3 my $page=CXGN
::Page
->new('clone_policy.html','html2pl converter');
4 $page->header('Clone Request Policy');
10 <table summary="" width="720" cellpadding="0" cellspacing="0"
14 <h3>Tomato Genome Project - clone distribution policy.</h3>
16 <p>Development of the tomato EST database is
17 currently in progress and continues to be updated on a
18 regular basis as new sequences are added. Our objective
19 is to ultimately make available to the research community
20 a complete set of non-redundant tomato ESTs. The "in
21 progress" collection is continually updated as new EST
22 sequences are added to the database. In some cases new
23 EST sequences provide information that modifies the
24 previous "in-progress" non-redundant EST collection. For
25 example, when clones are found through additional
26 sequencing to be chimeric, or when a new EST sequence
27 "links" two clones previously thought to be unique. In
28 short, the "in-progress" non-redundant set is expected to
29 contain a considerable number of duplications and errors
30 of various types. Once the sequencing phase of this
31 project is completed (fall 2001) a final build will be
32 created and a final non-redundant gene set defined. This
33 collection is currently estimated to range from 25,000 -
34 40,000 cDNA clones which will be condensed, quality
35 confirmed, and re-arrayed into a collection that can be
36 distributed in whole - or can much more efficiently be
37 searched for individual clones than is currently the case
38 with over 25 libraries each containing 4000 - 15,000
41 <p><strong>We realize the need for access by the research
42 community to EST clones prior to completion of this
43 project and thus have instituted a policy whereby up to
44 150 clones per laboratory per year can be requested on a
45 subsidized re-charge basis through the Clemson University
46 Genomics Institute (CUGI) which handles maintenance and
47 distribution of the tomato ESTs and receives funding
48 through this project to offset some of the associated
51 <strong>Cost Basis</strong>
52 <p>CUGI received a total of \$100,000 (apx. \$80K in direct
53 costs) spread over 3 years in support of clone
54 maintenance and distribution. These funds have been used
55 for a) purchase of 3 -80 freezers and service contracts
56 to insure their operation b) support a half-time tech
57 position, c) annual replication of all EST libraries to
58 insure against loss of clone viability, and d) software
59 upgrades used in clone request tracking and eventual
60 public dissemination of all clone requests. It is
61 important to realize that with 22 ordered EST libraries
62 (each containing 4,000 - 15,000 ordered clones) tracking,
63 localizing, and picking each clone represents a
64 significant effort.</p>
66 <strong>Clone Request Charges</strong>
67 <p>Up to 150 clones can be requested per laboratory per year
68 at the subsidized rate of \$5 per clone. An unlimited
69 number of additional clones can be requested by any one
70 laboratory in a 12 month period but at a non-subsidized
71 rate of \$20 per clone. This later price is in line with
72 other clone distribution centers, such as the American
73 Type Culture Collection (ATCC), which recover total costs
74 through re-charge fees. Cost recovery for the entire
75 non-redundant set will be determined when said collection
76 is completed (est. late 2001).</p>
78 <table summary="" border="0">
80 <td>Clones 1 - 150 in any twelve month period:</td>
85 <td>Clones 151 - unlimited in any twelve month
91 <td>Entire 384 well plates of sequential ESTs from a
93 <td>\$10/plate(academic)<br />
94 \$50/plate(industry)</td>
98 <td>Shipping and handling (all clones shipped via
99 over-night courier):</td>
100 <td>apx. \$40/request, depending on specific shipping
105 <p>Clone requests should be directed to <a href=
106 "mailto:atkins2\@genome.clemson.edu">Dr. Michale
107 Atkins</a> at the Clemson University Genomics Institute
110 <strong>Acknowledgements</strong>
111 <p>Publications referring to the use of clones resulting
112 from the NSF Tomato Genome Project should include an
113 acknowledgement similar to the following: "Tomato EST
114 clone(s) XXX were supplied by the NSF Tomato Genome
115 Project (DBI-9872617, S. Tanksley, G. Martin, J.
116 Giovannoni, C. Ronning)".</p>
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