 Kb</td>
  95             </tr>
  96
  97             <tr valign="top">
  98               <td>Cloning sites:</td>
  99
 100               <td>5' EcoRI, 3' XhoI</td>
 101             </tr>
 102
 103             <tr valign="top">
 104               <td>Antibiotic:</td>
 105
 106               <td>ampicillin</td>
 107             </tr>
 108
 109             <tr valign="top">
 110               <td>Primers:</td>
 111
 112               <td>M13F and M13R</td>
 113             </tr>
 114
 115             <tr valign="top">
 116               <td>Comments:</td>
 117
 118               <td>Cotyledons of seedlings 7-10 days
 119               post-germination were excised, cut at both ends and
 120               placed on MS medium with no selection. Mixed callus
 121               was harvested at 25 and 40 days following placement
 122               on MS medium and included undifferentiated masses,
 123               differentiating shoots, and fully organized shoots in
 124               approximately equal mass.</td>
 125             </tr>
 126           </table>
 127         </td>
 128       </tr>
 129     </table>
 130
 131   </center>
 132 END_HEREDOC
 133 $page->footer();