3 my $page=CXGN
::Page
->new('germinatingseed.html','html2pl converter');
4 $page->header('Germinating Seed Library');
9 <table summary="" width="720" cellpadding="0" cellspacing="0"
14 <h2>Germinating Seed Library</h2>
17 <table summary="" width="100\%" cellspacing="5">
19 <td width="20\%">Library:</td>
33 <td>Alcala, Vrebalov, White, and Giovannoni</td>
45 <td><em>Lycopersicon esculentum</em></td>
57 <td>whole seedlings</td>
61 <td>Developmental stage:</td>
63 <td>early germinating seedlings/cotyledons</td>
69 <td>pBluescript SK(+/-)</td>
81 <td>3.5 x 10<small><sup>5</sup></small></td>
85 <td>Number mass excised:</td>
87 <td>2.0 10<small><sup>5</sup></small></td>
91 <td>Average insert length:</td>
97 <td>Cloning sites:</td>
99 <td>5' EcoRI, 3' XhoI</td>
111 <td>M13F and M13R</td>
117 <td>Seedlings were collected seven days
118 post-imbibition on water-agar. Mixed-stage, whole,
119 germinating seedlings from seed coat emergence up to
120 two centimeters in length were chosen. Seeds not
121 showing obvious signs of germination were discarded.
122 Tissue consists of germinating seedlings at various
123 stages from 2 days imbibition to emergence
124 (approximately four days) to 1 cm (approximately six
125 days) to 2 cm (approximately 8 days). Tissues were
126 harvested based on stage, not day.</td>