3 my $page=CXGN
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4 $page->header('L. pennellii Introgression lines (ILs)');
9 <table summary="" width="720" cellpadding="0" cellspacing="0"
13 <h3 align="center"><em>L. pennellii</em> Introgression
17 Congenic lines that differ in a single defined chromosome
18 segment are useful for the study of complex phenotypes,
19 as they allow isolation of the effect of a particular
20 quantitative trait locus (QTL) from those of the entire
21 genome. We developed a set of Lycopersicon
22 pennellii-derived introgression lines (ILs) that together
23 cover the entire genome in the background of L.
24 esculentum Var. M82. This resource is very powerful for
25 the study of genes affecting complex phenotypes.</p>
27 <p>The second generation IL population is composed of 76
28 ILs (the 50 original lines and 26 new ILs), each
29 containing a single introgression from L. pennellii (LA
30 716) in the genetic background of the processing tomato
31 variety M82. The IL map was connected to the
32 high-resolution F2 map composed of 1500 markers. This was
33 achieved by probing all of the specific chromosome lines
34 with the RFLP markers from the framework F2 map. A total
35 of 614 markers were probed and the ends of the
36 introgressions were mapped with the resolution of the F2
39 <p>The L. pennellii introgressed segments appear as solid
40 bars in which the boundary edge of each segment is
41 indicated by inclusive (+) and exclusive (-) RFLP
42 markers. All ILs are homozygous for the introgressed
43 segment except for part of IL8-1 (dashed line). Bins are
44 designated by the chromosome number followed by a capital
45 letter and indicate a unique area of IL overlap and
46 singularity; it is important to note that some of the bin
47 designations might change as more probing is done.
48 Molecular and genetic markers are indicated to the right
49 of each chromosome and the genetic distances (in cM)
50 according to Tanksley et al. (1992) are indicated to the
53 <p>Seed of the second generation ILs is presently being
55 "http://tgrc.ucdavis.edu">The C.M. Rick Tomato Genetics Resource
56 Center, University of California Davis</a>
57 and the ILs were assigned accession numbers LA4028 -
61 <li><a href="chr1.pl" target="_blank">Chromosome
64 <li><a href="chr2.pl" target="_blank">Chromosome
67 <li><a href="chr3.pl" target="_blank">Chromosome
70 <li><a href="chr4.pl" target="_blank">Chromosome
73 <li><a href="chr5.pl" target="_blank">Chromosome
76 <li><a href="chr6.pl" target="_blank">Chromosome
79 <li><a href="chr7.pl" target="_blank">Chromosome
82 <li><a href="chr8.pl" target="_blank">Chromosome
85 <li><a href="chr9.pl" target="_blank">Chromosome
88 <li><a href="chr10.pl" target="_blank">Chromosome
91 <li><a href="chr11.pl" target="_blank">Chromosome
94 <li><a href="chr12.pl" target="_blank">Chromosome
98 <p><u>IL References</u></p>
100 <p>Eshed Y, M Abu-Abied, Y Saranga, D Zamir (1992)
101 Lycopersicon esculentum lines containing small
102 overlapping introgressions from L. pennellii. Theor Appl
103 Genet 83:1027-1034</p>
105 <p>Eshed Y and D. Zamir (1994) Introgressions from
106 Lycopersicon pennellii can improve the soluble-solids
107 yield of tomato hybrids. Theor Appl Genet 88:891-897.</p>
109 <p>Eshed Y and D. Zamir (1994) A genomic library of
110 Lycopersicon pennellii in L. esculentum: A tool for fine
111 mapping of genes. Euphytica 79:175-179.</p>
113 <p>Eshed Y and D Zamir (1995) An introgression line
114 population of Lycopersicon pennellii in the cultivated
115 tomato enables the identification and fine mapping of
116 yield associated QTL. Genetics 141:1147-1162.</p>
118 <p>Eshed Y and D Zamir (1996) Less than additive
119 epistatic interactions of QTL in tomato. Genetics
122 <p>Eshed Y, G Gera and D Zamir (1996) A genome-wide
123 search for wild-species alleles that increase
124 horticultural yield of processing tomatoes Theor Appl
125 Genet 93: 877-886.</p>
127 <p>Zamir D and Y Eshed (1998) Tomato genetics and
128 breeding using nearly isogenic introgression lines
129 derived from wild species. in: Molecular Dissection of
130 Complex Traits. ed. AH Paterson. CRC Press Inc. Fl.
133 <p>Qilin P, Yong-Sheng L, Budai-Hadrian O, Sela M,
134 Carmel-Goren L, Zamir D and R Fluhr (2000) Comparative
135 genetics of NBS-LRR resistance gene homologues in the
136 genomes of two dicotyledons: tomato and Arabidopsis.
137 Genetics 155: 309-322.</p>
139 <p>Fridman E, Pleban T and D Zamir (2000) A recombination
140 hotspot delimits a wild species QTL for tomato sugar
141 content to 484-bp within an invertase gene. Proc Natl
142 Acad Sci USA 97: 4718-4723.</p>