4 use CXGN
::Page
::FormattingHelpers qw
/info_section_html columnar_table_html info_table_html/;
6 my $page=CXGN
::Page
->new('tomato_project_overview.html','html2pl converter');
7 $page->add_style(text
=> <<EOS);
9 #seqpeople div.org {padding-left: 1em; white-space: nowrap;}
11 $page->header(('International Tomato Sequencing Project Overview') x
2);
12 print info_section_html
( title
=> 'Abstract',
14 <p>The tomato genome is comprised
15 of approximately 950 Mb of DNA - more than 75\% of which
16 is heterochromatin and largely devoid of genes. The
17 majority of genes are found in long contiguous stretches
18 of gene-dense euchromatin located on the distal portions
19 of each chromosome arm. A minimal tiling path of BAC
20 clones will be identified through this approximately 220
21 Mb euchromatin. The starting point for sequencing the
22 genome will be approximately 1500 "seed" BAC clones
23 individually anchored to the tomato high density genetic
24 map based on a single, common L. esculentum x L.
25 pennellii F2 population (referred to as the F2.2000; view
26 <a href="/cview/map.pl?map_id=9">
27 map on SGN</a>). Sequencing will proceed on a BAC-by-BAC
28 basis. Each sequenced anchor BAC will serve as a seed
29 from which to radiate out into the minimum tiling path.
30 Identification of the correct next BACs in the
31 euchromatin minimum tiling path for sequencing will be
32 based on the use of a BAC end sequence database that will
33 be created as part of this project, as well as a
34 fingerprint contig physical map that is currently being
35 constructed. A subset of the sequenced BACs will be
36 localized on pachytene chromosomes via FISH (fluorescence
37 in situ hybridization) to help guide the extension of the
38 tiling path through the euchromatic arms of each
39 chromosome and to determine when the heterochromatin and
40 telomeric regions have been reached on each arm. A
41 bioinformatics portal will be created for this project
42 that will be mirrored at several locations around the
43 world and provide a mechanism by which researchers in
44 different locations can develop and contribute
45 bioinformatics tools and information to the project. A
46 common set of standards for BAC sequencing and finishing,
47 and for gene nomenclature, and structural and functional
48 gene annotation (please refer to the <a href=
49 "/solanaceae-project/index.pl">Solanaceae Project page</a>).
53 print info_section_html
( title
=> 'Objectives',
55 The objectives of the tomato sequencing project are to:
58 <li>produce a contiguous sequence of the gene rich,
59 euchromatic arms of each of the 12 tomato
62 <li>process and annotate this sequence in a manner
63 consistent and compatible with similar data from
64 Arabidopsis, rice and other plant species.</li>
66 <li>create an international bioinformatics portal for
67 comparative Solanaceae genomics which can store,
68 process, and make available to the public the sequence
69 data and derived information from this project and
70 associated genomics activities in other solanaceous
75 print info_section_html
( title
=> 'More Project Documents',
78 <li><a href="/about/tomato_sequencing.pl">International Tomato Genome Sequencing Project Home</a></li>
79 <li><a href="/about/tomato_sequencing_scope.pl">Tomato Sequencing Scope and Completion Criteria</a></li>
80 <li><a href="/solanaceae-project/index.pl">SOL Project</a></li>
84 #given people, agencies, and emails, make html to list them
90 my ($name, $org, $email) = @
$_ == 3 ? @
$_ : ($_->[0],$last_org,$_->[1]);
91 # $org =~ s/([A-Za-z][a-z]*)/ucfirst($1)/eg;
92 $name =~ s/([A-Za-z][a-z]*)/ucfirst($1)/eg;
94 my ($id,$domain) = split /@/,$email;
95 $domain ||= $last_domain;
96 $last_domain = $domain;
97 $email = $id.'@'.$domain;
98 qq|<a
class="person" href
="mailto:$email">$name</a><div class="org">$org</div
>|
102 print info_section_html
103 ( title
=> 'Participants and Funding',
105 .qq|<div align
="right">\n|
106 .info_table_html
( __multicol
=> 2,
107 'Est. Total MBases' => 219, 'Est. Total BACs' => 2276,
118 'Est. Euchromatin Size (Mb)',
122 __align
=> 'ccllccc',
123 __tableattrs
=> 'id="seqpeople" cellspacing="0" cellpadding="0"',
130 ['J. Giovannoni', 'USDA/ARS','jjg33@cornell.edu'],
131 ['B. Roe', 'University of Oklahoma', 'broe@ou.edu' ],
132 ['J. Van Eck','Boyce Thompson Institute','jv27@cornell.edu'],
133 ['L. Mueller','Boyce Thompson Institute','lam87@cornell.edu'],
134 ['S. Stack','Colorado State U.','sstack@lamar.colostate.edu'],
136 'National Science Foundation',
143 people_list
(['D. Choi','KRIBB Seoul','doil@kribb.re.kr'],
144 ['B.D. Kim','Natl. U.','kimbd@snu.ac.kr'],
147 'BioGreen21 Project / RDA<br />Frontier 21 Project / CFCG<br />Ministry of Science and Technology (MOST)',
148 'Feb. 2004, July 2004',
154 people_list
(['C. Li','Chinese Acad. Sci.','lichu@msu.edu'],
155 ['Y. Xue','ybxue@genetics.ac.cn'],
156 ['Z. Cheng','zkcheng'],
157 ['M. Chen','mschen'],
158 ['H. Ling','hqling'],
160 'Chinese Academy of Science<br />Natural Science Foundation',
167 people_list
(['G. Bishop','Imperial College','gdb@aber.ac.uk'],
168 ['G. Seymour','Nottingham University','graham.seymour@hri.ac.uk'],
169 ['G. Bryan', 'SCRI', 'glenn.bryan@scri.ac.uk'],
171 'BBSRC/DEFRA, SEERAD',
178 people_list
(['R.P. Sharma','U. Hyderabad','rpssl@uohd.ernet.in'],
179 ['J. Khurana,','khurana@genomeindia.org'],
180 ['A. Tyagi','akhilesh'],
181 ['N.K. singh','National Research Centre<br />on Plant Biotech., IARI','nksingh@nrcpb.org'],
183 'DBT, Govt. of India',
190 people_list
(['w. stiekema','Centre for<br />Biosystems Genomics','willem.stiekema@cbsg.nl'],
191 ['p. lindhout','Wageningen U.','Pim.Lindhout@wur.nl'],
192 ['t. jesse','KeyGene','Taco.Jesse@keygene.com'],
193 ['R. Klein Lankhorst','Wageningen U.','rene.kleinlankhorst@wur.nl'],
202 people_list
(['m. bouzayen','BMPMF','bouzayen@flora.ensat.fr']),
203 'National Agency for Genome Sequencing',
210 people_list
(['D. Shibata','Kazusa Inst.','shibata@kazusa.or.jp'],
211 ['S. Tabata','tabata'],
220 people_list
(['A. Granell','Inst. de Biologia Molecular<br />y Cellular de Plantas Valencia','agranell@ibmcp.upv.es'],
221 ['M. Botella','U. Malaga','mabotella@uma.es'],
223 'Submitted to Genoma Espana',
234 people_list
(['S. Huang', 'Chinese Academy of Sciences', 'huangsanwen@mac.com']), '', 'Funded',
238 people_list
(['G. Giuliano','ENEA','giovanni.giulianog@enea.it'],
239 ['L. Fruciante','U. Naples','fruscian@unina.it'],
241 'Italian Ministry of Agriculture and Italian Ministry of Research',