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11 Interspecific Reproductive Barriers in Tomato (IRBT)
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17 <img src="/documents/img/secretom/tomato_flower_cropped_120.jpg" />
20 Solanum lycopersicum flower
26 This is an NSF Plant Genome program funded collaborative scientific
27 endeavor to identify and understand the biological processes
28 underlying species isolation within the Tomato family. We are using
29 proteomic tools to study the molecular barriers between tomato
30 species, with particular focus on secreted proteins as these are
31 believed to be critical in establishing such barriers. We have been
32 characterizing the proteomes of pollen, styles and pollinated styles
33 from both Solanum lycopersicum, S. habrochaites and S. pennellii.
38 The Evolutionary Origins of the Plant Cell Wall and Associated Secretome
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45 <img src="/documents/img/secretom/p_margaritaceum_306.png" />
48 Cells of Penium margaritaceum, a member of the Charophycean green
49 alga, following treatment with a microtubule inhibitor. Pectin
50 components of the wall are shown in green. Microscopic imaging and
51 cover design by David Domozych.
57 One of the most important phases of plant evolution occurred
58 approximately 460-480 million years ago when photosynthetic
59 organisms developed the capacity to colonize land. This transition
60 to harsh terrestrial conditions would have required the ability to
61 resist new abiotic stresses, including desiccation and UV radiation,
62 as well as the development of mechanisms to promote progeny
63 dispersal. It is likely that polysaccharide and cuticular cell walls
64 were essential for the transition to a terrestrial environment and
65 yet the evolutionary origins of their biosynthesis are not well
66 understood. In collaboration with Dr. William Willats (University of
67 Copenhagen), Dr. David Domozych (Skidmore College). Dr. Jeffrey
68 Doyle and Dr. Iben Sørensen (Cornell University), we are addressing
69 the hypothesis that the origins of plant cell walls occurred during
70 divergence within the Charophycean green algae (CGA), which
71 represent the closest living relatives to land plants
72 (embryophytes). Characterization of the secretomes of divergent
73 members of the CGA will likely provide insights into the mechanisms
74 that are currently used by embryophytes for primary, secondary and
75 cuticular cell wall formation and restructuring.